Summary of project PR002775
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002775. The data can be accessed directly via it's Project DOI: 10.21228/M8ZK26 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR002775 |
| Project DOI: | doi: 10.21228/M8ZK26 |
| Project Title: | Coordinated regulation of the metaboproteome by Hsp90 chaperones controls metabolic plasticity |
| Project Summary: | Heat shock protein 90 (Hsp90) chaperones participate in the stabilization and activation of hundreds of proteins, thereby acting as signaling hubs. A mitochondrial subpopulation of Hsp90 has been previously described; however, little is known about its role in metabolism. Here, we showed that loss of individual Hsp90 isoforms differentially affects oxygen consumption and metabolic flexibility. Proteomic and metabolomic evaluation demonstrated that Hsp90 regulates the mitochondrial metabolic network, including respiration, fatty acid oxidation, and redox homeostasis. Loss of the mitochondrial chaperone TRAP1 induced compensatory binding of Hsp90s to TRAP1-dependent proteins, indicating a mechanism for the role of Hsp90 chaperones in metabolic reprogramming. When considered with previous findings, a temporal pattern of regulation emerges whereby Hsp90s control the transcription, translation, import, and assembly of mitochondrial protein complexes. Our findings expand the scope of Hsp90-regulated processes and potentially inform the effects of isoform-specific Hsp90 inhibitors on metabolic reprogramming in cancer and other diseases. |
| Institute: | SUNY Upstate Medical University |
| Department: | Urology |
| Last Name: | Woodford |
| First Name: | Mark |
| Address: | 750 East Adams St, Syracuse, NY, 13210, USA |
| Email: | woodform@upstate.edu |
| Phone: | 3154646118 |
| Funding Source: | Intramural Pilot Program |
Summary of all studies in project PR002775
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST004377 | Metabolomic analysis of WT and TRAP1 KO HEK293 cells in the presence or absence of the mitochondrial Hsp90 inhibitor Gamitrinib-TPP. | Homo sapiens | SUNY Upstate Medical University | MS | 2025-12-01 | 1 | 12 | Uploaded data (559.2M)* |
| ST004378 | Metabolomic analysis of WT, Hsp90alpha KO or Hsp90beta KO HEK293 cells. | Homo sapiens | SUNY Upstate Medical University | MS | 2025-12-01 | 1 | 9 | Uploaded data (354.8M)* |
