Summary of Study ST000076
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000073. The data can be accessed directly via it's Project DOI: 10.21228/M8B88S This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000076 |
| Study Title | A549 Cell Study |
| Study Type | tracer |
| Study Summary | 13C-Glu and 13C/15N-Gln SIRM study of A549 cells |
| Institute | University of Kentucky |
| Department | School of Medicine |
| Last Name | Yan |
| First Name | Jun |
| jun.yan@louisville.edu | |
| Submit Date | 2014-07-02 |
| Num Groups | 3 |
| Total Subjects | 6 |
| Raw Data Available | No |
| Analysis Type Detail | GC-MS/LC-MS |
| Release Date | 2014-11-01 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000073 |
| Project DOI: | doi: 10.21228/M8B88S |
| Project Title: | CESB Lung Cancer Project |
| Project Type: | MS/NMR |
| Project Summary: | Influence of Microenvironmental Nutrient Availability on Systems Biochemistry of Lung Cancer Cells and Immuomodulation. This cell-based project aims to gain a systematic understanding of lung cancer metabolism and dysregulations as a function of key microenvironmental factors that govern lung cancer cell development and its ability to evade immune surveillance. The factors to be examined include the availability of key nutrients crucial to cancer proliferation, survival, metastasis, and immunoregulation. |
| Institute: | University of Kentucky |
| Department: | Toxicology |
| Last Name: | Fan |
| First Name: | Teresa |
| Email: | twmfan@gmail.com |
Subject:
| Subject ID: | SU000095 |
| Subject Type: | Human cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Genotype Strain: | A549 |
| Species Group: | Human |
Factors:
Subject type: Human cells; Subject species: Homo sapiens (Factor headings shown in green)
| mb_sample_id | local_sample_id | Labeling |
|---|---|---|
| SA003963 | 13C/15N-Gln-plate-1-cells | 13C/15N-Gln |
| SA003964 | 13C/15N-Gln-plate-2-cells | 13C/15N-Gln |
| SA003961 | 13C-Glc-plate-2-cells | 13C-Glc |
| SA003962 | 13C-Glc-plate-1-cells | 13C-Glc |
| SA003965 | unlabeled-plate-2-cells | unlabeled |
| SA003966 | unlabeled-plate-1-cells | unlabeled |
| Showing results 1 to 6 of 6 |
Collection:
| Collection ID: | CO000078 |
| Collection Summary: | cell_quench_collection |
| Collection Protocol Filename: | 14Jun17_Fan_Quench_cell_tissue_SOP.pdf |
| Collection Protocol Comments: | Collection and quench of cells in tissue culture |
| Sample Type: | Lung |
Treatment:
| Treatment ID: | TR000096 |
| Treatment Summary: | 13C-Glc|13C/15N-Gln|unlabeled |
| Treatment Protocol Filename: | 14Jun17Fan_Cell_Tracer_Ex_SOP.pdf |
| Treatment Protocol Comments: | Cell culture in 13C-Glc-labeled media | 13C/15N-Gln-labeled media | unlabeled media for 24h |
Sample Preparation:
| Sampleprep ID: | SP000091 |
| Sampleprep Summary: | protein_extraction|polar_preparation|polar_extraction |
| Sampleprep Protocol Filename: | 14June20Fan_Prot_Quant_SOP.pdf 14Jun30Fan_Prep_Polar_SOP.pdf 14Jun30Fan_Extract_Polar_Lipid_Prot_SOP.pdf |
| Sampleprep Protocol Comments: | Protein extraction and quantification|Preparation of polar samples for GCMS and NMR|Polar extraction from cells. |
Combined analysis:
| Analysis ID | AN000121 | AN000122 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | GC | GC |
| Chromatography system | ||
| Column | ||
| MS Type | ESI | EI |
| MS instrument type | Single quadrupole | GC-ITQ |
| MS instrument name | Thermo LTQ-FT | Thermo ITQ |
| Ion Mode | NEGATIVE | POSITIVE |
| Units | normalized corrected Peak area | Peak area |
Chromatography:
| Chromatography ID: | CH000083 |
| Chromatography Type: | GC |
MS:
| MS ID: | MS000097 |
| Analysis ID: | AN000121 |
| Instrument Name: | Thermo LTQ-FT |
| Instrument Type: | Single quadrupole |
| MS Type: | ESI |
| MS Comments: | FTMS Analytical Experiment with detection of compounds with unique CHONPS molecular formula |
| Ion Mode: | NEGATIVE |
| MS ID: | MS000098 |
| Analysis ID: | AN000122 |
| Instrument Name: | Thermo ITQ |
| Instrument Type: | GC-ITQ |
| MS Type: | EI |
| MS Comments: | GCMS Analytical Experiment with detection of compounds by comparison to standards. Derivatization is done with MTBSTFA (N-methyl-N-[tert-butyldimethylsilyl]trifluoroacetamide) |
| Ion Mode: | POSITIVE |
