Summary of project PR002624
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002624. The data can be accessed directly via it's Project DOI: 10.21228/M8G84H This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR002624 |
| Project DOI: | doi: 10.21228/M8G84H |
| Project Title: | Equilibrative Nucleoside Transporter 2 Modulates Inosine Catabolism to Influence Astrocyte Metabolism and Reactivity |
| Project Summary: | Astrocytes are central regulators of brain function and exhibit diverse reactive states in response to injury and inflammation. Although the mechanisms underlying these states remain debated, cellular energy metabolism is thought to play a crucial role. This project aimed to define the role of equilibrative nucleoside transporter 2 (Ent2) in regulating astrocytic responses to inflammatory stimulation, with a particular focus on its function in inosine metabolism and energy balance. Using mouse primary astrocytes exposed to cytokine-induced activation, together with targeted metabolomic profiling and pharmacological manipulation, we examined how Ent2 activity influences purine metabolism and reactivity. Our findings show that inosine is an essential energy substrate during inflammation and that Ent2 is required to regulate inosine efflux and maintain ATP production. Loss of Ent2, or pharmacological inhibition of inosine catabolism, disrupted energy homeostasis, impaired mitochondrial function, and enhanced astrocytic reactivity. These results establish an Ent2-inosine-PNP axis as a critical pathway linking purine metabolism to astrocytic reactivity. |
| Institute: | Institute of Biomedical Sciences, Academia Sinica |
| Last Name: | Chern |
| First Name: | Yijuang |
| Address: | 128 Section 2, Academia Road, Nankang, Taipei, 115201, Taiwan |
| Email: | bmychern@ibms.sinica.edu.tw |
| Phone: | +886-2-2652-3913 |
Summary of all studies in project PR002624
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST004164 | Equilibrative Nucleoside Transporter 2 Modulates Inosine Catabolism to Influence Astrocyte Metabolism and Reactivity | Mus musculus | Institute of Biomedical Sciences, Academia Sinica | MS | 2025-09-30 | 1 | 80 | Uploaded data (212M)* |
