Summary of Study ST000156

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000135. The data can be accessed directly via it's Project DOI: 10.21228/M8D010 This work is supported by NIH grant, U2C- DK119886.

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Study IDST000156
Study TitleYeast glycolysis in normoxia and hypoxia (150107_pfk2)
Study SummaryThe study is intended to generate the profile of central metabolism, including glycolysis, pentose-phosphate shunt, TCA cycle and nucleotide pools.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Email mkachman@umich.edu
Submit Date2015-01-20
Num Groups15
Total Subjects30
Raw Data AvailableYes
Raw Data File Type(s)d
Uploaded File Size7.1 G
Analysis Type DetailLC-MS
Release Date2015-04-28
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8D010
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000135
Project DOI:doi: 10.21228/M8D010
Project Title:pfk2
Project Summary:Yeast glycolysis in normoxia and hypoxia
Institute:University of Michigan
Department:LSI & Human Genetics
Laboratory:Kim Lab
Last Name:Kim
First Name:John
Address:Ann Arbor, MI
Email:jnkim@umich.edu
Phone:734-615-9915

Subject:

Subject ID:SU000175
Subject Type:Yeast cells
Subject Species:Saccharomyces cerevisiae
Taxonomy ID:4932
Species Group:Microorganism

Factors:

Subject type: Yeast cells; Subject species: Saccharomyces cerevisiae (Factor headings shown in green)

mb_sample_id local_sample_id time strain O2
SA008453S000170390hr 140-165D Plus
SA008454S000170240hr 140-165D Plus
SA008457S000170400hr snf1D Plus
SA008458S000170250hr snf1D Plus
SA008455S000170380hr WT Plus
SA008456S000170230hr WT Plus
SA008459S0001704516hr 140-165D Minus
SA008460S0001703016hr 140-165D Minus
SA008461S0001702716hr 140-165D Plus
SA008462S0001704216hr 140-165D Plus
SA008467S0001703116hr snf1D Minus
SA008468S0001704616hr snf1D Minus
SA008469S0001702816hr snf1D Plus
SA008470S0001704316hr snf1D Plus
SA008463S0001704416hr WT Minus
SA008464S0001702916hr WT Minus
SA008465S0001702616hr WT Plus
SA008466S0001704116hr WT Plus
SA008471S000170518hr 140-165D Minus
SA008472S000170368hr 140-165D Minus
SA008473S000170488hr 140-165D Plus
SA008474S000170338hr 140-165D Plus
SA008479S000170528hr snf1D Minus
SA008480S000170378hr snf1D Minus
SA008481S000170348hr snf1D Plus
SA008482S000170498hr snf1D Plus
SA008475S000170358hr WT Minus
SA008476S000170508hr WT Minus
SA008477S000170328hr WT Plus
SA008478S000170478hr WT Plus
Showing results 1 to 30 of 30

Collection:

Collection ID:CO000161
Collection Summary:-
Sample Type:Saccharomyces cerevisiae

Treatment:

Treatment ID:TR000181

Sample Preparation:

Sampleprep ID:SP000175
Sampleprep Summary:-
Sampleprep Protocol Filename:Gly-TCA-nucleotides_analysis_protocol-2015-03-09.docx

Combined analysis:

Analysis ID AN000248
Analysis type MS
Chromatography type HILIC
Chromatography system Agilent 1260
Column Phenomenex Luna NH2 (150 x 1mm,3um)
MS Type ESI
MS instrument type QTOF
MS instrument name Agilent 6520 QTOF
Ion Mode NEGATIVE
Units

Chromatography:

Chromatography ID:CH000173
Methods ID:AQM020
Methods Filename:QTOF-002-HILIC-35min-1mm.m.zip
Instrument Name:Agilent 1260
Column Name:Phenomenex Luna NH2 (150 x 1mm,3um)
Chromatography Type:HILIC

MS:

MS ID:MS000198
Analysis ID:AN000248
Instrument Name:Agilent 6520 QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:NEGATIVE
Acquisition Parameters File:QTOF-002-HILIC-35min-1mm.m.zip
Processing Parameters File:EX00391-Quant-method.m.zip
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