Summary of Study ST000111
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000103. The data can be accessed directly via it's Project DOI: 10.21228/M84K5W This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000111 |
| Study Title | Study of biological variation in PC9 cell culture |
| Study Summary | Use extra plates seeded for workshop to investigate biological variation with no tracer and no treatment |
| Institute | University of Kentucky |
| Department | Toxicology |
| Last Name | Fan |
| First Name | Teresa |
| twmfan@gmail.com | |
| Submit Date | 2014-09-29 |
| Raw Data Available | No |
| Analysis Type Detail | GC-MS |
| Release Date | 2015-02-03 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000103 |
| Project DOI: | doi: 10.21228/M84K5W |
| Project Title: | RCSIRM Outreach and Education 2014 Metabolomics Workshop |
| Project Summary: | Demonstrating the utility and practicality of Stable Isotope Enriched Cell Culture to investigation of cell metabolism |
| Institute: | University of Kentucky |
| Department: | Toxicology |
| Last Name: | Fan |
| First Name: | Teresa |
| Email: | twmfan@gmail.com |
Subject:
| Subject ID: | SU000130 |
| Subject Type: | Human cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Genotype Strain: | PC9 |
| Species Group: | Mammals |
Factors:
Subject type: Human cells; Subject species: Homo sapiens (Factor headings shown in green)
| mb_sample_id | local_sample_id | timepoint |
|---|---|---|
| SA006060 | PC9_CTL_unL_27_29Jul14-plate | 24 hrs |
| SA006061 | PC9_CTL_unL_26_29Jul14-plate | 24 hrs |
| SA006062 | PC9_CTL_unL_28_29Jul14-plate | 24 hrs |
| SA006063 | PC9_CTL_unL_29_29Jul14-plate | 24 hrs |
| SA006064 | PC9_CTL_unL_30_29Jul14-plate | 24 hrs |
| SA006065 | PC9_CTL_unL_25_29Jul14-plate | 24 hrs |
| SA006066 | PC9_CTL_unL_24_29Jul14-plate | 24 hrs |
| SA006067 | PC9_CTL_unL_20_29Jul14-plate | 24 hrs |
| SA006068 | PC9_CTL_unL_21_29Jul14-plate | 24 hrs |
| SA006069 | PC9_CTL_unL_22_29Jul14-plate | 24 hrs |
| SA006070 | PC9_CTL_unL_23_29Jul14-plate | 24 hrs |
| SA006071 | PC9_CTL_unL_19_29Jul14-plate | 24 hrs |
| Showing results 1 to 12 of 12 |
Collection:
| Collection ID: | CO000114 |
| Collection Summary: | Plate media | Cell quench collection |
| Collection Protocol Filename: | 2014-07-29_WS_Fan_Quench_cell_tissue.pdf |
| Collection Protocol Comments: | media collection from plate | Collection and quench of cells in tissue culture |
| Sample Type: | Lung |
Treatment:
| Treatment ID: | TR000132 |
| Treatment Protocol Filename: | 2014-07-29_WS_Fan_Cell_Tracer_Ex.pdf |
| Treatment Protocol Comments: | cell culture in unlabeled media for 24h |
Sample Preparation:
| Sampleprep ID: | SP000127 |
| Sampleprep Summary: | Protein extraction | Polar extraction |
| Sampleprep Protocol Filename: | 2014-07-29_WS_Fan_Prep_Polar.pdf 2014-07-29_WS_Fan_Prot_Quant.pdf |
| Sampleprep Protocol Comments: | Preparation of polar samples for GCMS and NMR. | Protein extraction and quantification. |
Chromatography:
| Chromatography ID: | CH000125 |
| Chromatography Type: | GC |
Analysis:
| Analysis ID: | AN000186 |
| Laboratory Name: | RCSIRM |
| Analysis Type: | MS |
| Analysis Comments: | GCMS Analytical Experiment with detection of compounds by comparison to standards. Derivitization is done with MTBSTFA (N-methyl-N-[tert-butyldimethylsilyl]trifluoroacetamide). |
| Instrument Name: | Thermo ITQ GC-EI-MS |
| Chromatography ID: | CH000125 |
| Num Factors: | 1 |
| Num Metabolites: | 240 |
| Units: | Peak area |
