Summary of project PR002443
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002443. The data can be accessed directly via it's Project DOI: 10.21228/M8VG1V This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR002443 |
| Project DOI: | doi: 10.21228/M8VG1V |
| Project Title: | Hypertonic stress promotes intracellular lipid accumulation via glutamine anaplerosis |
| Project Type: | Untargeted lipidomics |
| Project Summary: | In this work, we describe that acute hypertonic stress triggers the upregulation of metabolic pathways that promote glutamine-dependent lipid accumulation associated with decreased oxygen consumption thus increasing potential water storage. Motive: We sought to understand the lipidomic profile of cells exposed to hypertonic stress, as hypertonic stress is known to regulate lipid metabolism. Methods: We treated mouse kidney inner medullary collecting duct cells with 100 mmol NaCl for 12 hours and then collected cell lysates for evaluation of the lipidomic profile via LC-MS/MS Samples: Control (n=4) – no exposure to 100 mmol NaCl – treated with DMEM low glucose no FBS or antibiotic/antimycotic Salt (n=5) – Exposed to 100 mmol NaCl – treated with DMEM low glucose with no FBS or antibiotic/antimycotic. Results: We found that long chain triglycerides and cardiolipins are increased after exposure to NaCl. |
| Institute: | Vanderbilt University |
| Department: | Chemistry |
| Laboratory: | Center for Innovative Technology |
| Last Name: | Leaptrot |
| First Name: | Katrina |
| Address: | 7300 Stevenson Center Lane |
| Email: | katrina.l.leaptrot@vanderbilt.edu |
| Phone: | 6158228422 |
Summary of all studies in project PR002443
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST003903 | Hypertonic stress promotes intracellular lipid accumulation via glutamine anaplerosis | Mus musculus | Vanderbilt University | MS* | 2025-10-30 | 1 | 18 | Uploaded data (24.4G)* |
