Summary of project PR002513
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002513. The data can be accessed directly via it's Project DOI: 10.21228/M8T536 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR002513 |
| Project DOI: | doi: 10.21228/M8T536 |
| Project Title: | SLC45A4 encodes a peroxisomal putrescine transporter that promotes GABA de novo synthesis |
| Project Type: | Cellular polar metabolomics |
| Project Summary: | Solute carriers (SLC) are membrane proteins that facilitate the transportation of ions and metabolites across either the plasma membrane or the membrane of intracellular organelles. With more than 450 human genes annotated as SLCs, many of them are still orphan transporters without known biochemical functions. We developed a metabolomic-transcriptomic association analysis, and we found that the expression of SLC45A4 has a strong positive correlation with the cellular level of γ-aminobutyric acid (GABA). Using mass spectrometry and the stable isotope tracing approach, we demonstrated that SLC45A4 promotes GABA de novo synthesis through the Arginine/Ornithine/Putrescine (AOP) pathway. SLC45A4 functions as a putrescine transporter localized to the peroxisome membrane to facilitate GABA production. Taken together, our results revealed a new biochemical mechanism where SLC45A4 controls GABA production. |
| Institute: | Rutgers University |
| Department: | Department of Medicine |
| Last Name: | Su |
| First Name: | Xiaoyang |
| Address: | 7118 Clinical Academic Building |
| Email: | xs137@rwjms.rutgers.edu |
| Phone: | 7322355447 |
| Funding Source: | R01 GM149664 |
Summary of all studies in project PR002513
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST004015 | SLC45A4 encodes a peroxisomal putrescine transporter that promotes GABA de novo synthesis | Homo sapiens | Rutgers University | MS* | 2025-07-07 | 1 | 12 | Uploaded data (1.1G)* |
| ST004092 | SLC45A4 Knockout cells have lowered GABA production | Homo sapiens | Rutgers University | MS | 2025-08-06 | 1 | 23 | Uploaded data (1.9G)* |
| ST004094 | ODC1 overexpression enhances GABA production but does not fully rescue the effect of SLC45A4 KO. | Homo sapiens | Rutgers University | MS | 2025-08-06 | 1 | 36 | Uploaded data (3.2G)* |
| ST004097 | ODC1 overexpression increased the extracellular putrescine concentration in both WT and SLC45A4 KO cells | Homo sapiens | Rutgers University | MS | 2025-08-07 | 1 | 24 | Uploaded data (1.9G)* |
