Summary of Study ST000161
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000140. The data can be accessed directly via it's Project DOI: 10.21228/M8W01B This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000161 |
| Study Title | Plasma Nucleotide/adenosine concentrations (Human AxP Batch 3) |
| Study Type | Exercise and rest |
| Study Summary | This experiment was performed using the following cohorts: 1) healthy controls, 2) patients with scleroderma at low risk for pulmonary hypertension, 3) pateints with scleroderma at high risk for pulmonary hypertension. Whole blood was drawn directly into stop soilution (1:1 ratio) at rest and again at peak exercise for each human subject. |
| Institute | University of Michigan |
| Department | Biomedical Research Core Facilities |
| Laboratory | Metabolomics core |
| Last Name | Kachman |
| First Name | Maureen |
| Address | 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 |
| mkachman@umich.edu | |
| Submit Date | 2014-10-27 |
| Num Groups | 4 |
| Total Subjects | 24 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Uploaded File Size | 225 M |
| Analysis Type Detail | LC-MS |
| Release Date | 2015-04-28 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000140 |
| Project DOI: | doi: 10.21228/M8W01B |
| Project Title: | Human AxP Studies |
| Project Type: | Glycolysis/TCA/Nucleotide analysis (tissue/cells) |
| Project Summary: | Plasma Nucleotide/adenosine concentrations |
| Institute: | University of Michigan |
| Department: | Internal Medicine-Cardiology |
| Laboratory: | Visovatti Lab |
| Last Name: | Visovatti |
| First Name: | Scott |
| Address: | Ann Arbor, MI |
| Email: | shv@med.umich.edu |
| Phone: | 734-657-6917 |
Subject:
| Subject ID: | SU000180 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Human |
Factors:
Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)
| mb_sample_id | local_sample_id | Exercise | Group |
|---|---|---|---|
| SA008587 | S00016694 | no | Healthy |
| SA008588 | S00016708 | no | Healthy |
| SA008589 | S00016692 | no | Healthy |
| SA008590 | S00016696 | no | Healthy |
| SA008591 | S00016688 | no | SSc-high |
| SA008592 | S00016700 | no | SSc-high |
| SA008593 | S00016702 | no | SSc-high |
| SA008594 | S00016704 | no | SSc-high |
| SA008595 | S00016710 | no | SSc-high |
| SA008596 | S00016698 | no | SSc-high |
| SA008597 | S00016690 | no | SSc-high |
| SA008598 | S00016706 | no | SSc-high |
| SA008599 | S00016693 | yes | Healthy |
| SA008600 | S00016709 | yes | Healthy |
| SA008601 | S00016695 | yes | Healthy |
| SA008602 | S00016697 | yes | Healthy |
| SA008603 | S00016707 | yes | SSc-high |
| SA008604 | S00016711 | yes | SSc-high |
| SA008605 | S00016699 | yes | SSc-high |
| SA008606 | S00016689 | yes | SSc-high |
| SA008607 | S00016691 | yes | SSc-high |
| SA008608 | S00016701 | yes | SSc-high |
| SA008609 | S00016703 | yes | SSc-high |
| SA008610 | S00016705 | yes | SSc-high |
| Showing results 1 to 24 of 24 |
Collection:
| Collection ID: | CO000166 |
| Collection Summary: | - |
| Sample Type: | Plasma |
Treatment:
| Treatment ID: | TR000186 |
Sample Preparation:
| Sampleprep ID: | SP000180 |
| Sampleprep Summary: | - |
| Sampleprep Protocol Filename: | Gly-TCA-nucleotides_analysis_protocol-2015-03-09.docx |
Combined analysis:
| Analysis ID | AN000253 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Agilent 1260 |
| Column | Phenomenex Luna NH2 (150 x 1mm,3um) |
| MS Type | ESI |
| MS instrument type | QTOF |
| MS instrument name | Agilent 6520 QTOF |
| Ion Mode | NEGATIVE |
| Units | nM |
Chromatography:
| Chromatography ID: | CH000178 |
| Methods ID: | AQM015 |
| Methods Filename: | QQQ-ATP,ADP,AMP,A-Insert.m.zip |
| Instrument Name: | Agilent 1260 |
| Column Name: | Phenomenex Luna NH2 (150 x 1mm,3um) |
| Chromatography Type: | HILIC |
MS:
| MS ID: | MS000203 |
| Analysis ID: | AN000253 |
| Instrument Name: | Agilent 6520 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
| Acquisition Parameters File: | QQQ-ATP,ADP,AMP,A-Insert.m.zip |
| Processing Parameters File: | EX00370-Nucleotides-Quant.m.zip |
