Summary of Study ST000073
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000069. The data can be accessed directly via it's Project DOI: 10.21228/M8KS37 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000073 |
| Study Title | Effect of diet and age on ovarian metabolome (serum metabolome compared to ovarian) |
| Study Summary | Serum samples from twenty-one adult, female Cynomolgus monkeys were studied, eight of which were fed the Western #907 diet, and 13 of which were fed the Prudent #611 diet. HPLC-MS data were acquired for the 21 samples. |
| Institute | University of North Carolina |
| Department | Discovery and Analytical Sciences (DAS) |
| Laboratory | Sumner Lab |
| Last Name | Sumner |
| First Name | Susan |
| Address | Eastern Regional Comprehensive Metabolomics Resource Core, UNC Nutrition Research Institute, 500 Laureate Way, Kannapolis, NC, 28081 |
| susan_sumner @unc.edu | |
| Phone | 704-250-5066 |
| Submit Date | 2014-06-14 |
| Num Groups | 2 |
| Total Subjects | 21 |
| Raw Data Available | No |
| Raw Data File Type(s) | raw(Waters) |
| Analysis Type Detail | LC-MS |
| Release Date | 2015-07-31 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000069 |
| Project DOI: | doi: 10.21228/M8KS37 |
| Project Title: | Effect of diet and age on ovarian metabolome |
| Project Type: | Effects of Western diet on female reproductive function and aging, including effects associated with oxidative stress pathways |
| Project Summary: | The long-term goal of this research is to understand the effects of the Western diet on female reproductive function and aging, to characterize the resultant health consequences and to determine the value of dietary intervention. In the past 100 years, dietary patterns in Western societies have changed remarkably. The consumption of saturated fatty acids (SFA), omega-6 (n-6) unsaturated fatty acids and refined carbohydrates has risen sharply, whereas consumption of fruits, vegetables and omega-3 fatty acids (n-3) has declined. This dietary pattern is associated with post-prandial oxidative stress and inflammation, both of which are implicated in numerous disease processes. The key rationale for this pilot grant application stems from studies reporting a decline in reproductive function in women that is coincident with changing patterns of Western diet, observations of Western diet-associated infertility in rodents, and adverse effects of diet on ovarian reserve in monkeys. Recently, ovarian-metabolomic profiling has been used to characterize oxidative and inflammatory pathways in the non-diseased ovary. Using this technology, it has also been reported that aging women, and those with reduced ovarian reserve, have altered follicular fluid levels of carbohydrates and reproductive hormones compared to normal ovarian reserve women. Therefore, this methodology is ideal for determining differences in global metabolomic profiles among ovaries derived from subjects of differing nutritional backgrounds. The proposed study will compare the effects of chronic exposure to a typical Western diet with a prudent diet on ovarian metabolome and metabolites associated with oxidative stress pathways. The central hypothesis is that ovaries from monkeys exposed to markedly divergent nutritional backgrounds will differentiate from each other with respect to their metabolome and with respect to metabolites indicative of oxidative stress. We will address this with three Specific Aims designed to: 1) determine whether the metabolome of ovarian tissue from cynomolgus monkeys (Macaca fascicularis) differentiates by nutritional background; 2) determine if metabolomic profiles of ovarian tissue will differentiate by age in pedigreed, known-age domestic vervet monkeys (Chlorocebus aethiops sabaeus) and 3) investigate whether the effects of diet or age on ovarian tissue metabolome is reflected by changes in serum metabolites. Archived ovarian tissue from two well-established nonhuman primate models of womens health (Macaca fascicularis and Chlorocebus aethiops sabaeus) will be used for this study. |
| Institute: | Wake Forest University |
| Department: | Department of Pathology |
| Last Name: | Appt |
| First Name: | Susan |
| Address: | Medical Center Blvd., Winston-Salem, NC 27157 |
| Email: | sappt@wakehealth.edu |
| Phone: | 336-716-1637 |
Subject:
| Subject ID: | SU000092 |
| Subject Type: | Animal |
| Subject Species: | Macaca fascicularis |
| Taxonomy ID: | 9541 |
| Age Or Age Range: | 7.5-18.17 |
| Gender: | Female |
| Animal Housing: | pen |
| Animal Feed: | Prudent #611 or Wester #907 |
| Species Group: | Mammals |
Factors:
Subject type: Animal; Subject species: Macaca fascicularis (Factor headings shown in green)
| mb_sample_id | local_sample_id | Diet | Age |
