Summary of project PR002613
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002613. The data can be accessed directly via it's Project DOI: 10.21228/M8WK0T This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR002613 |
| Project DOI: | doi: 10.21228/M8WK0T |
| Project Title: | Development of plant extracts as substrates for untargeted transporter substrate identification in Xenopus oocytes |
| Project Type: | Research article |
| Project Summary: | Amongst the thousands of transport proteins constituting approximately 10% of coding sequences in a genome, only few have an assigned function. Heterologous expression of transporters in combination with the use of plant extracts as complex mixtures of substrates can be a powerful tool for untargeted identification of plant transporter functionality. In this study, we developed and evaluated four extraction protocols to generate Arabidopsis thaliana seedling extracts for use as substrate mixtures in high-throughput screening of transporters expressed in Xenopus laevis oocytes, a well-established system for transporter studies. Our study highlights the importance of developing metabolically diverse yet low-toxicity plant extracts as a critical step toward advancing plant transporter substrate screening. The optimized extraction protocol in combination with X. laevis oocyte assays provide a robust platform for the functional characterization of plant transporters, paving the way for deeper insights into plant physiology and metabolism. |
| Institute: | University of Copenhagen |
| Department: | Plant Molecular Biology |
| Last Name: | Theodorou |
| First Name: | Christos |
| Address: | Thorvaldsensvej 40, Copenhagen, Frederiksberg C, 1871, Denmark |
| Email: | ctheodot@plen.ku.dk |
| Phone: | 52630800 |
| Funding Source: | The present work was supported by The Villum Foundation (#37798) grant awarded to Villum Investigator Professor Barbara Ann Halkier. |
Summary of all studies in project PR002613
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST004151 | Development of plant extracts as substrates for untargeted transporter substrate identification in Xenopus oocytes | Arabidopsis thaliana | University of Copenhagen | MS* | 2025-09-02 | 1 | 167 | Uploaded data (35.4G)* |
