Summary of project PR002489
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002489. The data can be accessed directly via it's Project DOI: 10.21228/M8X267 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR002489 |
| Project DOI: | doi: 10.21228/M8X267 |
| Project Title: | Trehalose catalytic shift inherently enhances phenotypic heterogeneity and multidrug resistance in Mycobacterium tuberculosis |
| Project Summary: | Drug-resistance (DR) in bacteria often develops through the repetitive formation of drug-tolerant persister cells, which survive antibiotics without genetic changes. It is unclear whether Mycobacterium tuberculosis (Mtb), the bacterium that causes tuberculosis (TB), undergoes a similar transitioning process. Recent studies highlight changes in trehalose metabolism as crucial for Mtb persister formation and drug resistance. In this study, we observe that mutants lacking trehalose catalytic shift activity exhibited fewer DR mutants due to decreased persisters. This shift enhances Mtb survival during antibiotic treatment by increasing metabolic heterogeneity and drug tolerance, facilitating drug-resistance. Rifampicin (RIF)-resistant bacilli display cross-resistance to other antibiotics linked to higher trehalose catalytic shift, explaining how multidrug resistance (MDR) can follow RIF-resistance. In particular, the HN878 W-Beijing strain exhibits higher trehalose catalytic shift, increasing MDR risk. Both genetic and pharmacological inactivation of this shift reduces persister formation and MDR development, suggesting trehalose catalytic shift as a potential therapeutic target to combat TB resistance. |
| Institute: | University of Southern California |
| Last Name: | Eoh |
| First Name: | Hyungjin |
| Address: | 1501 San Pablo Street, Los Angeles, CA, 90033, USA |
| Email: | heoh@usc.edu |
| Phone: | 323-442-6048 |
Summary of all studies in project PR002489
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST003973 | Trehalose catalytic shift inherently enhances phenotypic heterogeneity and multidrug resistance in Mycobacterium tuberculosis | Mycobacterium tuberculosis | University of Southern California | MS | 2025-07-03 | 1 | 900 | Uploaded data (8.9G)* |
