Summary of Study ST000072

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000069. The data can be accessed directly via it's Project DOI: 10.21228/M8KS37 This work is supported by NIH grant, U2C- DK119886.

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Study IDST000072
Study TitleEffect of diet and age on ovarian metabolome (via tissue)
Study SummaryOvarian samples from twenty-one adult, female Cynomolgus monkeys were studied, eight of which were fed the Western #907 diet, and 13 of which were fed the Prudent #611 diet. HPLC-MS data were acquired for the 21 samples.
Institute
University of North Carolina
DepartmentDiscovery and Analytical Sciences (DAS)
LaboratorySumner Lab
Last NameSumner
First NameSusan
AddressEastern Regional Comprehensive Metabolomics Resource Core, UNC Nutrition Research Institute, 500 Laureate Way, Kannapolis, NC, 28081
Emailsusan_sumner @unc.edu
Phone704-250-5066
Submit Date2014-06-14
Num Groups2
Total Subjects21
Raw Data AvailableNo
Raw Data File Type(s)raw(Waters)
Analysis Type DetailLC-MS
Release Date2015-07-31
Release Version1
Susan Sumner Susan Sumner
https://dx.doi.org/10.21228/M8KS37
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000069
Project DOI:doi: 10.21228/M8KS37
Project Title:Effect of diet and age on ovarian metabolome
Project Type:Effects of Western diet on female reproductive function and aging, including effects associated with oxidative stress pathways
Project Summary:The long-term goal of this research is to understand the effects of the Western diet on female reproductive function and aging, to characterize the resultant health consequences and to determine the value of dietary intervention. In the past 100 years, dietary patterns in Western societies have changed remarkably. The consumption of saturated fatty acids (SFA), omega-6 (n-6) unsaturated fatty acids and refined carbohydrates has risen sharply, whereas consumption of fruits, vegetables and omega-3 fatty acids (n-3) has declined. This dietary pattern is associated with post-prandial oxidative stress and inflammation, both of which are implicated in numerous disease processes. The key rationale for this pilot grant application stems from studies reporting a decline in reproductive function in women that is coincident with changing patterns of Western diet, observations of Western diet-associated infertility in rodents, and adverse effects of diet on ovarian reserve in monkeys. Recently, ovarian-metabolomic profiling has been used to characterize oxidative and inflammatory pathways in the non-diseased ovary. Using this technology, it has also been reported that aging women, and those with reduced ovarian reserve, have altered follicular fluid levels of carbohydrates and reproductive hormones compared to normal ovarian reserve women. Therefore, this methodology is ideal for determining differences in global metabolomic profiles among ovaries derived from subjects of differing nutritional backgrounds. The proposed study will compare the effects of chronic exposure to a typical Western diet with a prudent diet on ovarian metabolome and metabolites associated with oxidative stress pathways. The central hypothesis is that ovaries from monkeys exposed to markedly divergent nutritional backgrounds will differentiate from each other with respect to their metabolome and with respect to metabolites indicative of oxidative stress. We will address this with three Specific Aims designed to: 1) determine whether the metabolome of ovarian tissue from cynomolgus monkeys (Macaca fascicularis) differentiates by nutritional background; 2) determine if metabolomic profiles of ovarian tissue will differentiate by age in pedigreed, known-age domestic vervet monkeys (Chlorocebus aethiops sabaeus) and 3) investigate whether the effects of diet or age on ovarian tissue metabolome is reflected by changes in serum metabolites. Archived ovarian tissue from two well-established nonhuman primate models of women’s health (Macaca fascicularis and Chlorocebus aethiops sabaeus) will be used for this study.
Institute:Wake Forest University
Department:Department of Pathology
Last Name:Appt
First Name:Susan
Address:Medical Center Blvd., Winston-Salem, NC 27157
Email:sappt@wakehealth.edu
Phone:336-716-1637

Subject:

Subject ID:SU000091
Subject Type:Animal
Subject Species:Macaca fascicularis
Taxonomy ID:9541
Age Or Age Range:7.5-18.17
Gender:Female
Animal Housing:pen
Animal Feed:Prudent #611 or Wester #907
Species Group:Mammal

Factors:

