Summary of Study ST000146
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000129. The data can be accessed directly via it's Project DOI: 10.21228/M85C7W This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000146 |
| Study Title | Noxa regulation of malate aspartate shuttle |
| Institute | University of Michigan |
| Department | Biomedical Research Core Facilities |
| Laboratory | Metabolomics core |
| Last Name | Kachman |
| First Name | Maureen |
| Address | 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 |
| mkachman@umich.edu | |
| Submit Date | 2015-03-13 |
| Num Groups | 6 |
| Total Subjects | 18 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Uploaded File Size | 1.4 G |
| Analysis Type Detail | LC-MS |
| Release Date | 2015-03-13 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000129 |
| Project DOI: | doi: 10.21228/M85C7W |
| Project Title: | Metabolic effects of Noxa |
| Project Summary: | labeled glutamine metabolomics on Noxa expressing cells |
| Institute: | University of Minnesota |
| Department: | Masonic Cancer Center |
| Laboratory: | Kelekar Lab |
| Last Name: | Kelekar |
| First Name: | Ameeta |
| Address: | 420 Delaware Street SE, Minneapolis, MN 55455 |
| Email: | ameeta@umn.edu |
| Phone: | 612-626-2358 |
Subject:
| Subject ID: | SU000165 |
| Subject Type: | Human cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Mammals |
Factors:
Subject type: Human cells; Subject species: Homo sapiens (Factor headings shown in green)
| mb_sample_id | local_sample_id | genotype |
|---|---|---|
| SA008043 | S00016946 | Noxa knockdown line |
| SA008044 | S00016945 | Noxa knockdown line |
| SA008045 | S00016947 | Noxa knockdown line |
| SA008046 | S00016955 | Noxa knockdown line medium |
| SA008047 | S00016956 | Noxa knockdown line medium |
| SA008048 | S00016954 | Noxa knockdown line medium |
| SA008049 | S00016942 | Noxa overexpressing line |
| SA008050 | S00016944 | Noxa overexpressing line |
| SA008051 | S00016943 | Noxa overexpressing line |
| SA008052 | S00016952 | Noxa overexpressing line medium |
| SA008053 | S00016953 | Noxa overexpressing line medium |
| SA008054 | S00016951 | Noxa overexpressing line medium |
| SA008055 | S00016939 | Parental line |
| SA008056 | S00016941 | Parental line |
| SA008057 | S00016940 | Parental line |
| SA008058 | S00016949 | Parental line medium |
| SA008059 | S00016948 | Parental line medium |
| SA008060 | S00016950 | Parental line medium |
| Showing results 1 to 18 of 18 |
Collection:
| Collection ID: | CO000151 |
| Collection Summary: | - |
| Sample Type: | Culture Media |
Treatment:
| Treatment ID: | TR000170 |
Sample Preparation:
| Sampleprep ID: | SP000165 |
| Sampleprep Summary: | - |
| Sampleprep Protocol Filename: | Fluxomics_analysis_protocol-2015-03-11.pdf |
Combined analysis:
| Analysis ID | AN000232 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Agilent 1260 |
| Column | Phenomenex Luna NH2 (150 x 1mm,3um) |
| MS Type | ESI |
| MS instrument type | QTOF |
| MS instrument name | Agilent 6520 QTOF |
| Ion Mode | NEGATIVE |
| Units | Area |
Chromatography:
| Chromatography ID: | CH000161 |
| Methods ID: | AQM010 |
| Methods Filename: | QTOF-002-HILIC-35min-1mm-No_Insert.m.zip |
| Instrument Name: | Agilent 1260 |
| Column Name: | Phenomenex Luna NH2 (150 x 1mm,3um) |
| Chromatography Type: | HILIC |
MS:
| MS ID: | MS000183 |
| Analysis ID: | AN000232 |
| Instrument Name: | Agilent 6520 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
| Acquisition Parameters File: | QTOF-002-HILIC-35min-1mm.m.zip |
| Processing Parameters File: | EX00384-MassHunterQuant-DataAnalysis-Method.m.zip |
