Summary of project PR002779
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002779. The data can be accessed directly via it's Project DOI: 10.21228/M8FK2J This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR002779 |
| Project DOI: | doi: 10.21228/M8FK2J |
| Project Title: | PTPRD-based CAR T-cells demonstrate efficacy against IL-1RAP-positive AML |
| Project Type: | Research Study |
| Project Summary: | Acute myeloid leukemia (AML) remains a challenging disease to treat, primarily due to the persistence of leukemic stem cells (LSCs). Interleukin-1 receptor accessory protein (IL-1RAP) is overexpressed on the surface of LSCs across all AML subtypes, making it a compelling and promising marker compared to other common AML targets, as it is not expressed by normal hematopoietic stem cells. IL-1RAP interacts with various protein partners, but among them, only PTPRD (Protein Tyrosine Phosphatase Receptor Type D) has been shown to bind in Trans to IL-1RAP. We designed a CAR with the extracellular domain of PTPRD as the recognition domain. PTPRD CAR was cloned into lentiviral vector backbone containing the 4-1BB co-stimulatory domain. The efficacy of PTPRD CAR-T cell to eliminate specifically IL-1RAP+ cells was demonstrated by high intracellular TNFα expression and elevated IFNγ release, leading to significant cytotoxic effects compared to untransduced T cells in vitro. In a xenograft mouse model, PTPRD CAR-T cells exhibited potent anti-tumor activity, resulting in complete elimination of AML cells and prolonged survival of the mice. In conclusion, our preclinical findings suggest that PTPRD CAR-T cell therapy holds significant promise as a novel and effective strategy for AML treatment. |
| Institute: | King Faisal Specialist Hospital and Research Centre (KFSHRC) |
| Department: | Research and Innovation Centre |
| Laboratory: | Research Laboratories |
| Last Name: | Warda |
| First Name: | Walid |
| Address: | Makkah Al Mukarramah Rd, Al Mathar Ash Shamali, Riyadh 12713 |
| Email: | wwarda@kfshrc.edu.sa;walidwarda1990@gmail.com |
| Phone: | 966500858514 |
| Funding Source: | KFSH&RC Research and Innovation Fund |
| Contributors: | Walid Warda, Syed Osman Ahmed, Hanan Alqudairy, Ayadole Alaiya, Anas Abdel Rahman, Reem Almalki, Monther Alhamdoosh, Edward Hitti, Abdullah Aldhfyan, Ahmed Kotb, Nour Almozain, Falah Almohanna, Farhatullah Syed, Mahmoud Aljurf, Hazzaa Alzahrani and Jackie Y. Ying |
Summary of all studies in project PR002779
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST004386 | Metabolomic analysis of CAR T-cells and untransduced T-cells from healthy donor | Homo sapiens | King Faisal Specialist Hospital and Research Centre (KFSHRC) | MS* | 2025-12-15 | 1 | 6 | Uploaded data (24.1G)* |
