Summary of project PR002722
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002722. The data can be accessed directly via it's Project DOI: 10.21228/M8T54K This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR002722 |
| Project DOI: | doi: 10.21228/M8T54K |
| Project Title: | Comparative Metabolomic Profiling of Bone Marrow-Derived Macrophages from Wild-Type and MG53-KO Mice |
| Project Summary: | Tumor-associated macrophages (TAMs) are pivotal players in the tumor microenvironment. Our preliminary research has identified the expression of MG53 (Mitsugumin 53) in TAMs. Through a series of in vitro and in vivo experiments, we have initially demonstrated that the genetic ablation of MG53 drives macrophages toward a pro-tumoric M2-like phenotypic polarization. The primary objective of this project was to investigate the underlying metabolic reprogramming associated with MG53 deficiency in macrophages. We hypothesized that the MG53-mediated M2 polarization would be underpinned by a distinct metabolic profile. This study aimed to comprehensively characterize the metabolomic landscape of bone marrow-derived macrophages (BMDMs) from MG53-knockout (KO) mice compared to their wild-type (WT) littermates to validate this hypothesis at the metabolic level. Our metabolomic analysis successfully revealed a definitive M2-like metabolic signature in MG53-KO BMDMs. This provides crucial functional validation of our prior findings and establishes a direct link between MG53 and metabolic reprogramming in macrophage polarization. These results position MG53 as a novel and critical regulator at the nexus of immunometabolism, suggesting its potential as a therapeutic target for modulating macrophage function in cancer. |
| Institute: | Zhejiang University |
| Last Name: | Pei |
| First Name: | Yumeng |
| Address: | Wu Tong Road Number 366, HangZhou, Zhejiang, China, 310027 |
| Email: | 0623B01@zju.edu.cn |
| Phone: | (0571)87236114 |
Summary of all studies in project PR002722
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST004306 | Comparative Metabolomic Profiling of Bone Marrow-Derived Macrophages from Wild-Type and MG53-KO Mice | Mus musculus | Zhejiang University | MS* | 2025-10-28 | 1 | 8 | Uploaded data (9.1G)* |
