Summary of Study ST000153

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000129. The data can be accessed directly via it's Project DOI: 10.21228/M85C7W This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

Perform statistical analysis  |  Show all samples  |  Show named metabolites  |  Download named metabolite data  
Download mwTab file (text)   |  Download mwTab file(JSON)   |  Download data files (Contains raw data)
Study IDST000153
Study TitlePrimary T Cell Noxa Knockdown (Donor 8)-II
Study SummaryLabeling of cells was carried out in triplicate with each sample containing 35e6. Cells were deprived of glucose or glutamine for 1 h then resuspended in 10mM [13C1,2] D-glucose labeled or 4 mM [13C5]-U-glutamine labeled medium, respectively. At this time cells were also activated with 5 ug/ml anti-CD3 and anti-CD28. Cells were labeled (and activated) for 16 h when samples were spun down, and cells were resuspended in 200 ul ice cold methanol. All samples were immediately stored in -80°C.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Email mkachman@umich.edu
Submit Date2015-02-13
Num Groups3
Total Subjects16
Raw Data AvailableYes
Raw Data File Type(s)d
Uploaded File Size347 M
Analysis Type DetailLC-MS
Release Date2015-04-28
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M85C7W
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Project:

Project ID:PR000129
Project DOI:doi: 10.21228/M85C7W
Project Title:Metabolic effects of Noxa
Project Summary:labeled glutamine metabolomics on Noxa expressing cells
Institute:University of Minnesota
Department:Masonic Cancer Center
Laboratory:Kelekar Lab
Last Name:Kelekar
First Name:Ameeta
Address:420 Delaware Street SE, Minneapolis, MN 55455
Email:ameeta@umn.edu
Phone:612-626-2358

Subject:

Subject ID:SU000172
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Subject Comments:16 subjects
Species Group:Human

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Glucose label Glutamine label
SA008390S00017304no no
SA008391S00017312no no
SA008392S00017313no yes
SA008393S00017298no yes
SA008394S00017303no yes
SA008395S00017305no yes
SA008396S00017302no yes
SA008397S00017300no yes
SA008398S00017299no yes
SA008399S00017301no yes
SA008400S00017311yes no
SA008401S00017310yes no
SA008402S00017308yes no
SA008403S00017306yes no
SA008404S00017307yes no
SA008405S00017309yes no
Showing results 1 to 16 of 16

Collection:

Collection ID:CO000158
Sample Type:Cells

Treatment:

Treatment ID:TR000178

Sample Preparation:

Sampleprep ID:SP000172
Sampleprep Protocol Filename:Fluxomics_analysis_protocol-2015-03-11.docx

Combined analysis:

Analysis ID AN000244
Analysis type MS
Chromatography type HILIC
Chromatography system Agilent 1260
Column Phenomenex Luna NH2 (150 x 1mm, 3um)
MS Type ESI
MS instrument type QTOF
MS instrument name Agilent 6520 QTOF
Ion Mode NEGATIVE
Units Area

Chromatography:

Chromatography ID:CH000169
Methods ID:AQM010
Methods Filename:QTOF-002-HILIC-35min-1mm-No_Insert.m
Instrument Name:Agilent 1260
Column Name:Phenomenex Luna NH2 (150 x 1mm, 3um)
Chromatography Type:HILIC

MS:

MS ID:MS000194
Analysis ID:AN000244
Instrument Name:Agilent 6520 QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:NEGATIVE
Acquisition Parameters File:QTOF-002-HILIC-35min-1mm.m
Processing Parameters File:EX00404-MassHunterQuant-LCMS-Flux-DataAnalysis-Method.m
  logo