Summary of project PR002507
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002507. The data can be accessed directly via it's Project DOI: 10.21228/M8KN8R This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR002507 |
| Project DOI: | doi: 10.21228/M8KN8R |
| Project Title: | A deep, quantitative lipid atlas of extracellular vesicles across multiple cell lines |
| Project Summary: | Extracellular vesicles (EVs) are defined as subcellular particles surrounded by a lipid bilayer membrane and incorporating various additional biomolecules derived from their donor cell, such as proteins and nucleic acid species. In many disease contexts circulating EVs have received increasing scientific attention due to their potential diagnostic and prognostic value. Additionally, EVs have been ascribed a range of biological functions, ranging from cellular waste disposal to sophisticated, intercellular communication. Consequently, EVs involved in pathological processes may represent therapeutic targets, whereas EV-based therapeutics are being developed for targeted delivery of molecular cargoes in vivo. Detailed knowledge of the molecular make-up of natural EVs derived from diverse tissue and cellular origins is crucial to identify biomarkers, dissect EV functions, and optimize EV engineering for therapeutic purposes. Although the lipid composition of biological membranes has a significant impact on their biophysical and -chemical properties and may affect signaling and interactions at the molecular and cellular level, relatively little is known about the lipid composition of EV membranes. Here, we applied high resolution mass spectrometry to deeply and quantitatively characterize the lipidome of EVs isolated from a panel of malignant and non-malignant cell lines, providing a comprehensive data resource for biomarker research and EV engineering efforts. Furthermore, subset comparisons indicate striking differences between lipid profiles of EVs isolated from cells of different tissue origin, suggesting distinct membrane characteristics that could affect EV biodistribution and function in vivo. |
| Institute: | European Molecular Biology Laboratory |
| Department: | EMBL Heidelberg |
| Last Name: | Drotleff |
| First Name: | Bernhard |
| Address: | Meyerhofstr. 1, Heidelberg, BW, 69117, Germany |
| Email: | bernhard.drotleff@embl.de |
| Phone: | none |
| Publications: | in preparation |
| Contributors: | Alperen Acari, Pragati Lodha, Selin Özhan, Shruthi Hemanna, Jochen Rieck, Bernhard Drotleff, Lothar C. Dieterich |
Summary of all studies in project PR002507
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST004002 | A deep, quantitative lipid atlas of extracellular vesicles across multiple cell lines | Mus musculus | European Molecular Biology Laboratory | MS | 2025-07-21 | 1 | 50 | Uploaded data (14.6G)* |
