Summary of project PR002449
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002449. The data can be accessed directly via it's Project DOI: 10.21228/M82Z52 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR002449 |
| Project DOI: | doi: 10.21228/M82Z52 |
| Project Title: | Metabolomic profiling of glycogenolysis-derived G1P promotes pentose phosphate pathway in CD8+ memory T cell |
| Project Summary: | Glucose-6-phosphate (G6P) is a key metabolic molecule that regulates reactive oxygen species (ROS) homeostasis by initiating the pentose phosphate pathway (PPP) to generate NADPH that converts H2O2 to water by providing hydrogen. While both glucose phosphorylation and glycogenolysis result in G6P production, here we show that G6P derived from glycogenolysis, rather than glucose phosphorylation, flows to PPP for ROS clearance in CD8+ memory T (Tm) cells and inflammatory macrophages. Mechanistically, glycogenolysis-produced G1P allosterically induces G6P dehydrogenase (G6PD) binding to glycogen, which together undergo liquid-liquid phase separation (LLPS) and recruit PPP enzymes, resulting in a compartmentalized reaction cascade. Based on mechanistic elucidation, we demonstrated that G1P can act as an antitumor immunotherapeutic agent by modulating memory fitness and maintenance of tumor-reactive CD8+ T cells in mice. These findings revealed an unusual function of glycogen metabolism, which is of paramount importance in the regulation of PPP and redox homeostasis in cells. |
| Institute: | Peking Union Medical College |
| Last Name: | Zhang |
| First Name: | Chaoying |
| Address: | No. 5, Dongdan santiao, Dongcheng District, Beijing, Beijing, Beijing, 100005, China |
| Email: | zhouyaboqq@gmail.com |
| Phone: | 860169156464 |
Summary of all studies in project PR002449
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST003914 | G1P promotes pentose phosphate pathway in CD8+ memory T cells via glycogen-G6PD phase separation and compartmentalization | Mus musculus | Peking Union Medical College | MS | 2025-05-26 | 1 | 182 | Uploaded data (21.7G)* |
