Summary of Study ST000147

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000129. The data can be accessed directly via it's Project DOI: 10.21228/M85C7W This work is supported by NIH grant, U2C- DK119886.

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Study IDST000147
Study Title13C targeted metabolomics
Study SummaryJurkat parental cells (JP6) or cells stably expressing the pCDNA Noxa vector (N5) cells were cultured to confluence and counted. 20E6 cells in triplicate were resuspended in glucose free or glutamine free medium for 2 hours. Cells were then pelleted and resuspended in 13C-1,2 Glucose (10mM) or 13C-U-glutamine (4mM) for 24 hours. Cells were pelleted and spent medium was collected. Cell pellets were washed 1X with ice cold PBS and cell pellets were resuspended in 400ul -20 methanol. Cells and media were snap frozen in liquid nitrogen and stored at -80.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Email mkachman@umich.edu
Submit Date2015-03-13
Num Groups8
Total Subjects24
Raw Data AvailableYes
Raw Data File Type(s)d
Uploaded File Size2.6 G
Analysis Type DetailGC-MS/LC-MS
Release Date2015-03-13
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M85C7W
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000129
Project DOI:doi: 10.21228/M85C7W
Project Title:Metabolic effects of Noxa
Project Summary:labeled glutamine metabolomics on Noxa expressing cells
Institute:University of Minnesota
Department:Masonic Cancer Center
Laboratory:Kelekar Lab
Last Name:Kelekar
First Name:Ameeta
Address:420 Delaware Street SE, Minneapolis, MN 55455
Email:ameeta@umn.edu
Phone:612-626-2358

Subject:

Subject ID:SU000166
Subject Type:Human cells
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Mammals

Factors:

Subject type: Human cells; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Treatment Cell type Harvest
SA008061S0001503313C glucose labeled Cloned Jurkat cell mid level Noxa cells
SA008062S0001503513C glucose labeled Cloned Jurkat cell mid level Noxa cells
SA008063S0001503413C glucose labeled Cloned Jurkat cell mid level Noxa cells
SA008064S0001504513C glucose labeled Cloned Jurkat cell mid level Noxa spent medium
SA008065S0001504613C glucose labeled Cloned Jurkat cell mid level Noxa spent medium
SA008066S0001504713C glucose labeled Cloned Jurkat cell mid level Noxa spent medium
SA008067S0001503813C glucose labeled Noxa overexpressed Jurkat cell cells
SA008068S0001503613C glucose labeled Noxa overexpressed Jurkat cell cells
SA008069S0001503713C glucose labeled Noxa overexpressed Jurkat cell cells
SA008070S0001505013C glucose labeled Noxa overexpressed Jurkat cell spent medium
SA008071S0001504813C glucose labeled Noxa overexpressed Jurkat cell spent medium
SA008072S0001504913C glucose labeled Noxa overexpressed Jurkat cell spent medium
SA008073S0001504113C glutamine labeled Cloned Jurkat cell mid level Noxa cells
SA008074S0001503913C glutamine labeled Cloned Jurkat cell mid level Noxa cells
SA008075S0001504013C glutamine labeled Cloned Jurkat cell mid level Noxa cells
SA008076S0001505113C glutamine labeled Cloned Jurkat cell mid level Noxa spent medium
SA008077S0001505313C glutamine labeled Cloned Jurkat cell mid level Noxa spent medium
SA008078S0001505213C glutamine labeled Cloned Jurkat cell mid level Noxa spent medium
SA008079S0001504413C glutamine labeled Noxa overexpressed Jurkat cell cells
SA008080S0001504313C glutamine labeled Noxa overexpressed Jurkat cell cells
SA008081S0001504213C glutamine labeled Noxa overexpressed Jurkat cell cells
SA008082S0001505613C glutamine labeled Noxa overexpressed Jurkat cell spent medium
SA008083S0001505413C glutamine labeled Noxa overexpressed Jurkat cell spent medium
SA008084S0001505513C glutamine labeled Noxa overexpressed Jurkat cell spent medium
Showing results 1 to 24 of 24

Collection:

Collection ID:CO000152
Collection Summary:-
Sample Type:Culture Media

Treatment:

Treatment ID:TR000171

Sample Preparation:

Sampleprep ID:SP000166
Sampleprep Summary:-
Sampleprep Protocol Filename:Fluxomics_analysis_protocol-2015-03-11.pdf

Combined analysis:

Analysis ID AN000233 AN000234
Analysis type MS MS
Chromatography type HILIC GC
Chromatography system Agilent 1260 Agilent 7890A
Column Phenomenex Luna NH2 (150 x 1mm,3um) Agilent DB5-MS (30m × 0.25mm, 0.25um)
MS Type ESI EI
MS instrument type QTOF Single quadrupole
MS instrument name Agilent 6520 QTOF Agilent 5975C
Ion Mode NEGATIVE POSITIVE
Units Area Area

Chromatography:

Chromatography ID:CH000162
Methods ID:AQM010
Methods Filename:QTOF-002-HILIC-35min-1mm-No_Insert.m.zip
Instrument Name:Agilent 1260
Column Name:Phenomenex Luna NH2 (150 x 1mm,3um)
Chromatography Type:HILIC
  
Chromatography ID:CH001301
Methods ID:AQM011
Methods Filename:ALPHA_KETO_ACIDS-FULL.M.zip
Instrument Name:Agilent 7890A
Column Name:Agilent DB5-MS (30m × 0.25mm, 0.25um)
Chromatography Type:GC

MS:

MS ID:MS000184
Analysis ID:AN000233
Instrument Name:Agilent 6520 QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:NEGATIVE
Acquisition Parameters File:QTOF-002-HILIC-35min-1mm-No_Insert.m.zip
Processing Parameters File:EX00313-MassHunterQuant-LCMS-DataAnalysis-Method.m.zip
  
MS ID:MS000185
Analysis ID:AN000234
Instrument Name:Agilent 5975C
Instrument Type:Single quadrupole
MS Type:EI
Ion Mode:POSITIVE
Acquisition Parameters File:ALPHA_KETO_ACIDS-FULL.M.zip
Processing Parameters File:EX00313-MassHunterQuant-GCMS-DataAnalysis-Method.m.zip
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