Summary of Study ST000099
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000089. The data can be accessed directly via it's Project DOI: 10.21228/M8988G This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000099 |
| Study Title | NMR analysis of DMD mouse serum |
| Study Summary | Measurements were performed on serum using 6 month old C57/B10 control (n=6) and mdx (n=8) mice. 1D proton and carbon spectra were collected on these samples |
| Institute | University of Florida |
| Department | Department of Biochemistry and Molecular Biology and Southeastern Center for Integrated Metabolomics (SECIM) |
| Laboratory | Arthur Edison |
| Last Name | Edison |
| First Name | Arthur |
| Address | R3-226 Academic Research Building, Department of Biochemistry & Molecular Biology, PO Box 100245, Gainesville, FL 32610-0245 |
| aedison@ufl.edu | |
| Submit Date | 2014-09-15 |
| Num Groups | 2 |
| Total Subjects | 14 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | fid |
| Uploaded File Size | 12 M |
| Analysis Type Detail | NMR |
| Release Date | 2015-11-04 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000089 |
| Project DOI: | doi: 10.21228/M8988G |
| Project Title: | 13C Metabolomics: Applications at Natural Abundance |
| Institute: | University of Florida |
| Department: | Department of Biochemistry and Molecular Biology |
| Laboratory: | Edison |
| Last Name: | Edison |
| First Name: | Arthur |
| Address: | R3-226 Academic Research Building, Department of Biochemistry & Molecular Biology, PO Box 100245, Gainesville, FL 32610-0245 |
| Email: | aedison@ufl.edu |
| Phone: | 352-392-4535 |
Subject:
| Subject ID: | SU000118 |
| Subject Type: | Animal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57/BL/10 |
| Age Or Age Range: | 6 month old |
| Gender: | males |
| Species Group: | Mammal |
Factors:
Subject type: Animal; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Strain | model |
|---|---|---|---|
| SA005501 | 295303_m3 | C57/BL/10ScSn-Dmdmdx/J | mdx |
| SA005502 | 295302_m1 | C57/BL/10ScSn-Dmdmdx/J | mdx |
| SA005503 | 295303_m5 | C57/BL/10ScSn-Dmdmdx/J | mdx |
| SA005504 | 295303_m2 | C57/BL/10ScSn-Dmdmdx/J | mdx |
| SA005505 | 295303_m4 | C57/BL/10ScSn-Dmdmdx/J | mdx |
| SA005506 | 295302_m4 | C57/BL/10ScSn-Dmdmdx/J | mdx |
| SA005507 | 295302_m2 | C57/BL/10ScSn-Dmdmdx/J | mdx |
| SA005508 | 295302_m3 | C57/BL/10ScSn-Dmdmdx/J | mdx |
| SA005509 | 298005_m3 | C57/BL/10SnJ | Control |
| SA005510 | 298005_m4 | C57/BL/10SnJ | Control |
| SA005511 | 298005_m2 | C57/BL/10SnJ | Control |
| SA005512 | 298004_m2 | C57/BL/10SnJ | Control |
| SA005513 | 298004_m1 | C57/BL/10SnJ | Control |
| SA005514 | 298004_m3 | C57/BL/10SnJ | Control |
| Showing results 1 to 14 of 14 |
Collection:
| Collection ID: | CO000101 |
| Collection Summary: | Mouse serum measurements were performed on 6 replicates of 6 month old C57/B10 control and 8 replicates of mdx mice. Two hundred ?L of blood was collected by submandibular puncture, clotted and centrifuged to extract serum. The mice were then humanely euthanized under 2% isofluorene anesthesia. Serum was flash frozen in liquid nitrogen and stored at -80 °C. |
| Sample Type: | BLOOD |
| Collection Method: | submandibular puncture, |
| Collection Location: | University of Florida |
| Collection Frequency: | 1 |
| Volumeoramount Collected: | 200 microliters |
| Storage Conditions: | -80 degree celsius |
| Blood Serum Or Plasma: | BLOOD Serum |
Treatment:
| Treatment ID: | TR000119 |
| Animal Endp Euthanasia: | 2% Isofluorene |
Sample Preparation:
| Sampleprep ID: | SP000114 |
| Sampleprep Summary: | Approximately 100 ?L of serum was lyophilized and re-suspended in 40 ?L 99% D2O and transferred into 1.5 mm NMR tubes (Norell). |
| Processing Method: | lyophilized |
| Processing Storage Conditions: | -80 degree celsius |
| Extraction Method: | submandibular puncture |
Analysis:
| Analysis ID: | AN000163 |
| Laboratory Name: | Edison |
| Analysis Type: | NMR |
| Analysis Comments: | 1D 13C |
| Software Version: | VNMRJ |
| Operator Name: | Brittany Lee McMullen |
| Data Format: | Varian |
| Chromatography ID: | CH000113 |
| Num Factors: | 2 |
NMR:
| NMR ID: | NM000033 |
| Analysis ID: | AN000163 |
| Instrument Name: | Varian |
| Instrument Type: | FT-NMR |
| NMR Experiment Type: | 1D 1H |
| Spectrometer Frequency: | 600 MHz |
| NMR Probe: | 1.5 mm HTS cryoprobe |
| NMR Solvent: | D2O |
| NMR Tube Size: | 1.5 mm |
| Shimming Method: | Manual |
| Pulse Sequence: | 1D 1H CPMG |
| Water Suppression: | Presaturation |
| Pulse Width: | 45 |
| Receiver Gain: | 28 |
| Offset Frequency: | 4.77 |
| Chemical Shift Ref Cpd: | TSP |
| Temperature: | 22 degree Celsius |
| Number Of Scans: | 256 |
| Dummy Scans: | 8 |
| Acquisition Time: | 2.0s |
| Relaxation Delay: | 2s |
| Spectral Width: | 16 ppm |
| Num Data Points Acquired: | 38422 |
| Real Data Points: | 19211 |
| Zero Filling: | 2x |
| Apodization: | Cosine squared |
| Chemical Shift Ref Std: | TSP |
