Summary of project PR002436
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002436. The data can be accessed directly via it's Project DOI: 10.21228/M8RN89 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR002436 |
| Project DOI: | doi: 10.21228/M8RN89 |
| Project Title: | Respiration defects limit serine synthesis required for lung cancer growth and survival |
| Project Type: | Pool Size Metabolomic in vivo |
| Project Summary: | Mitochondrial function supports energy and anabolic metabolism. Pathogenic mitochondrial DNA (mtDNA) mutations impair these processes, causing mitochondrial diseases. Their role in human cancers is less clear; while some cancers harbor high mtDNA mutation burden, others do not. Here we show that a proofreading mutant of DNA polymerase gamma (PolGD256A) increases the mtDNA mutation burden in non-small-cell lung cancer (NSCLC). This mutation promotes the accumulation of defective mitochondria, reduces tumor cell proliferation and viability, and improves cancer survival. In NSCLC, pathogenic mtDNA mutations enhance glycolysis and create a glucose dependency to support mitochondrial energy, but at the expense of a lower NAD⁺/NADH ratio that hinders de novo serine synthesis. Thus, mitochondrial function in NSCLC is essential for maintaining adequate serine synthesis, which in turn supports the anabolic metabolism and redox homeostasis required for tumor growth, explaining why these cancers preserve functional mtDNA. |
| Institute: | Rutgers University |
| Department: | Rutgers Cancer Institute |
| Laboratory: | Eileen White |
| Last Name: | Cararo Lopes |
| First Name: | Eduardo |
| Address: | 195 Little Albany Street |
| Email: | edu.llopes@gmail.com |
| Phone: | 732-235-5795 |
| Funding Source: | NIH |
| Publications: | Respiration defects limit serine synthesis required for lung cancer growth and survival |
Summary of all studies in project PR002436
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST003883 | Respiration defects limit serine synthesis required for lung cancer growth and survival - Effect of Polg mutation in NSCLC Tissues | Mus musculus | Rutgers University | MS | 2025-05-06 | 1 | 219 | Uploaded data (12.3G)* |
| ST003884 | Respiration defects limit serine synthesis required for lung cancer growth and survival - Effect of Polg mutation in NSCLC Em tumor derived cell lines (TDCLs) | Mus musculus | Rutgers Cancer Institute | MS | 2025-05-06 | 1 | 108 | Uploaded data (6.6G)* |
| ST003885 | Respiration defects limit serine synthesis required for lung cancer growth and survival - Effect of Polg mutation in NSCLC tumor derived lines (TDCLs) | Mus musculus | Rutgers University | MS | 2025-05-06 | 1 | 34 | Uploaded data (3G)* |
| ST003886 | Respiration defects limit serine synthesis required for lung cancer growth and survival - Effect of Polg mutation in NSCLC TDCLs | Mus musculus | Rutgers Cancer Institute | MS | 2025-05-06 | 1 | 48 | Uploaded data (2.7G)* |
| ST003887 | Respiration defects limit serine synthesis required for lung cancer growth and survival - Effect of Polg mutation in NSCLC conditioned medium | Mus musculus | Rutgers Cancer Institute | MS | 2025-05-06 | 1 | 148 | Uploaded data (7.8G)* |
| ST003888 | Respiration defects limit serine synthesis required for lung cancer growth and survival - Effect of Polg mutation in NSCLC Tissues_Muscle | Mus musculus | Rutgers University | MS | 2025-05-06 | 1 | 51 | Uploaded data (3G)* |
| ST003889 | Respiration defects limit serine synthesis required for lung cancer growth and survival - Effect of Polg mutation in NSCLC Tissues_Lung | Mus musculus | Rutgers University | MS | 2025-05-06 | 1 | 49 | Uploaded data (3.2G)* |
| ST003890 | Respiration defects limit serine synthesis required for lung cancer growth and survival - Effect of Polg mutation in NSCLC Tissues_Liver | Mus musculus | Rutgers University | MS | 2025-05-06 | 1 | 51 | Uploaded data (3.4G)* |
| ST003891 | Respiration defects limit serine synthesis required for lung cancer growth and survival - Effect of Polg mutation in NSCLC Tissues_Plasma | Mus musculus | Rutgers University | MS | 2025-05-06 | 1 | 51 | Uploaded data (3.4G)* |
| ST003893 | Respiration defects limit serine synthesis required for lung cancer growth and survival - NSCLC rewires glycolytic metabolism to synthesize more serine from glucose (Tumors) | Mus musculus | Rutgers University | MS | 2025-05-06 | 1 | 47 | Uploaded data (2.9G)* |
