Summary of project PR002777
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002777. The data can be accessed directly via it's Project DOI: 10.21228/M8Q26C This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR002777 |
| Project DOI: | doi: 10.21228/M8Q26C |
| Project Title: | Microbiome biogeography and ventral epithelial differences of rumen across different regions in three different ruminant species |
| Project Summary: | Ruminants thrive in diverse ecosystems by leveraging their rumen microbiome to ferment fibrous plants. However, the spatial biogeography of the rumen microbiome and genetic diversity of ventral rumen among the ruminants remain unknown. Here, we present a multi-omics integrating region-resolved microbiome across eleven ruminal sacs, metabolomics, and single-cell RNA sequencing, ATAC-seq, and bulk RNA-seq of ventral epithelium of roe deer, sika deer and sheep. Our results reveal species-specific rumen microbial compositions and metabolic capacities that contribute to differences in short-chain fatty acids and vitamin B production. We uncover functional divergence, genomic specialization and metabolic changes across distinct ruminal sacs microbiome. Single-cell profiling reveals changes of immune responses and structural remodeling in ventral rumen. We demonstrate that vitamin B12 promotes epithelial growth, and four novel genes that enhance stem cell differentiation. Our results highlight variation in microbial ecology and epithelial architecture among three ruminant species, offering insights to improve livestock productivity. |
| Institute: | Jilin Agricultural University |
| Department: | College of Animal Science and Technology |
| Laboratory: | Ruminant Nutrition Laboratory |
| Last Name: | Li |
| First Name: | Zhi Peng |
| Address: | No. 2888 Xincheng Street |
| Email: | zhplicaas@163.com |
| Phone: | 18043213681 |
Summary of all studies in project PR002777
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST004383 | GC-FID based metabolomic profiling of rumen samples across different regions in three ruminant species - investigation of short-chain fatty acids | Ovis aries, Capreolus pygargus, Cervus nippon | Jilin Agricultural University | MS | 2025-12-15 | 1 | 177 | Uploaded data (3.1M)* |
| ST004384 | Microbiome biogeography and ventral epithelial differences of rumen - vitamin profiling of rumen fluid from three ruminant species | Ovis aries, Capreolus pygargus, Cervus nippon | Jilin Agricultural University | MS | 2025-12-15 | 1 | 15 | Uploaded data (1M)* |
