Summary of Study ST000042

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000041. The data can be accessed directly via it's Project DOI: 10.21228/M8SG64 This work is supported by NIH grant, U2C- DK119886.

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Study IDST000042
Study TitleBALF Control vs ALI by RPLC-MS
Study TypeMS
Study SummaryAcute respiratory distress syndrome (ARDS) remains a significant hazard to human health and is clinically challenging because there are no prognostic biomarkers and no effective pharmacotherapy. The lung compartment metabolome may detail the status of the local environment that could be useful in ARDS biomarker discovery and the identification of drug target opportunities. However, neither the utility of bronchoalveolar lavage fluid (BALF) as a biofluid for metabolomics nor the optimal analytical platform for metabolite identification are established. To address this, we undertook a study to compare metabolites in BALF samples from patients with ARDS and healthy controls using a newly developed liquid chromatography (LC)-mass spectroscopy (MS) platform for untargeted metabolomics. Following initial testing of three different high performance liquid chromatography (HPLC) columns, we determined that reversed phase (RP)-LC and hydrophilic interaction chromatography (HILIC), were the most informative chromatographic methods because they yielded the most and highest quality data. Following confirmation of metabolite identification, statistical analysis resulted in 37 differentiating metabolites in the BALF of ARDS compared with health across both analytical platforms. Pathway analysis revealed networks associated with amino acid metabolism, glycolysis and gluconeogenesis, fatty acid biosynthesis, phospholipids and purine metabolism in the ARDS BALF. The complementary analytical platforms of RPLC and HILIC-LC generated informative, insightful metabolomics data of the ARDS lung environment. Research is published: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4068805/
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Email mkachman@umich.edu
Submit Date2014-03-22
Num Groups2
Total Subjects23
Raw Data AvailableYes
Raw Data File Type(s)d
Uploaded File Size7.7 G
Analysis Type DetailLC-MS
Release Date2014-04-21
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8SG64
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000041
Project DOI:doi: 10.21228/M8SG64
Project Title:BALF Studies
Project Type:MS study
Project Summary:Studies of BALF as a biofluid for metabolomics
Institute:University of Michigan
Department:UMich College of Pharmacy
Laboratory:Stringer Lab
Last Name:Stringer
First Name:Kathleen
Address:Ann Arbor, MI
Email:stringek@umich.edu
Phone:734-647-4775

Subject:

Subject ID:SU000059
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Human

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Condition
SA001811S00010329Acute lung injury
SA001812S00010328Acute lung injury
SA001813S00010327Acute lung injury
SA001814S00010326Acute lung injury
SA001815S00010330Acute lung injury
SA001816S00010331Acute lung injury
SA001817S00010335Acute lung injury
SA001818S00010334Acute lung injury
SA001819S00010333Acute lung injury
SA001820S00010332Acute lung injury
SA001821S00010325Acute lung injury
SA001822S00010324Acute lung injury
SA001823S00010313Acute lung injury
SA001824S00010318Acute lung injury
SA001825S00010317Acute lung injury
SA001826S00010315Acute lung injury
SA001827S00010314Acute lung injury
SA001828S00010323Acute lung injury
SA001829S00010322Acute lung injury
SA001830S00010316Acute lung injury
SA001831S00010319Healthy
SA001832S00010321Healthy
SA001833S00010320Healthy
Showing results 1 to 23 of 23

Collection:

Collection ID:CO000042
Collection Summary:-
Sample Type:Bronchoalveolar Lavage Fluid

Treatment:

Treatment ID:TR000060

Sample Preparation:

Sampleprep ID:SP000055
Sampleprep Summary:-
Sampleprep Protocol Filename:EX00184-Sample_preparation_protocol.pdf

Combined analysis:

Analysis ID AN000064 AN000065
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Agilent 1200 Agilent 1200
Column Waters Acquity HSS T3 Waters Acquity HSS T3
MS Type ESI ESI
MS instrument type QTOF QTOF
MS instrument name Agilent 6530 QTOF Agilent 6530 QTOF
Ion Mode POSITIVE NEGATIVE
Units Counts Counts

Chromatography:

Chromatography ID:CH000043
Methods ID:AQM003:AQM004
Methods Filename:Column1_lowinj_jetstream_grad9.m.zip
Instrument Name:Agilent 1200
Column Name:Waters Acquity HSS T3
Chromatography Type:Reversed phase

MS:

MS ID:MS000045
Analysis ID:AN000064
Instrument Name:Agilent 6530 QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:POSITIVE
Acquisition Parameters File:Column1_solv1_lowinj_jetstream_pos_grad9.m.zip
Processing Parameters File:LCPosDataProcessing.m
Analysis Protocol File:Metabolomic_analysis_of_lung_lavage_fluid_RP-LC-MS.docx
  
MS ID:MS000046
Analysis ID:AN000065
Instrument Name:Agilent 6530 QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:NEGATIVE
Acquisition Parameters File:Column1_solv2_lowinj_jetstream_neg_grad9.m.zip
Processing Parameters File:LCNegDataProcessing.m
Analysis Protocol File:Metabolomic_analysis_of_lung_lavage_fluid_RP-LC-MS.docx
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