Subject type: Animal; Subject species: Macaca fascicularis (Factor headings shown in green)

mb_sample_id local_sample_id Diet
SA003735Pooled All_1Pooled All
SA003736Pooled All_2Pooled All
SA003737Pooled All_3Pooled All
SA003738Pooled Prudent_3Pooled Prudent_611
SA003739Pooled Prudent_2Pooled Prudent_611
SA003740Pooled Prudent_1Pooled Prudent_611
SA003755Pooled Western_2Pooled Western_907
SA003756Pooled Western_1Pooled Western_907
SA003757Pooled Western_3Pooled Western_907
SA0037416644Prudent_611
SA0037426643Prudent_611
SA0037436634Prudent_611
SA0037446641Prudent_611
SA0037456653Prudent_611
SA0037466652Prudent_611
SA0037476655Prudent_611
SA0037486638Prudent_611
SA0037496651Prudent_611
SA0037506659Prudent_611
SA0037516704Prudent_611
SA0037526703Prudent_611
SA0037536648Prudent_611
SA0037547236Western_907
SA0037587279Western_907
SA0037597295Western_907
SA0037607284Western_907
SA0037617287Western_907
SA0037627275Western_907
SA0037637017Western_907
SA0037647018Western_907
Showing results 1 to 30 of 30

Collection:

Collection ID:CO000074
Collection Summary:-
Collection Protocol Comments:Snap frozen in liquid nitrogen
Sample Type:Ovary tissue
Volumeoramount Collected:128-597mg
Storage Conditions:-80C

Treatment:

Treatment ID:TR000092

Sample Preparation:

Sampleprep ID:SP000087
Sampleprep Summary:Ovary samples were extracted using acetonitrile protein precipitation. Samples were then dried and reconstituted for LC-MS analysis in 95:5 water:methanol.
Sampleprep Protocol Filename:RTI_APPT-OVARY_Metabolomics_Procedure.docx
Processing Method:Homogenization
Extraction Method:Acetonitrile
Extract Storage:-80 C
Sample Resuspension:95:5 Water:Methanol
Sample Spiking:L-Tryptophan-d5
Organ:Ovary

Combined analysis:

Analysis ID AN000113 AN000114
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Waters Acquity Waters Acquity
Column Waters Acquity HSS T3 (100 x 2.1mm,1.8um) Waters Acquity HSS T3 (100 x 2.1mm,1.8um)
MS Type ESI ESI
MS instrument type QTOF QTOF
MS instrument name Waters Synapt G2 QTOF Waters Synapt G2 QTOF
Ion Mode POSITIVE NEGATIVE
Units Normalized abundance Normalized abundance

Chromatography:

Chromatography ID:CH000078
Instrument Name:Waters Acquity
Column Name:Waters Acquity HSS T3 (100 x 2.1mm,1.8um)
Column Pressure:6000-10000
Column Temperature:50 C
Flow Gradient:Time(min) Flow Rate %A %B Curve ; 1. Initial 0.400 100.0 0.0 ; 2. 1.00 0.400 100.0 0.0 6 ; 3. 16.00 0.400 0.0 100.0 6 ; 4. 20.00 0.400 0.0 100.0 6 ; 5. 22.00 0.400 100.0 0.0 6
Flow Rate:0.4 mL/min
Injection Temperature:8 C
Internal Standard:L-Tryptophan-d5
Solvent A:100% water; 0.1% formic acid
Solvent B:100% methanol; 0.1% formic acid
Analytical Time:22 min
Weak Wash Solvent Name:5%MeOH
Weak Wash Volume:1000 uL
Strong Wash Solvent Name:80%MeOH
Strong Wash Volume:1000 uL
Target Sample Temperature:8 C
Sample Loop Size:10 uL
Sample Syringe Size:100 uL
Randomization Order:Yes
Chromatography Type:Reversed phase

MS:

MS ID:MS000089
Analysis ID:AN000113
Instrument Name:Waters Synapt G2 QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:POSITIVE
Capillary Temperature:110 C
Capillary Voltage:3.2 kV
Collision Energy:4
Fragmentation Method:CID
Helium Flow:180
Ionization:ES+
Mass Accuracy:10 ppm
Source Temperature:110 C
Dataformat:Continuum
Desolvation Gas Flow:400 L/Hr
Desolvation Temperature:400 C
Resolution Setting:18000
Scan Range Moverz:50-1000 m/s
Scanning Cycle:1 s
Tube Lens Voltage:75
  
MS ID:MS000090
Analysis ID:AN000114
Instrument Name:Waters Synapt G2 QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:NEGATIVE
Capillary Temperature:110 C
Capillary Voltage:2.8 kV
Collision Energy:4
Fragmentation Method:CID
Helium Flow:180
Ionization:ES-
Source Temperature:110 C
Dataformat:Continuum
Desolvation Gas Flow:400 L/Hr
Desolvation Temperature:400 C
Resolution Setting:18000
Scan Range Moverz:50-1000 m/z
Scanning Cycle:1 s
Tube Lens Voltage:74
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