Summary of project PR002721
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002721. The data can be accessed directly via it's Project DOI: 10.21228/M8XV8X This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR002721 |
| Project DOI: | doi: 10.21228/M8XV8X |
| Project Title: | Lactate mitochondrial oxidation drives stemness potential in metastatic breast cancer |
| Project Summary: | Metastatic cancer cells, originating from cancer stem cells with metastatic capacity, utilize nutrient flexibility to navigate the challenges of the metastatic cascade. However, the nutrient required to maintain the stemness potentials of metastatic cancer cells remains unclear. Here, we reveal that metastatic breast cancer cells sustain stemness and initiate metastasis upon detachment by taking up and oxidizing lactate. In detached metastasizing breast cancer cells, lactate is incorporated into the tricarboxylic acid cycle, boosting oxidative phosphorylation, and promoting the stemness potentials via α-KG-DNMT3B-mediated SOX2 hypomethylation. Moreover, lactate is taken up and oxidized in mitochondria by the CD147/MCT1/LDHB complex, which correlates with stemness potentials and tumor metastasis in patients with breast cancer. An intracellularly expressed single-chain variable fragment targeting mitochondrial CD147 (mito-CD147 scFv) effectively disrupts the mitochondrial CD147/MCT1/LDHB complex, inhibits lactate-induced stemness potential, depletes circulating breast cancer cells, and reduces metastatic burden, suggesting promising clinical applications in reducing lactate-fueled metastasis. |
| Institute: | Fourth Military Medical University |
| Last Name: | Zhang |
| First Name: | Jia-Jia |
| Address: | No. 169 Changle West Road, Xincheng District, Xi'an City, Shaanxi Province |
| Email: | 1240568116@qq.com |
| Phone: | +86 156 1927 6681 |
Summary of all studies in project PR002721
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST004305 | Lactate mitochondrial oxidation drives stemness potential in metastatic breast cancer | Homo sapiens | Fourth Military Medical University | MS | 2025-11-07 | 1 | 12 | Uploaded data (513.4M)* |
