Summary of Study ST000378
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000297. The data can be accessed directly via it's Project DOI: 10.21228/M8NP5K This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000378 |
| Study Title | 2 hydroxiglutarate with with IDH1-R132H inhibitor in neurospheres |
| Study Type | Regular |
| Study Summary | Tumor neurospheres carrying genetic lession NPI (NRAS, shP53, IDH1-R132H) colony 1 and 2 (C1 and C2); NPAI (NRAS, shP53, shATRX, IDH1-R132H) colony 1 and 2 (C1 and C2); and NPA (NRAS, shP53, shATRX) (C1 and C2). Was treated with IDH1-R132H inhibitor. By 2HG assay we want to check the effect of the inhibitor in our cells. |
| Institute | University of Michigan |
| Department | Biomedical Research Core Facilities |
| Laboratory | Metabolomics core |
| Last Name | Kachman |
| First Name | Maureen |
| Address | 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 |
| mkachman@med.umich.edu | |
| Phone | (734) 232-8175 |
| Submit Date | 2016-04-20 |
| Num Groups | 3 |
| Total Subjects | 12 |
| Study Comments | (shATRX) : ATRX knockdown sequence into a Sleeping Beauty (SB) transposase-responsive plasmid |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | LC-MS |
| Release Date | 2016-12-22 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000297 |
| Project DOI: | doi: 10.21228/M8NP5K |
| Project Title: | IDH1(R132H) mutations activity in neurospheres |
| Project Summary: | assay 2HG procution in neurospheres expressing IDH1 mutated |
| Institute: | University of Michigan |
| Department: | Neurosurgery |
| Laboratory: | Castro Lab |
| Last Name: | Castro |
| First Name: | Maria |
| Address: | Ann Arbor, MI |
| Email: | mariacas@umich.edu |
| Phone: | 734-764-0850 |
Subject:
| Subject ID: | SU000399 |
| Subject Type: | Animal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Species Group: | Mammal |
Factors:
Subject type: Animal; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | shATRX plasmid |
|---|---|---|
| SA017061 | S00018624 | no | IDH1-R132H mutation:no |
| SA017062 | S00018487 | no | IDH1-R132H mutation:yes |
| SA017063 | S00018488 | no | IDH1-R132H mutation:yes |
| SA017064 | S00018625 | no | IDH1-R132H mutation:yes |
| SA017065 | S00018489 | no | IDH1-R132H mutation:yes |
| SA017066 | S00018490 | no | IDH1-R132H mutation:yes |
| SA017067 | S00018620 | yes | IDH1-R132H mutation:no |
| SA017068 | S00018622 | yes | IDH1-R132H mutation:no |
| SA017069 | S00018498 | yes | IDH1-R132H mutation:no |
| SA017070 | S00018496 | yes | IDH1-R132H mutation:no |
| SA017071 | S00018497 | yes | IDH1-R132H mutation:no |
| SA017072 | S00018495 | yes | IDH1-R132H mutation:no |
| SA017073 | S00018492 | yes | IDH1-R132H mutation:yes |
| SA017074 | S00018491 | yes | IDH1-R132H mutation:yes |
| SA017075 | S00018493 | yes | IDH1-R132H mutation:yes |
| SA017076 | S00018494 | yes | IDH1-R132H mutation:yes |
| SA017077 | S00018621 | yes | IDH1-R132H mutation:yes |
| SA017078 | S00018623 | yes | IDH1-R132H mutation:yes |
| Showing results 1 to 18 of 18 |
Collection:
| Collection ID: | CO000393 |
| Collection Summary: | none |
| Sample Type: | Cell Line, Tumor |
Treatment:
| Treatment ID: | TR000413 |
| Treatment Summary: | none |
Sample Preparation:
| Sampleprep ID: | SP000406 |
| Sampleprep Summary: | none |
| Sampleprep Protocol Filename: | 15DEC2015_2HG_cells_Castro.docx |
Combined analysis:
| Analysis ID | AN000612 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | None |
| Column | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | Agilent 6410A QQQ |
| Ion Mode | NEGATIVE |
| Units | µmol/g |
Chromatography:
| Chromatography ID: | CH000438 |
| Chromatography Summary: | none |
| Methods ID: | DQM0033 |
| Methods Filename: | 2015-04-07-2HG.m.zip |
| Instrument Name: | None |
| Column Name: | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS000545 |
| Analysis ID: | AN000612 |
| Instrument Name: | Agilent 6410A QQQ |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
| Analysis Protocol File: | EX00452-2HG-QUANT-method.m.zip |
