Summary of Study ST000136
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000121. The data can be accessed directly via it's Project DOI: 10.21228/M8XW20 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST000136 |
| Study Title | NIST lipidomics intralaboratory study |
| Study Type | Diet comparison |
| Study Summary | Targeted lipids were quantified and compared to the quantities of other labs across the nation. Comparisons were between pooled serum from humans who have consumed flax seed for the past month, pooled serums from humans who have consumed fish oil for the last month, and pooled serum from humans who have not consumed either flax seed or fish oil for the past month. Pooled samples were prepared in triplicate. |
| Institute | University of Florida |
| Department | College of Medicine, Department of Pathology, Immunology, and Laboratory Medicine |
| Laboratory | Biomedical Mass Spectometry Lab |
| Last Name | Patterson |
| First Name | Rainey |
| rpatterson@chem.ufl.edu | |
| Submit Date | 2014-01-16 |
| Num Groups | 4 |
| Total Subjects | 12 |
| Raw Data Available | No |
| Raw Data File Type(s) | mzXML |
| Analysis Type Detail | LC-MS |
| Release Date | 2016-06-18 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000121 |
| Project DOI: | doi: 10.21228/M8XW20 |
| Project Title: | NIST lipidomics intralaboratory study |
| Project Type: | Lipidomics, targeted |
| Project Summary: | Relative quantities of lipids were compared between three different human serum sample sets and compared to the results of other laboratories. Human serum samples were pooled from people who had either ingested fish oil supplements, flax seed, or neither for the last month. NIST SRM 1950 human pooled blood plasma was used for quality control and method development. Inidvidual lipids among several classes were targeted, including phosphoglycerolipids, triacylglycerides, diacylglycerides, sphingolipids, cholesterol and cholesteryl esters. |
| Institute: | University of Florida |
| Department: | College of Medicine, Department of pathology, immunology, and laboratory medicine |
| Laboratory: | Biomedical Mass Spectrometry Lab |
| Last Name: | Garrett |
| First Name: | Timothy |
| Email: | tgarrett@ufl.edu |
| Funding Source: | National Institutes of Health |
Subject:
| Subject ID: | SU000155 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Mammals |
Factors:
Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)
| mb_sample_id | local_sample_id | Diet |
|---|---|---|
| SA007460 | SRM2378 1_C | control |
| SA007461 | SRM2378 1_A | control |
| SA007462 | SRM2378 1_B | control |
| SA007463 | SRM2378 3_C | fish oil |
| SA007464 | SRM2378 3_A | fish oil |
| SA007465 | SRM2378 3_B | fish oil |
| SA007466 | SRM2378 2_C | flax seed |
| SA007467 | SRM2378 2_A | flax seed |
| SA007468 | SRM2378 2_B | flax seed |
| SA007457 | SRM 1950_A | QC |
| SA007458 | SRM 1950_C | QC |
| SA007459 | SRM 1950_B | QC |
| Showing results 1 to 12 of 12 |
Collection:
| Collection ID: | CO000139 |
| Collection Summary: | - |
| Collection Protocol ID: | SOP_LE_Matyash |
| Collection Protocol Filename: | SOP_LE_02_Matyash_RP_NIST.pdf |
| Sample Type: | blood |
| Storage Conditions: | -80C |
Treatment:
| Treatment ID: | TR000157 |
Sample Preparation:
| Sampleprep ID: | SP000152 |
| Sampleprep Summary: | MTBE lipid extraction |
| Sampleprep Protocol ID: | SOP_LE_Matyash |
| Sampleprep Protocol Filename: | SOP_LE_02_Matyash_RP_NIST.pdf |
| Processing Storage Conditions: | On ice |
| Extraction Method: | MTBE |
| Extract Concentration Dilution: | 40 uLplasma/40uL reconstitution solvent |
| Sample Resuspension: | 40 uL isopropanol |
| Sample Spiking: | Internal standards listed in protocol |
| Organ: | blodo plasma |
Combined analysis:
| Analysis ID | AN000218 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Thermo Dionex Ultimate 3000 |
| Column | Waters Acquity BEH C18 (50 x 2.1mm,1.7um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE |
| Units | nmol/mL serum |
Chromatography:
| Chromatography ID: | CH000150 |
| Chromatography Summary: | Reverse phase |
| Methods Filename: | m_lipids_AIF_pos |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | Waters Acquity BEH C18 (50 x 2.1mm,1.7um) |
| Column Pressure: | 800 bar (max) |
| Column Temperature: | 35 |
| Flow Gradient: | none |
| Flow Rate: | 0.5 mL/min |
| Injection Temperature: | 4 C |
| Sample Injection: | 3 uL for pos mode, 10 uL for neg mode |
| Solvent A: | 40% water/60% acetonitrile; 0.1% formic acid; 10 mM ammonium formate |
| Solvent B: | 90% isopropanol/8% acetonitrile/2% water; 0.1% formic acid; 10 mM ammonium formate |
| Analytical Time: | 20 min |
| Capillary Voltage: | 50 C |
| Running Voltage: | 3.5 kV |
| Randomization Order: | 3b,1b,1c,3c,2c,2a,2b,1a,3a |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS000170 |
| Analysis ID: | AN000218 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| Capillary Temperature: | 300 (+), 250 (-) |
| Collision Energy: | 25 NCE |
| Dry Gas Flow: | 30 (+), 25 (-) |
| Ion Spray Voltage: | 3.5 kV |
| Mass Accuracy: | < 2ppm |
| Precursor Type: | Dependent upon class |
| Spray Voltage: | 3.5 kV |
| Dataformat: | .RAW |
| Scan Range Moverz: | 120-2000 |
| Skimmer Voltage: | 15 |
| Acquisition Parameters File: | QE1_REP_34_sequence |
