Summary of Study ST000145
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000129. The data can be accessed directly via it's Project DOI: 10.21228/M85C7W This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000145 |
| Study Title | Primary T Cell Noxa Knockdown (Donor 8) |
| Study Summary | Cells were deprived of glucose or glutamine for 1 h then resuspended in 10mM [13C1,2] D-glucose labeled or 4 mM [13C5]-U-glutamine labeled medium, respectively. At this time cells were also activated with 5 ug/ml anti-CD3 and anti-CD28. Cells were labeled (and activated) for 12 h when samples were spun down, and cells were resuspended in 200 ul ice cold methanol |
| Institute | University of Michigan |
| Department | Biomedical Research Core Facilities |
| Laboratory | Metabolomics core |
| Last Name | Kachman |
| First Name | Maureen |
| Address | 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 |
| mkachman@umich.edu | |
| Submit Date | 2015-03-13 |
| Num Groups | 3 |
| Total Subjects | 15 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Uploaded File Size | 3.8 G |
| Analysis Type Detail | GC-MS/LC-MS |
| Release Date | 2015-03-13 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000129 |
| Project DOI: | doi: 10.21228/M85C7W |
| Project Title: | Metabolic effects of Noxa |
| Project Summary: | labeled glutamine metabolomics on Noxa expressing cells |
| Institute: | University of Minnesota |
| Department: | Masonic Cancer Center |
| Laboratory: | Kelekar Lab |
| Last Name: | Kelekar |
| First Name: | Ameeta |
| Address: | 420 Delaware Street SE, Minneapolis, MN 55455 |
| Email: | ameeta@umn.edu |
| Phone: | 612-626-2358 |
Subject:
| Subject ID: | SU000164 |
| Subject Type: | Human cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Human |
Factors:
Subject type: Human cells; Subject species: Homo sapiens (Factor headings shown in green)
| mb_sample_id | local_sample_id | Label |
|---|---|---|
| SA008028 | S00016916 | Glucose |
| SA008029 | S00016929 | Glucose |
| SA008030 | S00016921 | Glucose |
| SA008031 | S00016920 | Glucose |
| SA008032 | S00016918 | Glucose |
| SA008033 | S00016919 | Glucose |
| SA008034 | S00016917 | Glucose |
| SA008035 | S00016928 | Glutamine |
| SA008036 | S00016930 | Glutamine |
| SA008037 | S00016927 | Glutamine |
| SA008038 | S00016923 | Glutamine |
| SA008039 | S00016922 | Glutamine |
| SA008040 | S00016924 | Glutamine |
| SA008041 | S00016925 | Glutamine |
| SA008042 | S00016926 | Glutamine |
| Showing results 1 to 15 of 15 |
Collection:
| Collection ID: | CO000150 |
| Collection Summary: | - |
| Sample Type: | Cells |
Treatment:
| Treatment ID: | TR000169 |
Sample Preparation:
| Sampleprep ID: | SP000164 |
| Sampleprep Summary: | - |
| Sampleprep Protocol Filename: | Fluxomics_analysis_protocol-2015-03-11.pdf |
Combined analysis:
| Analysis ID | AN000230 | AN000231 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | HILIC | GC |
| Chromatography system | Agilent 1260 | Agilent 7890A |
| Column | Phenomenex Luna NH2 (150 x 1mm,3um) | Agilent DB5-MS (30m × 0.25mm, 0.25um) |
| MS Type | ESI | EI |
| MS instrument type | QTOF | Single quadrupole |
| MS instrument name | Agilent 6520 QTOF | Agilent 5975C |
| Ion Mode | NEGATIVE | POSITIVE |
| Units | Area | Area |
Chromatography:
| Chromatography ID: | CH000160 |
| Methods ID: | AQM010|AQM011 |
| Methods Filename: | QTOF-002-HILIC-35min-1mm-No_Insert.m.zip ALPHA_KETO_ACIDS-FULL.M.zip |
| Instrument Name: | Agilent 1260 |
| Column Name: | Phenomenex Luna NH2 (150 x 1mm,3um) |
| Chromatography Type: | HILIC |
| Chromatography ID: | CH001300 |
| Methods ID: | AQM011 |
| Methods Filename: | ALPHA_KETO_ACIDS-FULL.M.zip |
| Instrument Name: | Agilent 7890A |
| Column Name: | Agilent DB5-MS (30m × 0.25mm, 0.25um) |
| Chromatography Type: | GC |
MS:
| MS ID: | MS000181 |
| Analysis ID: | AN000230 |
| Instrument Name: | Agilent 6520 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
| Acquisition Parameters File: | QTOF-002-HILIC-35min-1mm.m.zip |
| Processing Parameters File: | EX00383-MassHunterQuant-DataAnalysis-Method.m.zip |
| MS ID: | MS000182 |
| Analysis ID: | AN000231 |
| Instrument Name: | Agilent 5975C |
| Instrument Type: | Single quadrupole |
| MS Type: | EI |
| Ion Mode: | POSITIVE |
| Acquisition Parameters File: | ALPHA_KETO_ACIDS-FULL.M.zip |
