Summary of project PR002592
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002592. The data can be accessed directly via it's Project DOI: 10.21228/M8M54Q This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR002592 |
| Project DOI: | doi: 10.21228/M8M54Q |
| Project Title: | Dietary cysteine enhances intestinal stemness via CD8+ T cell-derived IL22 |
| Project Summary: | A fundamental question in physiology is understanding how tissues adapt and alter their cellular composition in response to dietary cues. The mammalian small intestine is maintained by rapidly renewing Lgr5+ intestinal stem cells (ISCs) that respond to macronutrient changes such as fasting regimens and obesogenic diets, yet how specific amino acids control ISC function during homeostasis and injury remains unclear. Here we demonstrate that dietary cysteine, a semi-essential amino acid, enhances ISC-mediated intestinal regeneration following injury. To explore the metabolic pathways influenced by cysteine in the small intestine and colon, we performed metabolomics analysis using high-pressure liquid chromatography-mass spectrometry (HPLC-MS). Cysteine contributes to Coenzyme A (CoA) biosynthesis in intestinal epithelial cells, which promotes expansion of intraepithelial CD8αβ+ T cells and their production of interleukin-22 (IL-22). This enhanced IL-22 signaling directly augments ISCs reparative capacity after injury. |
| Institute: | Koch Institute For Integrative Cancer Research at MIT |
| Last Name: | CHI |
| First Name: | FANGTAO |
| Address: | 500 Main St building 76, Cambridge, MA 02139, USA. |
| Email: | ftchizju@gmail.com |
| Phone: | (617) 324-2577 |
Summary of all studies in project PR002592
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST004124 | Dietary cysteine enhances intestinal stemness via CD8+ T cell-derived IL22 | Mus musculus | Massachusetts Institute of Technology | MS | 2025-09-11 | 1 | 96 | Uploaded data (1.9G)* |
