Summary of Study ST000145
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000129. The data can be accessed directly via it's Project DOI: 10.21228/M85C7W This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000145 |
Study Title | Primary T Cell Noxa Knockdown (Donor 8) |
Study Summary | Cells were deprived of glucose or glutamine for 1 h then resuspended in 10mM [13C1,2] D-glucose labeled or 4 mM [13C5]-U-glutamine labeled medium, respectively. At this time cells were also activated with 5 ug/ml anti-CD3 and anti-CD28. Cells were labeled (and activated) for 12 h when samples were spun down, and cells were resuspended in 200 ul ice cold methanol |
Institute | University of Michigan |
Department | Biomedical Research Core Facilities |
Laboratory | Metabolomics core |
Last Name | Kachman |
First Name | Maureen |
Address | 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 |
mkachman@umich.edu | |
Submit Date | 2015-03-13 |
Num Groups | 3 |
Total Subjects | 15 |
Raw Data Available | Yes |
Raw Data File Type(s) | d |
Uploaded File Size | 3.8 G |
Analysis Type Detail | GC-MS/LC-MS |
Release Date | 2015-03-13 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
Project ID: | PR000129 |
Project DOI: | doi: 10.21228/M85C7W |
Project Title: | Metabolic effects of Noxa |
Project Summary: | labeled glutamine metabolomics on Noxa expressing cells |
Institute: | University of Minnesota |
Department: | Masonic Cancer Center |
Laboratory: | Kelekar Lab |
Last Name: | Kelekar |
First Name: | Ameeta |
Address: | 420 Delaware Street SE, Minneapolis, MN 55455 |
Email: | ameeta@umn.edu |
Phone: | 612-626-2358 |
Subject:
Subject ID: | SU000164 |
Subject Type: | Human cells |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Species Group: | Mammals |
Factors:
Subject type: Human cells; Subject species: Homo sapiens (Factor headings shown in green)
mb_sample_id | local_sample_id | Label |
---|---|---|
SA008028 | S00016916 | Glucose |
SA008029 | S00016929 | Glucose |
SA008030 | S00016921 | Glucose |
SA008031 | S00016920 | Glucose |
SA008032 | S00016918 | Glucose |
SA008033 | S00016919 | Glucose |
SA008034 | S00016917 | Glucose |
SA008035 | S00016928 | Glutamine |
SA008036 | S00016930 | Glutamine |
SA008037 | S00016927 | Glutamine |
SA008038 | S00016923 | Glutamine |
SA008039 | S00016922 | Glutamine |
SA008040 | S00016924 | Glutamine |
SA008041 | S00016925 | Glutamine |
SA008042 | S00016926 | Glutamine |
Showing results 1 to 15 of 15 |
Collection:
Collection ID: | CO000150 |
Collection Summary: | - |
Sample Type: | Cells |
Treatment:
Treatment ID: | TR000169 |
Sample Preparation:
Sampleprep ID: | SP000164 |
Sampleprep Summary: | - |
Sampleprep Protocol Filename: | Fluxomics_analysis_protocol-2015-03-11.pdf |
Combined analysis:
Analysis ID | AN000230 | AN000231 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | HILIC | GC |
Chromatography system | Agilent 1260 | Agilent 7890A |
Column | Phenomenex Luna NH2 (150 x 1mm,3um) | Agilent DB5-MS (30m × 0.25mm, 0.25um) |
MS Type | ESI | EI |
MS instrument type | QTOF | Single quadrupole |
MS instrument name | Agilent 6520 QTOF | Agilent 5975C |
Ion Mode | NEGATIVE | POSITIVE |
Units | Area | Area |
Chromatography:
Chromatography ID: | CH000160 |
Methods ID: | AQM010|AQM011 |
Methods Filename: | QTOF-002-HILIC-35min-1mm-No_Insert.m.zip ALPHA_KETO_ACIDS-FULL.M.zip |
Instrument Name: | Agilent 1260 |
Column Name: | Phenomenex Luna NH2 (150 x 1mm,3um) |
Chromatography Type: | HILIC |
Chromatography ID: | CH001300 |
Methods ID: | AQM011 |
Methods Filename: | ALPHA_KETO_ACIDS-FULL.M.zip |
Instrument Name: | Agilent 7890A |
Column Name: | Agilent DB5-MS (30m × 0.25mm, 0.25um) |
Chromatography Type: | GC |
MS:
MS ID: | MS000181 |
Analysis ID: | AN000230 |
Instrument Name: | Agilent 6520 QTOF |
Instrument Type: | QTOF |
MS Type: | ESI |
Ion Mode: | NEGATIVE |
Acquisition Parameters File: | QTOF-002-HILIC-35min-1mm.m.zip |
Processing Parameters File: | EX00383-MassHunterQuant-DataAnalysis-Method.m.zip |
MS ID: | MS000182 |
Analysis ID: | AN000231 |
Instrument Name: | Agilent 5975C |
Instrument Type: | Single quadrupole |
MS Type: | EI |
Ion Mode: | POSITIVE |
Acquisition Parameters File: | ALPHA_KETO_ACIDS-FULL.M.zip |