Summary of project PR002670
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002670. The data can be accessed directly via it's Project DOI: 10.21228/M8HZ7V This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR002670 |
| Project DOI: | doi: 10.21228/M8HZ7V |
| Project Title: | Constitutive metabolite profiling of European and Asian Fraxinus with varying susceptibility to ash dieback |
| Project Summary: | Hymenoscyphus fraxineus is an invasive pathogen native to East Asia, responsible for the widespread mortality of European ash (Fraxinus excelsior) throughout Europe. Asian ash species, which co-evolved with H. fraxineus, are considered more tolerant than European ash. However, within European ash populations, a small proportion of genotypes show low susceptibility to the pathogen. This study sought to characterize the underlying defence mechanisms to H. fraxineus by performing untargeted constitutive metabolomics profiling of phloem and leaf tissue of susceptible and tolerant European ash and three Asian ash species. Here we report 57 and 36 compounds associated with lower or higher disease susceptibility, from phloem and leaf tissue, respectively. Flavonoids and coumarins were the main classes of detected compounds. In particular, quercitrin and fraxetin exhibited greater variation among the groups. In phloem tissue, quercitrin and fraxetin were more abundant in tolerant than in susceptible European ash and, lowest in Asian ash species. In leaves, however, quercitrin was highest in Asian ash, followed by tolerant and then susceptible European ash. Other flavonoids, coumarins, and iridoid glycosides also showed variation among groups, with stronger differences in phloem than in leaves. Overall, this study advances our understanding of metabolite composition in Fraxinus species with different co-evolutionary histories and susceptibility to H. fraxineus and demonstrates the potential of untargeted metabolomics for investigating defence-related mechanisms in plant-pathogen interactions. |
| Institute: | University of Copenhagen |
| Last Name: | Crocoll |
| First Name: | Christoph |
| Address: | Thorvaldsensvej 40, Frederiksberg, Hovedstaden, 1871, Denmark |
| Email: | chcr@plen.ku.dk |
| Phone: | +45 35 33 33 69 |
| Funding Source: | The study was financially supported by Sveaskog within the framework of the project aimed at ash and elm preservation, Sveriges lantbruksuniversitet Skogsskadecentrum, Kungl. Skogs och Lantbruksakademien (KSLA) grant number CF2022-0009, Partnerskap Alnarp, Stiftelsen för Strategisk Forskning, Stiftelsen fonden för skogsvetenskaplig forskning, Tranemåla stiftelsen, and Extensus stiftelsen. |
| Publications: | Constitutive metabolite profiling of European and Asian Fraxinus with varying susceptibility to ash dieback |
| Contributors: | Tolio, Beatrice; Sherwood, Patrick; Marčiulynienė, Diana; Crocoll, Christoph; Cleary, Michelle; Liziniewicz, Mateusz |
Summary of all studies in project PR002670
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST004232 | Constitutive metabolite profiling of European and Asian Fraxinus with varying susceptibility to ash dieback | Fraxinus excelsior, Fraxinus chinensis, Fraxinus mandshurica, Fraxinus platypoda | Swedish University of Agricultural Sciences | MS* | 2025-10-06 | 1 | 127 | Uploaded data (71.3G)* |
