Summary of project PR002768
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002768. The data can be accessed directly via it's Project DOI: 10.21228/M8VR9N This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR002768 |
| Project DOI: | doi: 10.21228/M8VR9N |
| Project Title: | Medium chain triglycerides induce metabolic shifts in dogs with idiopathic epilepsy: a multi-omics approach |
| Project Type: | Double blinded placebo-controlled dietary trial in dogs with idiopathic epilepsy |
| Project Summary: | Background: Medium chain triglycerides (MCT) are a class of dietary lipids with proposed beneficial health effects in animals and humans alike. Their mechanism of action in specific applications, like epilepsy, remains however unclear. Therefore, the aim of this study was to identify alterations in the fecal and plasma metabolome and fecal microbiome in dogs with idiopathic epilepsy (IE) following an MCT diet. A double-blinded placebo controlled dietary trial including 32 dogs with drug-resistant IE was established. Dogs were randomly allocated to an MCT (n = 16) or placebo diet (n = 16) for 3 months. Feces and plasma were collected at the start and end of the dietary trial. The metabolome of plasma and feces were analysed using liquid chromatography coupled to high resolution mass spectrometry, and the fecal bacterial phylogeny was examined using 16S rRNA sequencing. Fecal metabolome, plasma metabolome and fecal microbiome data were integrated using the DIABLO framework. Results: The MCT diet elicited distinct metabolic adaptations and modulated gut microbial composition, notably characterized by alterations in histidine and energy metabolism, a reduction in Escherichia-Shigella, and an enrichment of genera including Ruminococcus and Faecalitalea. Multiple correlations were observed between significantly altered microbial taxa and fecal metabolites like e.g. 1-methylhistidine and creatine. Conclusion: Our findings demonstrate that a diet rich in MCT does not only support peripheral energy metabolism, evidenced by stable carnitine metabolites and reduced plasma threonine, but also reshapes microbial composition. Therefore, potential involvement of the microbiota–gut–brain axis in the mechanism of action for MCT in dogs with IE was underscored. |
| Institute: | Ghent University |
| Department: | DI11 - DI04 |
| Laboratory: | ECAN - LIMET |
| Last Name: | Verdoodt |
| First Name: | Fien |
| Address: | Salisburylaan 133, Merelbeke, Flanders, 9820, Belgium |
| Email: | fien.verdoodt@ugent.be |
| Phone: | +32476076770 |
| Funding Source: | FWO |
| Publications: | Preprint available at https://doi.org/10.21203/rs.3.rs-8078706/v1 |
Summary of all studies in project PR002768
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST004369 | Medium chain triglycerides induce metabolic shifts in dogs with idiopathic epilepsy: a multi-omics approach - plasma metabolome | Canis lupus familiaris | Ghent University | MS | 2025-12-15 | 1 | 135 | Uploaded data (19.8G)* |
| ST004370 | Medium chain triglycerides induce metabolic shifts in dogs with idiopathic epilepsy: a multi-omics approach - fecal metabolome | Canis lupus familiaris | Ghent University | MS | 2025-12-15 | 1 | 134 | Uploaded data (19.1G)* |
