Summary of Study ST000005

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000004. The data can be accessed directly via it's Project DOI: 10.21228/M8MW26 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

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Study IDST000005
Study TitleTimecourse on RAW 264.7 cells treated with Kdo2-Lipid A and compactin
Study TypeTimecourse experiment
Study SummaryLipidomics studies on macrophages - RAW 264.7 cells treated with Kdo2-Lipid A and compactin. Experiments were conducted with RAW264.7 cells fed 10% fetal calf serum. 8-timepoint study: Measurements were taken at 0, 0.5,1,2,4,8, 12, and 24hrs for: (i) compactin, (ii) Kdo2-Lipid A, (iii) compactin + Kdo2-Lipid A. and (iv) control
Institute
LIPID MAPS
DepartmentMultiple
LaboratoryMultiple
Last NameFahy
First NameEoin
Emailefahy@ucsd.edu
Submit Date2013-02-20
PublicationsDennis, E.A. et al A mouse macrophage lipidome. J. Biol. Chem., 285, 39976-39985 (2010).
Raw Data AvailableNo
Analysis Type DetailGC-MS/LC-MS
Release Date2013-03-22
Release Version1
Eoin Fahy Eoin Fahy
https://dx.doi.org/10.21228/M8MW26
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000004
Project DOI:doi: 10.21228/M8MW26
Project Title:LIPID MAPS Lipidomics studies
Project Type:MS quantitative analysis
Project Summary:Multi-center quantitative lipidomics studies on samples from human and murine sources (LIPIDMAPS)
Institute:LIPID MAPS
Department:Multiple
Laboratory:Multiple
Last Name:Fahy
First Name:Eoin
Address:UCSD
Email:efahy@ucsd.edu
Funding Source:NIGMS

Subject:

Subject ID:SU000006
Subject Type:Animal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:RAW 264.7
Species Group:Mammal

Factors:

Subject type: Animal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Hours Compactin KLA
SA054136KS0130.5 50uM 100ng/ml
SA054137KS0140.5 50uM 100ng/ml
SA054138KS0150.5 50uM 100ng/ml
SA054139KS0160.5 50uM 100ng/ml
SA054128KS0050.5 50uM None
SA054129KS0060.5 50uM None
SA054130KS0070.5 50uM None
SA054131KS0080.5 50uM None
SA054132KS0090.5 None 100ng/ml
SA054133KS0100.5 None 100ng/ml
SA054134KS0110.5 None 100ng/ml
SA054135KS0120.5 None 100ng/ml
SA054124KS0010.5 None None
SA054125KS0020.5 None None
SA054126KS0030.5 None None
SA054127KS0040.5 None None
SA054140KS0170 None None
SA054141KS0180 None None
SA054142KS0190 None None
SA054143KS0200 None None
SA054172KS04912 50uM 100ng/ml
SA054173KS05012 50uM 100ng/ml
SA054174KS05112 50uM 100ng/ml
SA054175KS05212 50uM 100ng/ml
SA054164KS04112 50uM None
SA054165KS04212 50uM None
SA054166KS04312 50uM None
SA054167KS04412 50uM None
SA054168KS04512 None 100ng/ml
SA054169KS04612 None 100ng/ml
SA054170KS04712 None 100ng/ml
SA054171KS04812 None 100ng/ml
SA054160KS03712 None None
SA054161KS03812 None None
SA054162KS03912 None None
SA054163KS04012 None None
SA054156KS0331 50uM 100ng/ml
SA054157KS0341 50uM 100ng/ml
SA054158KS0351 50uM 100ng/ml
SA054159KS0361 50uM 100ng/ml
SA054148KS0251 50uM None
SA054149KS0261 50uM None
SA054150KS0271 50uM None
SA054151KS0281 50uM None
SA054152KS0291 None 100ng/ml
SA054153KS0301 None 100ng/ml
SA054154KS0311 None 100ng/ml
SA054155KS0321 None 100ng/ml
SA054144KS0211 None None
SA054145KS0221 None None
SA054146KS0231 None None
SA054147KS0241 None None
SA054204KS08124 50uM 100ng/ml
SA054205KS08224 50uM 100ng/ml
SA054206KS08324 50uM 100ng/ml
SA054207KS08424 50uM 100ng/ml
SA054196KS07324 50uM None
SA054197KS07424 50uM None
SA054198KS07524 50uM None
SA054199KS07624 50uM None
SA054200KS07724 None 100ng/ml
SA054201KS07824 None 100ng/ml
SA054202KS07924 None 100ng/ml
SA054203KS08024 None 100ng/ml
SA054192KS06924 None None
SA054193KS07024 None None
SA054194KS07124 None None
SA054195KS07224 None None
SA054188KS0652 50uM 100ng/ml
SA054189KS0662 50uM 100ng/ml
SA054190KS0672 50uM 100ng/ml
SA054191KS0682 50uM 100ng/ml
SA054180KS0572 50uM None
SA054181KS0582 50uM None
SA054182KS0592 50uM None
SA054183KS0602 50uM None
SA054184KS0612 None 100ng/ml
SA054185KS0622 None 100ng/ml
SA054186KS0632 None 100ng/ml
SA054187KS0642 None 100ng/ml
SA054176KS0532 None None
SA054177KS0542 None None
SA054178KS0552 None None
SA054179KS0562 None None
SA054220KS0974 50uM 100ng/ml
SA054221KS0984 50uM 100ng/ml
SA054222KS0994 50uM 100ng/ml
SA054223KS1004 50uM 100ng/ml
SA054212KS0894 50uM None
SA054213KS0904 50uM None
SA054214KS0914 50uM None
SA054215KS0924 50uM None
SA054216KS0934 None 100ng/ml
SA054217KS0944 None 100ng/ml
SA054218KS0954 None 100ng/ml
SA054219KS0964 None 100ng/ml
SA054208KS0854 None None
SA054209KS0864 None None
SA054210KS0874 None None
SA054211KS0884 None None
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Collection:

Collection ID:CO000005
Collection Summary:-
Sample Type:Macrophages

Treatment:

Treatment ID:TR000007

Sample Preparation:

Sampleprep ID:SP000010
Sampleprep Summary:-

Combined analysis:

Analysis ID AN000012
Analysis type MS
Chromatography type
Chromatography system Multiple
Column Multiple
MS Type Multiple
MS instrument type Multiple
MS instrument name Multiple
Ion Mode UNSPECIFIED
Units pmol/ug DNA

Chromatography:

Chromatography ID:CH000005
Instrument Name:Multiple
Column Name:Multiple

MS:

MS ID:MS000012
Analysis ID:AN000012
Instrument Name:Multiple
Instrument Type:Multiple
MS Type:Multiple
Ion Mode:UNSPECIFIED
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