Summary of Study ST000135
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000120. The data can be accessed directly via it's Project DOI: 10.21228/M82P4X This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000135 |
Study Title | LCMS analysis of Bile Acids |
Study Type | drug dosage |
Study Summary | Mice were dosed with TCDF for 5 days |
Institute | Pennsylvania State University |
Department | Molecular Toxicology |
Last Name | Patterson |
First Name | Andrew |
adp117@PSU.EDU, RGN5011@psu.edu | |
Submit Date | 2015-01-13 |
Num Groups | 5 |
Total Subjects | 58 |
Raw Data Available | No |
Raw Data File Type(s) | raw(Waters) |
Analysis Type Detail | LC-MS |
Release Date | 2015-01-13 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
Project ID: | PR000120 |
Project DOI: | doi: 10.21228/M82P4X |
Project Title: | Aryl Hydrocarbon Receptor Activation by Persistent Organic Pollutants Impacts Gut Microbiota-Host Metabolic Homeostasis in Mice |
Institute: | Pennsylvania State University |
Department: | Molecular Toxicology |
Last Name: | Patterson |
First Name: | Andrew |
Email: | adp117@PSU.EDU; RGN5011@psu.edu |
Subject:
Subject ID: | SU000154 |
Subject Type: | Animal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | C57BL/6J |
Age Or Age Range: | 6 weeks old |
Gender: | Males |
Species Group: | Mammals |
Factors:
Subject type: Animal; Subject species: Mus musculus (Factor headings shown in green)
mb_sample_id | local_sample_id | Gender | Genotype |
---|---|---|---|
SA007399 | 601 Ileum | Male | Tcdf Treated |
SA007400 | 404 Feces | Male | Tcdf Treated |
SA007401 | 402 Ileum | Male | Tcdf Treated |
SA007402 | 603 Feces | Male | Tcdf Treated |
SA007403 | 604 Ileum | Male | Tcdf Treated |
SA007404 | 601 Feces | Male | Tcdf Treated |
SA007405 | 402 Cecum | Male | Tcdf Treated |
SA007406 | 604 Cecum | Male | Tcdf Treated |
SA007407 | 402 Feces | Male | Tcdf Treated |
SA007408 | 601 Cecum | Male | Tcdf Treated |
SA007409 | 401 Feces | Male | Tcdf Treated |
SA007410 | 404 Cecum | Male | Tcdf Treated |
SA007411 | 603 Cecum | Male | Tcdf Treated |
SA007412 | 602 Cecum | Male | Tcdf Treated |
SA007413 | 603 Ileum | Male | Tcdf Treated |
SA007414 | 404 Ileum | Male | Tcdf Treated |
SA007415 | 601 Jejunum | Male | Tcdf Treated |
SA007416 | 404 Liver | Male | Tcdf Treated |
SA007417 | 602 Jejunum | Male | Tcdf Treated |
SA007418 | 603 Jejunum | Male | Tcdf Treated |
SA007419 | 604 Jejunum | Male | Tcdf Treated |
SA007420 | 603 Liver | Male | Tcdf Treated |
SA007421 | 601 Liver | Male | Tcdf Treated |
SA007422 | 602 Liver | Male | Tcdf Treated |
SA007423 | 602 Ileum | Male | Tcdf Treated |
SA007424 | 402 Jejunum | Male | Tcdf Treated |
SA007425 | 404 Jejunum | Male | Tcdf Treated |
SA007426 | 403 Feces | Male | Tcdf Treated |
SA007427 | 604 Liver | Male | Tcdf Treated |
SA007428 | 102 Feces | Male | Wild Type |
SA007429 | 302 Jejunum | Male | Wild Type |
SA007430 | 303 Jejunum | Male | Wild Type |
SA007431 | 304 Jejunum | Male | Wild Type |
SA007432 | 103 Feces | Male | Wild Type |
SA007433 | 301 Jejunum | Male | Wild Type |
SA007434 | 203 Jejunum | Male | Wild Type |
SA007435 | 202 Jejunum | Male | Wild Type |
SA007436 | 304 Cecum | Male | Wild Type |
SA007437 | 304 Ileum | Male | Wild Type |
SA007438 | 202 Ileum | Male | Wild Type |
SA007439 | 203 Ileum | Male | Wild Type |
SA007440 | 302 Ileum | Male | Wild Type |
SA007441 | 301 Ileum | Male | Wild Type |
SA007442 | 301 Liver | Male | Wild Type |
SA007443 | 303 Liver | Male | Wild Type |
SA007444 | 304 Liver | Male | Wild Type |
SA007445 | 302 Liver | Male | Wild Type |
SA007446 | 303 Ileum | Male | Wild Type |
SA007447 | 301 Cecum | Male | Wild Type |
SA007448 | 202 Feces | Male | Wild Type |
SA007449 | 203 Feces | Male | Wild Type |
SA007450 | 201 Feces | Male | Wild Type |
SA007451 | 202 Cecum | Male | Wild Type |
SA007452 | 203 Cecum | Male | Wild Type |
SA007453 | 302 Cecum | Male | Wild Type |
SA007454 | 303 Cecum | Male | Wild Type |
SA007455 | 201 Liver | Male | Wild Type |
SA007456 | 204 Feces | Male | Wild Type |
Showing results 1 to 58 of 58 |
Collection:
Collection ID: | CO000138 |
Collection Summary: | - |
Sample Type: | Feces, liver, small and large intestine |
Treatment:
Treatment ID: | TR000156 |
Treatment Summary: | Wild type and AHR knock out mice (5-6 per group) were treated with TCDF for 5 days |
Treatment: | Dietary |
Treatment Compound: | TCDF |
Treatment Route: | Oral |
Treatment Dose: | 24 µg/kg body weight |
Sample Preparation:
Sampleprep ID: | SP000151 |
Sampleprep Summary: | - |
Sampleprep Protocol Filename: | Bile_Acid_Extraction_for_LCMS.docx |
Processing Method: | Homogenization |
Processing Storage Conditions: | -20 |
Extraction Method: | Ethanol extraction |
Sample Spiking: | Used D4 standards of Cholic Acid , Lithocholic Acid, Ursodeoxycholic Acid, Chenodeoxycholic Acid, and Deoxycholic Acid |
Organ: | Liver, small and large intestine, and feces |
Combined analysis:
Analysis ID | AN000217 |
---|---|
Analysis type | MS |
Chromatography type | |
Chromatography system | |
Column | |
MS Type | ESI |
MS instrument type | Triple quadrupole |
MS instrument name | Waters |
Ion Mode | POSITIVE |
Units | Peak area |
Chromatography:
Chromatography ID: | CH000149 |
MS:
MS ID: | MS000169 |
Analysis ID: | AN000217 |
Instrument Name: | Waters |
Instrument Type: | Triple quadrupole |
MS Type: | ESI |
Ion Mode: | POSITIVE |