|---|---|---|---|
| SA003765 | Pooled All_1 | -- | -- |
| SA003766 | Pooled All_2 | -- | -- |
| SA003767 | Pooled All_3 | -- | -- |
| SA003768 | Pooled Prudent_3 | Prudent_#611 | -- |
| SA003769 | Pooled Prudent_2 | Prudent_#611 | -- |
| SA003770 | Pooled Prudent_1 | Prudent_#611 | -- |
| SA003771 | 6644 | Prudent_#611 | 13.17 |
| SA003772 | 6643 | Prudent_#611 | 13.17 |
| SA003773 | 6634 | Prudent_#611 | 13.17 |
| SA003774 | 6641 | Prudent_#611 | 13.17 |
| SA003775 | 6653 | Prudent_#611 | 13.17 |
| SA003776 | 6652 | Prudent_#611 | 14.17 |
| SA003777 | 6655 | Prudent_#611 | 14.17 |
| SA003778 | 6638 | Prudent_#611 | 14.17 |
| SA003779 | 6651 | Prudent_#611 | 15.17 |
| SA003780 | 6659 | Prudent_#611 | 18.17 |
| SA003781 | 6704 | Prudent_#611 | 7.5 |
| SA003782 | 6703 | Prudent_#611 | 7.5 |
| SA003783 | 6648 | Prudent_#611 | 9.17 |
| SA003784 | 7236 | Western_#0987 | 10.2 |
| SA003785 | Pooled Western_2 | Western_#907 | -- |
| SA003786 | Pooled Western_1 | Western_#907 | -- |
| SA003787 | Pooled Western_3 | Western_#907 | -- |
| SA003788 | 7279 | Western_#907 | 10.09 |
| SA003789 | 7295 | Western_#907 | 10.21 |
| SA003790 | 7284 | Western_#907 | 13.07 |
| SA003791 | 7287 | Western_#907 | 14.09 |
| SA003792 | 7275 | Western_#907 | 17.06 |
| SA003793 | 7017 | Western_#907 | 17.84 |
| SA003794 | 7018 | Western_#907 | 7.82 |
| Showing results 1 to 30 of 30 |
Collection:
| Collection ID: | CO000075 |
| Collection Summary: | - |
| Collection Protocol Comments: | Snap frozen in liquid nitrogen |
| Sample Type: | Ovary tissue |
| Volumeoramount Collected: | 128-597mg |
| Storage Conditions: | -80C |
Treatment:
| Treatment ID: | TR000093 |
Sample Preparation:
| Sampleprep ID: | SP000088 |
| Sampleprep Summary: | Ovary samples were extracted using acetonitrile protein precipitation. Samples were then dried and reconstituted for LC-MS analysis in 95:5 water:methanol. |
| Sampleprep Protocol Filename: | RTI_APPT-SERUM_Metabolomics_Procedure.docx |
| Processing Method: | Homogenization |
| Extraction Method: | Acetonitrile |
| Extract Storage: | -80 C |
| Sample Resuspension: | 95:5 Water:Methanol |
| Sample Spiking: | L-Tryptophan-d5 |
| Organ: | Ovary |
Combined analysis:
| Analysis ID | AN000115 | AN000116 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Waters Acquity | Waters Acquity |
| Column | Waters Acquity HSS T3 (100 x 2.1mm,1.8um) | Waters Acquity HSS T3 (100 x 2.1mm,1.8um) |
| MS Type | ESI | ESI |
| MS instrument type | QTOF | QTOF |
| MS instrument name | Waters Synapt G2 | Waters Synapt G2 |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Normalized abundance | Normalized abundance |
Chromatography:
| Chromatography ID: | CH000079 |
| Instrument Name: | Waters Acquity |
| Column Name: | Waters Acquity HSS T3 (100 x 2.1mm,1.8um) |
| Column Pressure: | 6000-10000 |
| Column Temperature: | 50 C |
| Flow Gradient: | Time(min) Flow Rate %A %B Curve ; 1. Initial 0.400 100.0 0.0 ; 2. 1.00 0.400 100.0 0.0 6 ; 3. 16.00 0.400 0.0 100.0 6 ; 4. 20.00 0.400 0.0 100.0 6 ; 5. 22.00 0.400 100.0 0.0 6 |
| Flow Rate: | 0.4 mL/min |
| Injection Temperature: | 8 C |
| Internal Standard: | L-Tryptophan-d5 |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% methanol; 0.1% formic acid |
| Analytical Time: | 22 min |
| Weak Wash Solvent Name: | 5%MeOH |
| Weak Wash Volume: | 1000 uL |
| Strong Wash Solvent Name: | 80%MeOH |
| Strong Wash Volume: | 1000 uL |
| Target Sample Temperature: | 8 C |
| Sample Loop Size: | 10 uL |
| Sample Syringe Size: | 100 uL |
| Randomization Order: | Yes |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS000091 |
| Analysis ID: | AN000115 |
| Instrument Name: | Waters Synapt G2 |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| Capillary Temperature: | 110 C |
| Capillary Voltage: | 3.2 kV |
| Collision Energy: | 4 |
| Fragmentation Method: | CID |
| Helium Flow: | 180 |
| Ionization: | ES+ |
| Mass Accuracy: | 10 ppm |
| Source Temperature: | 110 C |
| Dataformat: | Continuum |
| Desolvation Gas Flow: | 400 L/Hr |
| Desolvation Temperature: | 400 C |
| Resolution Setting: | 18000 |
| Scan Range Moverz: | 50-1000 m/s |
| Scanning Cycle: | 1 s |
| Tube Lens Voltage: | 75 |
| MS ID: | MS000092 |
| Analysis ID: | AN000116 |
| Instrument Name: | Waters Synapt G2 |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
| Capillary Temperature: | 110 C |
| Capillary Voltage: | 2.8 kV |
| Collision Energy: | 4 |
| Fragmentation Method: | CID |
| Helium Flow: | 180 |
| Ionization: | ES- |
| Source Temperature: | 110 C |
| Dataformat: | Continuum |
| Desolvation Gas Flow: | 400 L/Hr |
| Desolvation Temperature: | 400 C |
| Resolution Setting: | 18000 |
| Scan Range Moverz: | 50-1000 m/z |
| Scanning Cycle: | 1 s |
| Tube Lens Voltage: | 74 |
