Summary of project PR002810
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002810. The data can be accessed directly via it's Project DOI: 10.21228/M8FC3V This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR002810 |
| Project DOI: | doi: 10.21228/M8FC3V |
| Project Title: | Modeling Lipid Homeostasis Using Stable Isotope Tracing and Flux Analysis |
| Project Summary: | Lipids represent the most diverse pool of metabolites found in cells, facilitating compartmentation, signaling, and other functions. Dysregulation of lipid metabolism is linked to disease states ranging from cancer to neurodegeneration. However, limited tools are available for quantifying metabolic fluxes across the lipidome. To directly measure reaction fluxes encompassing membrane lipid homeostasis, we apply stable isotope tracing, liquid chromatography-high-resolution mass spectrometry, and network-based mass isotopomer modeling to non-small cell lung cancer (NSCLC) models. Lipid metabolic flux analysis (MFA) enables the concurrent quantitation of fatty acid synthesis, elongation, headgroup assembly, and salvage reactions within virtually any biological system. Lipid-MFA highlights distinct changes in fatty acid synthase and very long-chain fatty acid (VLCFA) elongation fluxes in typical culture conditions. Using this approach, we resolve differences in sphingolipid recycling in p53-deficient versus liver kinase B1 (LKB1)-deficient NSCLC tumors using precision-cut lung slice culture. Finally, Lipid-MFA demonstrates the unique trafficking of long-chain versus very long-chain ceramide fluxes as well as the isozyme specificity of a classical ceramide synthase inhibitor. These results illustrate the ability of Lipid-MFA to quantify lipid homeostasis and elucidate molecular mechanisms in membrane lipid metabolism. |
| Institute: | Salk Insititute for Biological Studies |
| Last Name: | Wessendorf-Rodriguez |
| First Name: | Karl |
| Address: | 10010N Torrey Pines Rd, La Jolla, California, 92037, USA |
| Email: | kwessendorf@salk.edu |
| Phone: | 7874490440 |
Summary of all studies in project PR002810
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST004447 | Determine the impact of serum modulation to lipid homeostasis in a non-small cell lung cancer cell line | Homo sapiens | Salk Institute for Biological Studies | MS | 2025-12-22 | 1 | 18 | Uploaded data (1.7G)* |
| ST004448 | Determine the effect to lipid homeostasis of adding back functional LKB1 to an A549 cell line | Homo sapiens | Salk Institute for Biological Studies | MS | 2025-12-22 | 1 | 12 | Uploaded data (1.3G)* |
| ST004449 | Compare lipid homeostasis in two distinct non-small cell lung cancer cell lines | Homo sapiens | Salk Institute for Biological Studies | MS | 2025-12-22 | 1 | 12 | Uploaded data (633.6M)* |
| ST004450 | Determine the impact of an SMPD1 inhibitor on lipid homeostasis | Homo sapiens | Salk Institute for Biological Studies | MS | 2025-12-22 | 1 | 6 | Uploaded data (238.6M)* |
| ST004457 | Compare lipid metabolism in lung tumors from mice harboring Kras G12D/+ and either STK1-/- (KL) or Tp53 -/- mutations (KP). | Mus musculus | Salk Institute for Biological Studies | MS | 2025-12-22 | 1 | 48 | Uploaded data (1.6G)* |
| ST004458 | Determine the effect of a moderate dose of fumonisin B1 to the lipidome of non-small cell lung cancer cells | Homo sapiens | Salk Institute for Biological Studies | MS | 2025-12-22 | 1 | 12 | Uploaded data (457.5M)* |
| ST004460 | Determine the impact of a moderate concentrations of fumonisin B1 to sphingolipid abundances and CERS flux | Homo sapiens | Salk Institute for Biological Studies | MS | 2025-12-22 | 1 | 12 | Uploaded data (536.4M)* |
