Summary of Study ST003203

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001997. The data can be accessed directly via it's Project DOI: 10.21228/M8KT6Q This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Study ID	ST003203
Study Title	Metabolomic investigation of tear fluid biomarkers obtained from participants enrolled in the BiZiFED nutrition study
Study Summary	Zinc is an essential micronutrient, and its deficiency is particularly prevalent amongst the female population of Pakistan, leading to a substantial health burden on women of reproductive age and their children. Biofortification of wheat with zinc may offer a cost-effective strategy to attempt to improve nutritional status on a national scale. Biofortification with Zinc and Iron for Eliminating Deficiency (BiZiFED) is research program that aims to evaluate the impact of consuming zinc biofortified wheat flour on the zinc status and associated health outcomes of vulnerable communities northwest Pakistan. Of the health outcomes measured, tear biofluid was collected as a non-invasive method of determining whether zinc supplementation altered tear metabolomic biomarkers. In a crossover trial, 25 women of reproductive age received standard, non-biofortified wheat flour, and another 25 women received zinc-biofortified wheat flour for 8 weeks. At the end of this period, the nutritional intervention was switched for a further 8 weeks, whereby those individuals receiving the zinc-biofortified flour subsequently received the standard flour, whilst those receiving standard flour subsequently received the zinc-biofortified flour. The tear biofluid was collected using Schirmer strips after a 2-week baseline period and after 8 weeks and 16 weeks of the study. Metabolomic analysis was conducted using the MxP Quant 500 kit (Biocrates). Following multiple comparisons, four metabolites were significantly correlated with plasma zinc concentration, two demonstrating a positive correlation and two demonstrating a negative correlation. Five metabolites were significantly altered in response to zinc supplementation with biofortified wheat flour. These results highlight the potential of using tear biofluids as an alternative source for metabolomic biomarkers for the assessment of zinc status of individuals enrolled in nutritional studies.
Institute	Institute for Global Food Security
Last Name	Pan
First Name	Xiaobei
Address	19 Chlorine Gardens, Belfast, Antrim, BT9 5DL, United Kingdom
Email	x.pan@qub.ac.uk
Phone	02890975984
Submit Date	2024-04-22
Raw Data Available	Yes
Raw Data File Type(s)	wiff
Analysis Type Detail	LC-MS
Release Date	2024-05-31
Release Version	1

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Project:

Project ID:	PR001997
Project DOI:	doi: 10.21228/M8KT6Q
Project Title:	Metabolomic investigation of tear fluid biomarkers
Project Summary:	This project aims to evaluate the impact of consuming zinc biofortified wheat flour on the zinc status and associated health outcomes of vulnerable communities northwest Pakistan. Tear biofluid was collected as a non-invasive method of determining whether zinc supplementation altered tear metabolomic biomarkers.
Institute:	Institute for Global Food Security
Last Name:	Pan
First Name:	Xiaobei
Address:	19 Chlorine Gardens, Belfast, Antrim, BT9 5DL, United Kingdom
Email:	x.pan@qub.ac.uk
Phone:	02890975984

Subject:

Subject ID:	SU003322
Subject Type:	Human
Subject Species:	Homo sapiens
Taxonomy ID:	9606
Gender:	Male and female

