Summary of Study ST000111

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000103. The data can be accessed directly via it's Project DOI: 10.21228/M84K5W This work is supported by NIH grant, U2C- DK119886.

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Study IDST000111
Study TitleStudy of biological variation in PC9 cell culture
Study SummaryUse extra plates seeded for workshop to investigate biological variation with no tracer and no treatment
Institute
University of Kentucky
DepartmentToxicology
Last NameFan
First NameTeresa
Emailtwmfan@gmail.com
Submit Date2014-09-29
Raw Data AvailableNo
Analysis Type DetailGC-MS
Release Date2015-02-03
Release Version1
Teresa Fan Teresa Fan
https://dx.doi.org/10.21228/M84K5W
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000103
Project DOI:doi: 10.21228/M84K5W
Project Title:RCSIRM Outreach and Education 2014 Metabolomics Workshop
Project Summary:Demonstrating the utility and practicality of Stable Isotope Enriched Cell Culture to investigation of cell metabolism
Institute:University of Kentucky
Department:Toxicology
Last Name:Fan
First Name:Teresa
Email:twmfan@gmail.com

Subject:

Subject ID:SU000130
Subject Type:Human cells
Subject Species:Homo sapiens
Taxonomy ID:9606
Genotype Strain:PC9
Species Group:Mammals

Factors:

Subject type: Human cells; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id timepoint
SA006060PC9_CTL_unL_27_29Jul14-plate24 hrs
SA006061PC9_CTL_unL_26_29Jul14-plate24 hrs
SA006062PC9_CTL_unL_28_29Jul14-plate24 hrs
SA006063PC9_CTL_unL_29_29Jul14-plate24 hrs
SA006064PC9_CTL_unL_30_29Jul14-plate24 hrs
SA006065PC9_CTL_unL_25_29Jul14-plate24 hrs
SA006066PC9_CTL_unL_24_29Jul14-plate24 hrs
SA006067PC9_CTL_unL_20_29Jul14-plate24 hrs
SA006068PC9_CTL_unL_21_29Jul14-plate24 hrs
SA006069PC9_CTL_unL_22_29Jul14-plate24 hrs
SA006070PC9_CTL_unL_23_29Jul14-plate24 hrs
SA006071PC9_CTL_unL_19_29Jul14-plate24 hrs
Showing results 1 to 12 of 12

Collection:

Collection ID:CO000114
Collection Summary:Plate media | Cell quench collection
Collection Protocol Filename:2014-07-29_WS_Fan_Quench_cell_tissue.pdf
Collection Protocol Comments:media collection from plate | Collection and quench of cells in tissue culture
Sample Type:Lung

Treatment:

Treatment ID:TR000132
Treatment Protocol Filename:2014-07-29_WS_Fan_Cell_Tracer_Ex.pdf
Treatment Protocol Comments:cell culture in unlabeled media for 24h

Sample Preparation:

Sampleprep ID:SP000127
Sampleprep Summary:Protein extraction | Polar extraction
Sampleprep Protocol Filename:2014-07-29_WS_Fan_Prep_Polar.pdf
2014-07-29_WS_Fan_Prot_Quant.pdf
Sampleprep Protocol Comments:Preparation of polar samples for GCMS and NMR. | Protein extraction and quantification.

Combined analysis:

Analysis ID AN000186
Analysis type MS
Chromatography type GC
Chromatography system
Column
MS Type EI
MS instrument type GC-ITQ
MS instrument name Thermo ITQ
Ion Mode POSITIVE
Units Peak area

Chromatography:

Chromatography ID:CH000125
Chromatography Type:GC

MS:

MS ID:MS000149
Analysis ID:AN000186
Instrument Name:Thermo ITQ
Instrument Type:GC-ITQ
MS Type:EI
Ion Mode:POSITIVE
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