Summary of study ST000147

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000129. The data can be accessed directly via it's Project DOI: 10.21228/M85C7W This work is supported by NIH grant, U2C- DK119886.

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Study IDST000147
Study Title13C targeted metabolomics
Study SummaryJurkat parental cells (JP6) or cells stably expressing the pCDNA Noxa vector (N5) cells were cultured to confluence and counted. 20E6 cells in triplicate were resuspended in glucose free or glutamine free medium for 2 hours. Cells were then pelleted and resuspended in 13C-1,2 Glucose (10mM) or 13C-U-glutamine (4mM) for 24 hours. Cells were pelleted and spent medium was collected. Cell pellets were washed 1X with ice cold PBS and cell pellets were resuspended in 400ul -20 methanol. Cells and media were snap frozen in liquid nitrogen and stored at -80.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Email mkachman@umich.edu
Submit Date2015-03-13
Num Groups8
Total Subjects24
Raw Data AvailableYes
Raw Data File Type(s).cd,.cG, .xml, .bin,.stg
Uploaded File Size2.6 G
Analysis Type DetailGC/LC-MS
Release Date2015-03-13
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M85C7W
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000129
Project DOI:doi: 10.21228/M85C7W
Project Title:Metabolic effects of Noxa
Project Summary:labeled glutamine metabolomics on Noxa expressing cells
Institute:University of Minnesota
Department:Masonic Cancer Center
Laboratory:Kelekar Lab
Last Name:Kelekar
First Name:Ameeta
Address:420 Delaware Street SE, Minneapolis, MN 55455
Email:ameeta@umn.edu
Phone:612-626-2358
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