Summary of project PR002770
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002770. The data can be accessed directly via it's Project DOI: 10.21228/M8M83C This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR002770 |
| Project DOI: | doi: 10.21228/M8M83C |
| Project Title: | Assessing the effect of Aspirin treatment on Arachidonic acid levels in PIK3CA mutant and wild-type isogenic cells |
| Project Summary: | Previous work in our lab showed that PIK3CA mutant breast cancer have elevated levels of Arachidonic acid driven by activation of cPLA2. Follow-up work established cPLA2 as a target of Aspirin, thereby reducing Arachidonic acid levels as well as prostaglandin levels. Arachidonic Acid (AA) level was measured to confirm the impact of Aspirin treatment on its abundance downstream of wild-type (WT) or mutant (MUT) PIK3CA activity. Using Liquid Chromatography-Mass Spectrometry (LC-MS), immortalised breast MCF10A PIK3CA isogenic cells, mammary murine 4T1 PIK3CA isogenic cells and the common drug aspirin, we find that Arachidonic acid levels are reduced following treatment in the PIK3CA MUT context with no (MCF10A) or minor (4T1) effect in the WT context. |
| Institute: | The Institute of Cancer Research London |
| Department: | Cell and Molecular Biology |
| Laboratory: | Signalling and Cancer Metabolism |
| Last Name: | Poulogiannis |
| First Name: | George |
| Address: | 237 Fulham Road SW3 6JB LONDON |
| Email: | George.poulogiannis@icr.ac.uk |
| Phone: | +442071535347 |
Summary of all studies in project PR002770
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST004372 | Assessing the effect of Aspirin treatment on Arachidonic acid levels in PIK3CA mutant and wild-type isogenic cells | Homo sapiens, Mus musculus | The Institute of Cancer Research London | MS | 2025-12-15 | 1 | 38 | Uploaded data (7G)* |
