Summary of Study ST000824

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000586. The data can be accessed directly via it's Project DOI: 10.21228/M8XD4X This work is supported by NIH grant, U2C- DK119886.

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Study IDST000824
Study Title2HG concentration in human IDH1m cells
Study TypeMS analysis
Study SummarySJGBM2 and MGG8 glioma cells were stable transfected with IDH1-R132H mutated. The expression of IDH1-R132H was confirmed by Western Blot assay. The aim of this project is analyze the production of 2HG in the stable transfected cells (SJGBM-IDH1m and MGG8-IDH1m) compared with the control group WT.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2017-07-14
Num Groups4
Total Subjects1
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2019-01-22
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8XD4X
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000586
Project DOI:doi: 10.21228/M8XD4X
Project Title:Production of 2HG of human glioma cells
Project Type:MS analysis
Project Summary:Evaluate the concentration of 2HG in human glioma cells harboring IDH1-R132H mutation
Institute:University of Michigan
Department:Neurosurgery
Laboratory:Castro Lab
Last Name:Castro
First Name:Maria
Address:Ann Arbor, MI
Email:mariacas@umich.edu
Phone:734-764-0850

Subject:

Subject ID:SU000850
Subject Type:HUMAN
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Human

Factors:

Subject type: HUMAN; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Cell line
SA045855S00028190MGG8 | IDH1-R132H EXPRESSION:NO | IDH1-R132H INHIBITOR:NO
SA045856S00028188MGG8 | IDH1-R132H EXPRESSION:NO | IDH1-R132H INHIBITOR:NO
SA045857S00028189MGG8 | IDH1-R132H EXPRESSION:NO | IDH1-R132H INHIBITOR:NO
SA045858S00028202MGG8 | IDH1-R132H EXPRESSION:NO | IDH1-R132H INHIBITOR:YES
SA045859S00028201MGG8 | IDH1-R132H EXPRESSION:NO | IDH1-R132H INHIBITOR:YES
SA045860S00028200MGG8 | IDH1-R132H EXPRESSION:NO | IDH1-R132H INHIBITOR:YES
SA045861S00028186MGG8 | IDH1-R132H EXPRESSION:YES | IDH1-R132H INHIBITOR:NO
SA045862S00028185MGG8 | IDH1-R132H EXPRESSION:YES | IDH1-R132H INHIBITOR:NO
SA045863S00028187MGG8 | IDH1-R132H EXPRESSION:YES | IDH1-R132H INHIBITOR:NO
SA045864S00028199MGG8 | IDH1-R132H EXPRESSION:YES | IDH1-R132H INHIBITOR:YES
SA045865S00028198MGG8 | IDH1-R132H EXPRESSION:YES | IDH1-R132H INHIBITOR:YES
SA045866S00028197MGG8 | IDH1-R132H EXPRESSION:YES | IDH1-R132H INHIBITOR:YES
SA045867S00028184SJGBM2 | IDH1-R132H EXPRESSION:NO | IDH1-R132H INHIBITOR:NO
SA045868S00028183SJGBM2 | IDH1-R132H EXPRESSION:NO | IDH1-R132H INHIBITOR:NO
SA045869S00028182SJGBM2 | IDH1-R132H EXPRESSION:NO | IDH1-R132H INHIBITOR:NO
SA045870S00028196SJGBM2 | IDH1-R132H EXPRESSION:NO | IDH1-R132H INHIBITOR:YES
SA045871S00028195SJGBM2 | IDH1-R132H EXPRESSION:NO | IDH1-R132H INHIBITOR:YES
SA045872S00028194SJGBM2 | IDH1-R132H EXPRESSION:NO | IDH1-R132H INHIBITOR:YES
SA045873S00028179SJGBM2 | IDH1-R132H EXPRESSION:YES | IDH1-R132H INHIBITOR:NO
SA045874S00028181SJGBM2 | IDH1-R132H EXPRESSION:YES | IDH1-R132H INHIBITOR:NO
SA045875S00028180SJGBM2 | IDH1-R132H EXPRESSION:YES | IDH1-R132H INHIBITOR:NO
SA045876S00028191SJGBM2 | IDH1-R132H EXPRESSION:YES | IDH1-R132H INHIBITOR:YES
SA045877S00028192SJGBM2 | IDH1-R132H EXPRESSION:YES | IDH1-R132H INHIBITOR:YES
SA045878S00028193SJGBM2 | IDH1-R132H EXPRESSION:YES | IDH1-R132H INHIBITOR:YES
Showing results 1 to 24 of 24

Collection:

Collection ID:CO000844
Collection Summary:-
Sample Type:Glioma cells

Treatment:

Treatment ID:TR000864
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP000857
Sampleprep Summary:-
Sampleprep Protocol Filename:A037-2HG_cells_updated-20160721.pdf

Combined analysis:

Analysis ID AN001310
Analysis type MS
Chromatography type HILIC
Chromatography system Agilent
Column Phenomenex Luna NH2 (150 x 1mm,3um)
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name Agilent 6490A QQQ
Ion Mode NEGATIVE
Units uM/ng protein

Chromatography:

Chromatography ID:CH000917
Instrument Name:Agilent
Column Name:Phenomenex Luna NH2 (150 x 1mm,3um)
Solvent A:100% water; 2mM ammonium formate, pH 3.3-4.01,adjust with formic acid
Solvent B:100% acetonitrile; 0.1% formic acid
Chromatography Type:HILIC

MS:

MS ID:MS001203
Analysis ID:AN001310
Instrument Name:Agilent 6490A QQQ
Instrument Type:Triple quadrupole
MS Type:ESI
Ion Mode:NEGATIVE
Analysis Protocol File:A037-2HG_cells_updated-20160721.pdf
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