Summary of Study ST000758

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000547. The data can be accessed directly via it's Project DOI: 10.21228/M8ZH48 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST000758
Study TitleEffects of caloric restriction in HCR/LCR rats
Study TypeMS analysis
Study SummaryOne year caloric restriction (CR) compared to ad lib (AL) feeding on metabolic and clinical parameters to assess association with intrinsic oxidative capacity. High and low capacity running rats (HCR and LCR) were randomized to ad lib feeding vs. 40% caloric restriction starting at 8 weeks of age. Experiment was continued for 52 weeks when blood and tissue were collected. Exercise capacity, CLAMS assessment of fuel utilization and clinical parameters were determined at 8 weeks, 8 months and 12 months of age. Tissue was collected in the fed (2 hours after re-feeding) or fasted stated all groups.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2017-06-19
Num Groups7
Total Subjects5
Raw Data AvailableYes
Raw Data File Type(s)wiff
Analysis Type DetailLC-MS
Release Date2017-12-06
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8ZH48
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000547
Project DOI:doi: 10.21228/M8ZH48
Project Title:Caloric Restriction and Oxidative capactiy in rats
Project Type:MS analysis
Project Summary:One year caloric restriction compared to ad lib feeding on metabolic and clinical parameters to assess association with intrinsic oxidative capacity
Institute:University of Michigan
Department:Internal Medicine
Laboratory:Burant Lab
Last Name:Burant
First Name:Chuck
Address:1500 E. Medical Center Drive Ann Arbor, MI 48109
Email:burantc@umich.edu
Phone:734 615-3481

Subject:

Subject ID:SU000781
Subject Type:Rat
Subject Species:Rattus norvegicus
Taxonomy ID:10116
Species Group:Mammal

Factors:

Subject type: Rat; Subject species: Rattus norvegicus (Factor headings shown in green)

mb_sample_id local_sample_id Feeding
SA041612S00018548Fast | Group:HCR-AL
SA041613S00018549Fast | Group:HCR-AL
SA041614S00018550Fast | Group:HCR-AL
SA041615S00018551Fast | Group:HCR-AL
SA041616S00018552Fast | Group:HCR-AL
SA041617S00018571Fast | Group:HCR-CR
SA041618S00018570Fast | Group:HCR-CR
SA041619S00018573Fast | Group:HCR-CR
SA041620S00018572Fast | Group:HCR-CR
SA041621S00018574Fast | Group:HCR-CR
SA041622S00018575Fast | Group:HCR-CR
SA041623S00018538Fast | Group:LCR-AL
SA041624S00018537Fast | Group:LCR-AL
SA041625S00018536Fast | Group:LCR-AL
SA041626S00018539Fast | Group:LCR-AL
SA041627S00018540Fast | Group:LCR-AL
SA041628S00018541Fast | Group:LCR-AL
SA041629S00018560Fast | Group:LCR-CR
SA041630S00018561Fast | Group:LCR-CR
SA041631S00018563Fast | Group:LCR-CR
SA041632S00018562Fast | Group:LCR-CR
SA041633S00018559Fast | Group:LCR-CR
SA041634S00018558Fast | Group:LCR-CR
SA041635S00018553Fed | Group:HCR-AL
SA041636S00018555Fed | Group:HCR-AL
SA041637S00018554Fed | Group:HCR-AL
SA041638S00018557Fed | Group:HCR-AL
SA041639S00018556Fed | Group:HCR-AL
SA041640S00018577Fed | Group:HCR-CR
SA041641S00018576Fed | Group:HCR-CR
SA041642S00018578Fed | Group:HCR-CR
SA041643S00018580Fed | Group:HCR-CR
SA041644S00018579Fed | Group:HCR-CR
SA041645S00018581Fed | Group:HCR-CR
SA041646S00018543Fed | Group:LCR-AL
SA041647S00018542Fed | Group:LCR-AL
SA041648S00018544Fed | Group:LCR-AL
SA041649S00018547Fed | Group:LCR-AL
SA041650S00018545Fed | Group:LCR-AL
SA041651S00018546Fed | Group:LCR-AL
SA041652S00018565Fed | Group:LCR-CR
SA041653S00018564Fed | Group:LCR-CR
SA041654S00018566Fed | Group:LCR-CR
SA041655S00018567Fed | Group:LCR-CR
SA041656S00018568Fed | Group:LCR-CR
SA041657S00018569Fed | Group:LCR-CR
SA041658S00020152Pooled plasma | Group:Pooled plasma
Showing results 1 to 47 of 47

Collection:

Collection ID:CO000775
Collection Summary:-

Treatment:

Treatment ID:TR000795
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP000788
Sampleprep Summary:-
Sampleprep Protocol Filename:A003_-_Untargeted_Metabolomics.pdf

Combined analysis:

Analysis ID AN001190 AN001191 AN001192 AN001193
Analysis type MS MS MS MS
Chromatography type Reversed phase Reversed phase Reversed phase Reversed phase
Chromatography system Agilent Agilent Shimadzu CTO-20A Nexera X2 Shimadzu CTO-20A Nexera X2
Column Waters Acquity HSS T3 (50 x 2.1mm,1.8um) Waters Acquity HSS T3 (50 x 2.1mm,1.8um) Waters Acquity HSS T3 (50 x 2.1mm,1.8um) Waters Acquity HSS T3 (50 x 2.1mm,1.8um)
MS Type ESI ESI ESI ESI
MS instrument type QTOF QTOF Triple TOF Triple TOF
MS instrument name Agilent 6530 QTOF Agilent 6530 QTOF ABI Sciex 5600+ TripleTOF ABI Sciex 5600+ TripleTOF
Ion Mode NEGATIVE POSITIVE NEGATIVE POSITIVE
Units peak area peak area peak area normalized peak area normalized

Chromatography:

Chromatography ID:CH000835
Instrument Name:Agilent
Column Name:Waters Acquity HSS T3 (50 x 2.1mm,1.8um)
Chromatography Type:Reversed phase
  
Chromatography ID:CH000836
Instrument Name:Shimadzu CTO-20A Nexera X2
Column Name:Waters Acquity HSS T3 (50 x 2.1mm,1.8um)
Column Temperature:55
Flow Rate:0.400uL/min
Solvent A:40% acetonitrile/60% water; 10 mM ammonium acetate
Solvent B:10% acetonitrile/5% water/85% isopropanol; 10 mM ammonium acetate
Chromatography Type:Reversed phase

MS:

MS ID:MS001085
Analysis ID:AN001190
Instrument Name:Agilent 6530 QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:NEGATIVE
Analysis Protocol File:A003_-_Untargeted_Metabolomics.pdf
  
MS ID:MS001086
Analysis ID:AN001191
Instrument Name:Agilent 6530 QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:POSITIVE
Analysis Protocol File:A003_-_Untargeted_Metabolomics.pdf
  
MS ID:MS001087
Analysis ID:AN001192
Instrument Name:ABI Sciex 5600+ TripleTOF
Instrument Type:Triple TOF
MS Type:ESI
Ion Mode:NEGATIVE
Analysis Protocol File:A004_-_Shotgun_Lipidomics.pdf
  
MS ID:MS001088
Analysis ID:AN001193
Instrument Name:ABI Sciex 5600+ TripleTOF
Instrument Type:Triple TOF
MS Type:ESI
Ion Mode:POSITIVE
Analysis Protocol File:A004_-_Shotgun_Lipidomics.pdf
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