Summary of Study ST000122
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000109. The data can be accessed directly via it's Project DOI: 10.21228/M8C30B This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000122 |
| Study Title | Perinatal DDT causes dysfunctional lipid metabolism underlying metabolic syndrome |
| Study Type | steroid panel |
| Institute | University of California, Davis |
| Department | Nutrition |
| Laboratory | Gaikwad Lab |
| Last Name | Gaikwad |
| First Name | Nilesh |
| nwgaikwad@ucdavis.edu | |
| Submit Date | 2014-09-30 |
| Num Groups | NA |
| Raw Data Available | No |
| Analysis Type Detail | LC-MS |
| Release Date | 2014-09-30 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000109 |
| Project DOI: | doi: 10.21228/M8C30B |
| Project Title: | Perinatal DDT causes dysfunctional lipid metabolism underlying metabolic syndrome |
| Project Type: | Pilot and Feasibility Projects |
| Institute: | University of California, Davis |
| Department: | Nutrition |
| Laboratory: | Gaikwad Lab |
| Last Name: | Gaikwad |
| First Name: | Nilesh |
| Email: | nwgaikwad@ucdavis.edu |
| Phone: | 530-752-2906 |
| Funding Source: | NIH 1U24DK097154 ; PI Fiehn, Oliver ; UC Davis WEST COAST CENTRAL COMPREHENSIVE METABOLOMICS RESOURCE CORE (WC3MRC) |
Subject:
| Subject ID: | SU000141 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Human |
Factors:
Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)
| mb_sample_id | local_sample_id | Tissue/Fluid |
|---|---|---|
| SA006749 | CER346_246320_ML_28 | Serum |
| SA006750 | CER356_269662_ML_29 | Serum |
| SA006751 | CER368_250104_ML_30 | Serum |
| SA006752 | CER369_276355_ML_31 | Serum |
| SA006753 | CER340_244448_ML_27 | Serum |
| SA006754 | CER324_285069_ML_26 | Serum |
| SA006755 | CER287_248530_ML_23 | Serum |
| SA006756 | CER303_253023_ML_24 | Serum |
| SA006757 | CER315_282966_ML_25 | Serum |
| SA006758 | CER384_264971_ML_32 | Serum |
| SA006759 | CER445_286527_ML_33 | Serum |
| SA006760 | CER530_249229_ML_39 | Serum |
| SA006761 | CER540_240346_ML_40 | Serum |
| SA006762 | CER552_241945_ML_41 | Serum |
| SA006763 | CER555_251239_ML_42 | Serum |
| SA006764 | CER488_274343_ML_38 | Serum |
| SA006765 | CER483_294606_ML_37 | Serum |
| SA006766 | CER452_240972_ML_34 | Serum |
| SA006767 | CER463_271249_ML_35 | Serum |
| SA006768 | CER465_265004_ML_36 | Serum |
| SA006769 | CER277_255328_ML_22 | Serum |
| SA006770 | CER226_254303_ML_21 | Serum |
| SA006771 | CER110_238825_ML_7 | Serum |
| SA006772 | CER120_253690_ML_8 | Serum |
| SA006773 | CER147_254803_ML_9 | Serum |
| SA006774 | CER149_266689_ML_10 | Serum |
| SA006775 | CER093_242931_ML_6 | Serum |
| SA006776 | CER085_251176_ML_5 | Serum |
| SA006777 | CER040_242995_ML_2 | Serum |
| SA006778 | CER055_249947_ML_3 | Serum |
| SA006779 | CER062_246153_ML_4 | Serum |
| SA006780 | CER158_254231_ML_11 | Serum |
| SA006781 | CER165_287001_ML_12 | Serum |
| SA006782 | CER216_242490_ML_18 | Serum |
| SA006783 | CER220_274308_ML_19 | Serum |
| SA006784 | CER223_264067_ML_20 | Serum |
| SA006785 | CER201_244193_ML_17 | Serum |
| SA006786 | CER192_254091_ML_16 | Serum |
| SA006787 | CER178_295145_ML_13 | Serum |
| SA006788 | CER181_244392_ML_14 | Serum |
| SA006789 | CER188_250760_ML_15 | Serum |
| SA006790 | CER030_294717_ML_1 | Serum |
| Showing results 1 to 42 of 42 |
Collection:
| Collection ID: | CO000125 |
| Collection Summary: | - |
| Sample Type: | Blood |
Treatment:
| Treatment ID: | TR000143 |
Sample Preparation:
| Sampleprep ID: | SP000138 |
| Sampleprep Summary: | Methanol: Water Extraction |
| Sampleprep Protocol Filename: | NIH_WCMC_LaMerrill_Method_GaikwadLab__SteroidAnalysis_2013-14.docx |
| Processing Method: | Homogenization and Solvent Removal w/ Speed Vac |
| Processing Storage Conditions: | On Ice |
| Extraction Method: | 1:1 Methanol: Water |
| Extract Concentration Dilution: | 100mg or 1ml per 4ml 1:1 MeOH:H2O |
| Extract Storage: | -80C |
| Sample Resuspension: | 150ul CH3OH/H2O |
| Organ: | Sprague-Dawley Maternal: Adrenal, liver, placenta, amniotic fluid Sprague-Dawley Fetal: Male and female brain, male and female liver |
Combined analysis:
| Analysis ID | AN000204 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Waters Acquity |
| Column | Waters Acquity HSS T3 (150 x 2.1mm,1.8um) |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | Waters Xevo-TQ |
| Ion Mode | POSITIVE |
| Units | pg/ml |
Chromatography:
| Chromatography ID: | CH000136 |
| Chromatography Summary: | Targeted UPLC-MS/MS |
| Instrument Name: | Waters Acquity |
| Column Name: | Waters Acquity HSS T3 (150 x 2.1mm,1.8um) |
| Flow Gradient: | 0-2 min 100% A (Water 0.1% formic acid) 0% B (CH3CN 0.1 % formic acid), 2-4 min 80% A, 4-9mins 45% A, 9-11 mins 20% A, 11-12 mins 100% A |
| Flow Rate: | 0.15 ml/min |
| Sample Injection: | 10ul |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile; 0.1% formic acid |
| Analytical Time: | 12 mins |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS000167 |
| Analysis ID: | AN000204 |
| Instrument Name: | Waters Xevo-TQ |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| Capillary Voltage: | 3.0 kV |
| Collision Gas: | N2 |
| Ionization: | Electrospray Ionization |
| Source Temperature: | 150C |
| Desolvation Gas Flow: | 600 L/h |
| Desolvation Temperature: | 350C |
