Summary of Study ST000137

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000122. The data can be accessed directly via it's Project DOI: 10.21228/M8T59G This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

Study ID	ST000137
Study Title	Metabolomics in sarcoidosis
Study Type	observation
Study Summary	Metabolites in sarcoidosis patients
Institute	Wayne State University
Department	Internal Medicine
Last Name	Geamanu
First Name	Andreea
Email	ageamanu@med.wayne.edu
Submit Date	2015-02-11
Total Subjects	30
Raw Data Available	No
Analysis Type Detail	NMR
Release Date	2015-02-11
Release Version	1

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Project:

Project ID:	PR000122
Project DOI:	doi: 10.21228/M8T59G
Project Title:	Metabolomics in Sarcoidosis
Institute:	Wayne State University
Department:	Internal Medicine
Laboratory:	Samavati Lab
Last Name:	Samavati
First Name:	Lobelia
Email:	lsamavat@med.wayne.edu

Subject:

Subject ID:	SU000156
Subject Type:	Human
Subject Species:	Homo sapiens
Taxonomy ID:	9606
Species Group:	Human

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id	local_sample_id	Sample Group
SA007469	C49	Control
SA007470	C48	Control
SA007471	C51	Control
SA007472	C54	Control
SA007473	C28	Control
SA007474	C55	Control
SA007475	C46	Control
SA007476	C57	Control
SA007477	C31	Control
SA007478	C37	Control
SA007479	C33	Control
SA007480	C30	Control
SA007481	C36	Control
SA007482	C35	Control
SA007483	C34	Control
SA007484	P246	Patient
SA007485	P233	Patient
SA007486	P239	Patient
SA007487	P248	Patient
SA007488	P232	Patient
SA007489	P250	Patient
SA007490	P247	Patient
SA007491	P197	Patient
SA007492	P128	Patient
SA007493	P112	Patient
SA007494	P138	Patient
SA007495	P217	Patient
SA007496	P230	Patient
SA007497	P218	Patient
SA007498	P231	Patient

Showing results 1 to 30 of 30

Showing results 1 to 30 of 30

Collection:

Collection ID:	CO000140
Collection Summary:	-
Sample Type:	Serum

Treatment:

Treatment ID:	TR000158
Treatment Summary:	None

Sample Preparation:

Sampleprep ID:	SP000153
Sampleprep Summary:	Serum samples were analyzed following filtration through a 3-kD nominal cut-off membrane (Amicon Ultra 0.5 mL centrifugal filters, Millipore Corp, Billerica, MA) to remove proteins, lipids, lipoproteins, and protein-bound forms of certain molecules. Each serum sample was transferred to a prewashed filter and centrifuged at 13,800 g for 60 minutes as previously described (Gregory, Park et al. 2013). The filters were further washed with deuterium oxide (D2O) and the filtrate was mixed with the filtered serum in a 1:1 ratio. A reference buffer solution containing 5 mmol/L disodium-2,2-dimethyl 2-silapentane-5-sulphonate (DSS) and 10 mmol/L imidazole in D2O was added to the samples in a 1:9 ratio. The D2O provided a field-frequency lock, whereas DSS was used as the chemical shift reference and imidazole as pH indicator for the NMR spectra. A total of 600 µL mixture was transferred to a 5 mm NMR tube (Sigma Aldrich, St. Louis, MO).

Sampleprep ID:

SP000153

Sampleprep Summary:

Serum samples were analyzed following filtration through a 3-kD nominal cut-off membrane (Amicon Ultra 0.5 mL centrifugal filters, Millipore Corp, Billerica, MA) to remove proteins, lipids, lipoproteins, and protein-bound forms of certain molecules. Each serum sample was transferred to a prewashed filter and centrifuged at 13,800 g for 60 minutes as previously described (Gregory, Park et al. 2013). The filters were further washed with deuterium oxide (D2O) and the filtrate was mixed with the filtered serum in a 1:1 ratio. A reference buffer solution containing 5 mmol/L disodium-2,2-dimethyl 2-silapentane-5-sulphonate (DSS) and 10 mmol/L imidazole in D2O was added to the samples in a 1:9 ratio. The D2O provided a field-frequency lock, whereas DSS was used as the chemical shift reference and imidazole as pH indicator for the NMR spectra. A total of 600 µL mixture was transferred to a 5 mm NMR tube (Sigma Aldrich, St. Louis, MO).

Analysis:

Analysis ID:	AN000219
Analysis Type:	NMR
Chromatography ID:	CH000151
Num Factors:	2
Num Metabolites:	33

NMR:

NMR ID:	NM000051
Analysis ID:	AN000219
Instrument Name:	Agilent
Instrument Type:	FT-NMR
NMR Experiment Type:	NOESY
Spectrometer Frequency:	600 MHz