Summary of Study ST000155

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000134. The data can be accessed directly via it's Project DOI: 10.21228/M8HP4Z This work is supported by NIH grant, U2C- DK119886.

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Study IDST000155
Study TitleND/HSD sated/fasted fly brains
Study Typediet over time
Study SummaryFlies were fed a normal diet or high-sugar (30% sucrose) diet for 7 days. Brains of flies fasted for 24h or sated were microdissected and immediately frozen in dry ice. The microdissection process from taking the fly out of the vial to freezing the brain took less than a minute. Each brain microdissected piece contains about 50K cells.We dissected 40 brains per condition (4 conditions total) and 4-5 biological replicates
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Email mkachman@umich.edu
Submit Date2015-01-21
Num Groups4
Total Subjects20
Raw Data AvailableYes
Raw Data File Type(s)d
Uploaded File Size895 M
Analysis Type DetailLC-MS
Release Date2015-04-28
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8HP4Z
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000134
Project DOI:doi: 10.21228/M8HP4Z
Project Title:Fly Brain Studies
Project Summary:Measure change in metabolites according to diet and feeding state
Institute:University of Michigan
Department:MCDB LS&A
Laboratory:Dus Lab
Last Name:Dus
First Name:Monica
Address:Ann Arbor, MI
Email:mdus@umich.edu
Phone:347-891-2287

Subject:

Subject ID:SU000174
Subject Type:Insect
Subject Species:Drosophila melanogaster
Taxonomy ID:7227
Species Group:Insect

Factors:

Subject type: Insect; Subject species: Drosophila melanogaster (Factor headings shown in green)

mb_sample_id local_sample_id Condition Diet
SA008433S00017074fasted control
SA008434S00017075fasted control
SA008435S00017076fasted control
SA008436S00017073fasted control
SA008437S00017077fasted control
SA008438S00017087fasted high sugar
SA008439S00017083fasted high sugar
SA008440S00017085fasted high sugar
SA008441S00017084fasted high sugar
SA008442S00017086fasted high sugar
SA008443S00017072sated control
SA008444S00017068sated control
SA008445S00017071sated control
SA008446S00017070sated control
SA008447S00017069sated control
SA008448S00017080sated high sugar
SA008449S00017078sated high sugar
SA008450S00017079sated high sugar
SA008451S00017081sated high sugar
SA008452S00017082sated high sugar
Showing results 1 to 20 of 20

Collection:

Collection ID:CO000160
Collection Summary:-
Sample Type:Tissues

Treatment:

Treatment ID:TR000180

Sample Preparation:

Sampleprep ID:SP000174
Sampleprep Summary:-
Sampleprep Protocol Filename:Gly-TCA-nucleotides_analysis_protocol-2015-03-09.docx

Combined analysis:

Analysis ID AN000247
Analysis type MS
Chromatography type HILIC
Chromatography system Agilent 1260
Column Phenomenex Luna NH2 (150 x 1mm,3um)
MS Type ESI
MS instrument type QTOF
MS instrument name Agilent 6520 QTOF
Ion Mode NEGATIVE
Units pmol/µg protein

Chromatography:

Chromatography ID:CH000172
Methods ID:AQM020
Methods Filename:QTOF-002-HILIC-35min-1mm.m.zip
Instrument Name:Agilent 1260
Column Name:Phenomenex Luna NH2 (150 x 1mm,3um)
Chromatography Type:HILIC

MS:

MS ID:MS000197
Analysis ID:AN000247
Instrument Name:Agilent 6520 QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:NEGATIVE
Acquisition Parameters File:QTOF-002-HILIC-35min-1mm.m.zip
Processing Parameters File:2015210-EX00393-QTOF6520-Dus-GlyTCA-MK-batch.m.zip
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