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id	local_sample_id	Sample source	Study Arm	Timepoint	PZC (< 660 ug/L)
SA348706	BZ2F35	Tear	1	Week 10	N
SA348707	BZ2F15	Tear	1	Week 10	N
SA348708	BZ2F19	Tear	1	Week 10	N
SA348709	BZ2F37	Tear	1	Week 10	N
SA348710	BZ2F1	Tear	1	Week 10	N
SA348711	BZ2F47	Tear	1	Week 10	N
SA348712	BZ2F3	Tear	1	Week 10	N
SA348713	BZ2F11	Tear	1	Week 10	Y
SA348714	BZ2F17	Tear	1	Week 10	Y
SA348715	BZ2F33	Tear	1	Week 10	Y
SA348716	BZ2F29	Tear	1	Week 10	Y
SA348717	BZ2F45	Tear	1	Week 10	Y
SA348718	BZ2F23	Tear	1	Week 10	Y
SA348719	BZ3F3	Tear	1	Week 18	N
SA348720	BZ3F15	Tear	1	Week 18	N
SA348721	BZ3F19	Tear	1	Week 18	N
SA348722	BZ3F1	Tear	1	Week 18	N
SA348723	BZ3F35	Tear	1	Week 18	N
SA348724	BZ3F37	Tear	1	Week 18	N
SA348725	BZ3F47	Tear	1	Week 18	N
SA348726	BZ3F17	Tear	1	Week 18	Y
SA348727	BZ3F11	Tear	1	Week 18	Y
SA348728	BZ3F23	Tear	1	Week 18	Y
SA348729	BZ3F45	Tear	1	Week 18	Y
SA348730	BZ3F33	Tear	1	Week 18	Y
SA348731	BZ3F29	Tear	1	Week 18	Y
SA348732	BZ1F3	Tear	1	Week 2	N
SA348733	BZ1F15	Tear	1	Week 2	N
SA348734	BZ1F1	Tear	1	Week 2	N
SA348735	BZ1F35	Tear	1	Week 2	N
SA348736	BZ1F19	Tear	1	Week 2	N
SA348737	BZ1F37	Tear	1	Week 2	N
SA348738	BZ1F47	Tear	1	Week 2	N
SA348739	BZ1F33	Tear	1	Week 2	Y
SA348740	BZ1F11	Tear	1	Week 2	Y
SA348741	BZ1F45	Tear	1	Week 2	Y
SA348742	BZ1F23	Tear	1	Week 2	Y
SA348743	BZ1F29	Tear	1	Week 2	Y
SA348744	BZ1F17	Tear	1	Week 2	Y
SA348745	BZ2F22	Tear	2	Week 10	N
SA348746	BZ2F24	Tear	2	Week 10	N
SA348747	BZ2F26	Tear	2	Week 10	N
SA348748	BZ2F18	Tear	2	Week 10	N
SA348749	BZ2F30	Tear	2	Week 10	N
SA348750	BZ2F42	Tear	2	Week 10	N
SA348751	BZ2F10	Tear	2	Week 10	N
SA348752	BZ2F38	Tear	2	Week 10	N
SA348753	BZ2F16	Tear	2	Week 10	N
SA348754	BZ2F4	Tear	2	Week 10	Y
SA348755	BZ2F6	Tear	2	Week 10	Y
SA348756	BZ2F14	Tear	2	Week 10	Y
SA348757	BZ2F8	Tear	2	Week 10	Y
SA348758	BZ3F10	Tear	2	Week 18	N
SA348759	BZ3F38	Tear	2	Week 18	N
SA348760	BZ3F42	Tear	2	Week 18	N
SA348761	BZ3F16	Tear	2	Week 18	N
SA348762	BZ3F18	Tear	2	Week 18	N
SA348763	BZ3F24	Tear	2	Week 18	N
SA348764	BZ3F30	Tear	2	Week 18	N
SA348765	BZ3F22	Tear	2	Week 18	N
SA348766	BZ3F26	Tear	2	Week 18	N
SA348767	BZ3F4	Tear	2	Week 18	Y
SA348768	BZ3F8	Tear	2	Week 18	Y
SA348769	BZ3F14	Tear	2	Week 18	Y
SA348770	BZ3F6	Tear	2	Week 18	Y
SA348771	BZ1F10	Tear	2	Week 2	N
SA348772	BZ1F30	Tear	2	Week 2	N
SA348773	BZ1F38	Tear	2	Week 2	N
SA348774	BZ1F42	Tear	2	Week 2	N
SA348775	BZ1F26	Tear	2	Week 2	N
SA348776	BZ1F24	Tear	2	Week 2	N
SA348777	BZ1F16	Tear	2	Week 2	N
SA348778	BZ1F18	Tear	2	Week 2	N
SA348779	BZ1F22	Tear	2	Week 2	N
SA348780	BZ1F4	Tear	2	Week 2	Y
SA348781	BZ1F6	Tear	2	Week 2	Y
SA348782	BZ1F14	Tear	2	Week 2	Y
SA348783	BZ1F8	Tear	2	Week 2	Y

Showing results 1 to 78 of 78

Showing results 1 to 78 of 78

Collection:

Collection ID:	CO003315
Collection Summary:	Tears were collected on Schirmer tear test strips (4701001, Haag-Streit UK Ltd., Essex, UK) from both eyes of the study participants. Briefly, the Schirmer strips were folded at the notch, before resting the rounded notch in the inferior conjunctival fornix (inside the lower eyelid) (FIGURE SUPPLEMENTARY 1A). Participants were given the option to close their eyes. The Schirmer strips were removed when the strips became saturated with tear fluid, or once 5 minutes had passed, whichever came first. The strips were shipped to QUB at ambient temperature, and stored at -80°C prior to analysis .
Sample Type:	Tear

Treatment:

Treatment ID:	TR003331
Treatment Summary:	In brief, a total of 50 households, with at least one 16-49 year-old woman, who was neither pregnant nor breastfeeding nor consuming additional nutritional supplementation, were randomly selected for the study. The selected households were provided with either freshly-milled Zincol-2016/NR-421 grain (genetically and agronomically Zn-biofortified) or Galaxy-2013 grain (control), with the intention that these were the only flours to be consumed for the duration of the study. A two-week baseline period was established with control flour, before the households were randomly separated into two groups for the purposes of the study, one which received Zincol-2016/NR-421 grain for eight weeks and then Galaxy-2013 control grain for eight weeks, whilst the other received control grain for eight weeks followed by Zn-biofortified grain. Where possible, blood and tear samples were collected at the 2-week (baseline), 10-week and 18-week time points of the study. Then tear samples from study participants were selected based on the zinc status of the individual at the baseline timepoint. Plasma zinc concentration is the most commonly used marker of zinc status and a value of 660 µgZn/L was used as the threshold below which a participant was categorized as zinc deficient.

