Summary of project PR002462
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002462. The data can be accessed directly via it's Project DOI: 10.21228/M8D83H This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR002462 |
| Project DOI: | doi: 10.21228/M8D83H |
| Project Title: | Acylated putrescine therapeutic discovery for Inflammatory Bowel Diseases |
| Project Summary: | Gut microbial metabolites influence immune responses and play a key role in regulating host metabolism. Inflammatory bowel diseases (IBD) patients have altered gut microbial metabolic signatures driven by shifts in the microbiome. However, the microbial metabolites driving inflammation and remission and their immunomodulatory roles remain largely uncharacterized. We prioritized metabolic features from untargeted IBD fecal metabolomics and selected candidate features based on their microbial association for structural elucidation. We uncovered four acylated putrescines that are IBD-associated and microbially-dependent and investigated their bioactivity. One acylated putrescine, N-oleoylputrescine (NOP), has anti-inflammatory activity and suppressed type 1 immune responses. NOP ameliorated inflammation in several colitis models, modulating myeloid and lymphocyte cell populations and dampening proinflammatory responses, relevant for resolution of inflammation and maintenance of remission in IBD. We present an approach to identify clinically-relevant microbial metabolites from untargeted metabolomics useful for the discovery of bioactive molecules in IBD and other diseases involving host-microbial interactions. |
| Institute: | Broad Institute of MIT and Harvard |
| Last Name: | Avila-Pacheco |
| First Name: | Julian |
| Address: | 415 Main Street, Cambridge, MA 02142 USA |
| Email: | jravilap@broadinstitute.org |
| Phone: | +1 (617) 714-1729 |
| Contributors: | Sena Bae, Julian Avila-Pacheco, Slater L. Clay, Sunghee Bang, Monia Michaud, Diogo Fonseca-Pereira, Eunyoung Chun, Y. Grace Cao, Yancong Zhang, Amrisha Bhosle, Rosa M. Perez, Gleb Pishchany, Hera Vlamakis, Natalia Andreeva, Geniver El Tekle, Xochitl C. Morgan, Jonathan N. Glickman, Ramnik J. Xavier, Daniel B. Graham, Clary B. Clish, Jon Clardy, Eric A. Franzosa, Curtis Huttenhower, and Wendy S. Garrett |
Summary of all studies in project PR002462
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST003930 | Acylated putrescine therapeutic discovery for Inflammatory Bowel Diseases: HILIC-pos profiling of mouse fecal samples | Mus musculus | Broad Institute of MIT and Harvard | MS* | 2025-06-17 | 1 | 28 | Uploaded data (5.9G)* |
| ST003931 | Acylated putrescine therapeutic discovery for Inflammatory Bowel Diseases: HILIC-neg, C8-pos and C18-neg profiling of mouse fecal samples | Mus musculus | Broad Institute of MIT and Harvard | MS* | 2025-10-05 | 1 | 21 | Uploaded data (10.6G)* |
