Summary of project PR002717
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002717. The data can be accessed directly via it's Project DOI: 10.21228/M8FV8K This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR002717 |
| Project DOI: | doi: 10.21228/M8FV8K |
| Project Title: | Interactions between phloem microbiota and metabolomes in three North American ash species (Fraxinus spp.) susceptible to Emerald Ash Borer (Agrilus planipennis) |
| Project Type: | Widely Targeted Metabolomics for Plants |
| Project Summary: | This study aimed to profile microbial communities associated with ash phloem and Emerald Ash Borer (EAB; Agrilus planipennis) larval guts and their relationship to ash phloem metabolites in three native susceptible North American ash species: Fraxinus pennsylvanica (green ash), F. nigra (black ash) and F. americana (white ash) using metabarcoding and widely targeted metabolomics to establish the first global metabolomic profile of phloem in these ash species. We examined interspecies differences in microbiota and metabolite profiles and interactions of ash phloem microbiota and metabolites in relation to EAB infestation. Samples of uninfested phloem, with no visible EAB infestation, but located adjacent to an EAB gallery (PhloemA – Pa) and infested phloem from an EAB gallery (Gallery – G), each represented by two pooled phloem punch samples per tree, were collected from four trees of each ash species showing high EAB infestation signs to track the ‘within-tree’ metabolite variation in response to EAB infestation. We found that microbiota and metabolites in green ash showed a distinct response to EAB infestation compared to the other ash species. We also identified specific metabolites interacting with microbial communities in ash phloem and/or the EAB larval gut. Green ash also displayed a distinct global metabolite profile from the other two species and had the highest number of differentially regulated metabolites. However, green and white ash shared a strong upregulation of terpenoid compounds, several of which were among compounds significantly associated with microbial communities in green ash phloem or the EAB larval gut. |
| Institute: | Cornell University |
| Department: | College of Agricultural and Life Sciences |
| Laboratory: | Bushley Lab |
| Last Name: | Bushley |
| First Name: | Kathryn |
| Address: | 538 Tower Road, Ithaca, NY, 14853 |
| Email: | keb45@cornell.edu |
| Phone: | (607) 255-1276 |
| Funding Source: | NSF |
| Publications: | Environmental Microbiome (in review) |
| Contributors: | Judith Mogouong, Claire Yager, Kathryn Bushley |
Summary of all studies in project PR002717
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST004301 | Metabolomic profiling of three native North American ash trees (Fraxinus spp.) and their relationship to the Emerald ash borer (Agrilus planipennis) infestation | Fraxinus pennsylvanica, Fraxinus nigra, Fraxinus americana | Cornell University | MS | 2025-10-24 | 1 | 24 | Uploaded data (35.1M)* |