Treatment ID:

TR003331

Treatment Summary:

In brief, a total of 50 households, with at least one 16-49 year-old woman, who was neither pregnant nor breastfeeding nor consuming additional nutritional supplementation, were randomly selected for the study. The selected households were provided with either freshly-milled Zincol-2016/NR-421 grain (genetically and agronomically Zn-biofortified) or Galaxy-2013 grain (control), with the intention that these were the only flours to be consumed for the duration of the study. A two-week baseline period was established with control flour, before the households were randomly separated into two groups for the purposes of the study, one which received Zincol-2016/NR-421 grain for eight weeks and then Galaxy-2013 control grain for eight weeks, whilst the other received control grain for eight weeks followed by Zn-biofortified grain. Where possible, blood and tear samples were collected at the 2-week (baseline), 10-week and 18-week time points of the study. Then tear samples from study participants were selected based on the zinc status of the individual at the baseline timepoint. Plasma zinc concentration is the most commonly used marker of zinc status and a value of 660 µgZn/L was used as the threshold below which a participant was categorized as zinc deficient.

Sample Preparation:

Sampleprep ID:	SP003329
Sampleprep Summary:	One Schirmer strip from each participant at each timepoint was cut into 5 mm sections, up until the tear front was reached, and placed into sterile tubes. A length-adjusted volume of ice-cold 80% methanol was then added to each tube (500 µL per 46 mm Schirmer strip). Strips were vigorously vortexed in the extraction solution for 2 mins before centrifugation at 15,700 RCF at 4°C. Supernatant was transferred to a fresh, clean tube and stored at -80°C before analysis. Targeted metabolomics profiling was performed using a commercially available kit, MxP® Quant500 kit as instructed.

Sampleprep ID:

SP003329

Sampleprep Summary:

One Schirmer strip from each participant at each timepoint was cut into 5 mm sections, up until the tear front was reached, and placed into sterile tubes. A length-adjusted volume of ice-cold 80% methanol was then added to each tube (500 µL per 46 mm Schirmer strip). Strips were vigorously vortexed in the extraction solution for 2 mins before centrifugation at 15,700 RCF at 4°C. Supernatant was transferred to a fresh, clean tube and stored at -80°C before analysis. Targeted metabolomics profiling was performed using a commercially available kit, MxP® Quant500 kit as instructed.

Combined analysis:

Analysis ID	AN005253	AN005254
Analysis type	MS	MS
Chromatography type	Reversed phase	Reversed phase
Chromatography system	AB Sciex5500+	AB Sciex5500+
Column	Biocrates MxP Quant500 (XL) Kit column system	Biocrates MxP Quant500 (XL) Kit column system
MS Type	ESI	ESI
MS instrument type	Triple quadrupole	Triple quadrupole
MS instrument name	ABI Sciex 5500+	ABI Sciex 5500+
Ion Mode	POSITIVE	NEGATIVE
Units	uM	uM

Chromatography:

Chromatography ID:	CH003977
Chromatography Summary:	Biocrates MxP Quant500 kit column, Product number: 21117.2 (https://shop.biocrates.com/21117.2)
Instrument Name:	AB Sciex5500+
Column Name:	Biocrates MxP Quant500 (XL) Kit column system
Column Temperature:	50
Flow Gradient:	0.8ml/min: 0-0.25min: 0%B, 0.25-1.5min: 12%B, 1.5-2.7min: 17.5%B, 2.7-4min: 50%B, 4-4.5min: 100%B, 1ml/min: 4.5-4.7min: 100%B, 4.7-5.0min: 100%B, 5.0-5.1min: 100%B, 0.8ml/min: 5.1-5.8min: 100%B
Flow Rate:	0.8-1ml/min
Solvent A:	100% Water; 0.2% formic acid
Solvent B:	100% Acetonitrile; 0.2% formic acid
Chromatography Type:	Reversed phase

MS:

MS ID:	MS004985
Analysis ID:	AN005253
Instrument Name:	ABI Sciex 5500+
Instrument Type:	Triple quadrupole
MS Type:	ESI
MS Comments:	ESI positive acquisition. Data were acquired using Analyst and processed using the MetIDQ software.
Ion Mode:	POSITIVE

MS ID:	MS004986
Analysis ID:	AN005254
Instrument Name:	ABI Sciex 5500+
Instrument Type:	Triple quadrupole
MS Type:	ESI
MS Comments:	ESI negative acquisition. Data were acquired using Analyst and processed using the MetIDQ software.
Ion Mode:	NEGATIVE