Summary of Study ST000178
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000153. The data can be accessed directly via it's Project DOI: 10.21228/M8B01C This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000178 |
| Study Title | T cell metabolism during graft-versus-host disease (CAB 307)-PART I |
| Study Type | Glycolysis/TCA/Nucleotide analysis (tissue/cells) |
| Study Summary | T cells were injected into mice to model graft-versus-host disease. On day 7 after bone marrow transplantation (BMT), cells were recovered and CD8 T cells were purified to > 90% purity over magnetic columns. Naïve T cells were used as control. Cells were then plated at 5x10^6 cells/ml onto plates coated with anti-CD3/CD28 antibodies in the presence of 300µM 13C-palmitate conjugated to BSA (in PBS). Cells were incubated at 37oC for 60min, after which time they were removed from the plates, counted, and an equal number (2.5x10^6) were placed into a 1.7 ml micro-centrifuge tubes and spun down. Cells were washed once with ammonium chloride, residual volume was removed by a second brief spin, cell pellets were flash frozen in liquid nitrogen and stored at -80oC until analysis. Several mice lines were used in this study. |
| Institute | University of Michigan |
| Department | Biomedical Research Core Facilities |
| Laboratory | Metabolomics core |
| Last Name | Kachman |
| First Name | Maureen |
| Address | 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 |
| mkachman@umich.edu | |
| Submit Date | 2015-06-06 |
| Num Groups | 2 |
| Total Subjects | 7 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Uploaded File Size | 867 M |
| Analysis Type Detail | GC-MS/LC-MS |
| Release Date | 2016-06-18 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000153 |
| Project DOI: | doi: 10.21228/M8B01C |
| Project Title: | Graft-Versus-Host Disease |
| Project Summary: | T cell metabolism during graft-versus-host disease |
| Institute: | University of Michigan |
| Department: | Pediatrics |
| Laboratory: | Ferrara lab |
| Last Name: | Byersdorfer |
| First Name: | Craig |
| Address: | Ann Arbor, MI |
| Email: | craigbye@med.umich.edu |
| Phone: | 734-000-0000 |
Subject:
| Subject ID: | SU000197 |
| Subject Type: | Animal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Species Group: | Mammal |
Factors:
Subject type: Animal; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Treatment |
|---|---|---|
| SA009490 | S00015099 | Control |
| SA009491 | S00015100 | Control |
| SA009492 | S00015098 | Control |
| SA009493 | S00015097 | Control |
| SA009494 | S00015095 | GVHD |
| SA009495 | S00015096 | GVHD |
| SA009496 | S00015094 | GVHD |
| Showing results 1 to 7 of 7 |
Collection:
| Collection ID: | CO000184 |
| Collection Summary: | - |
| Sample Type: | Cells |
Treatment:
| Treatment ID: | TR000204 |
Sample Preparation:
| Sampleprep ID: | SP000198 |
| Sampleprep Summary: | - |
| Sampleprep Protocol Filename: | Gly-TCA-nucleotides_analysis_protocol-2015-03-09.docx |
Combined analysis:
| Analysis ID | AN000274 | AN000275 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | HILIC | GC |
| Chromatography system | Agilent 1260 | Agilent 7890A |
| Column | Phenomenex Luna NH2 (150 x 1mm,3um) | Agilent DB5-MS (30m × 0.25mm, 0.25um) |
| MS Type | ESI | EI |
| MS instrument type | QTOF | Single quadrupole |
| MS instrument name | Agilent 6530 QTOF | Agilent 5975C |
| Ion Mode | NEGATIVE | POSITIVE |
| Units | pmol/µg protein | pmol/µg protein |
Chromatography:
| Chromatography ID: | CH000196 |
| Methods ID: | AQM020 |
| Methods Filename: | QTOF-002-HILIC-35min-1mm.m.zip |
| Instrument Name: | Agilent 1260 |
| Column Name: | Phenomenex Luna NH2 (150 x 1mm,3um) |
| Chromatography Type: | HILIC |
| Chromatography ID: | CH001304 |
| Methods ID: | AQM011 |
| Methods Filename: | ALPHA_KETO_ACIDS-FULL.M.zip |
| Instrument Name: | Agilent 7890A |
| Column Name: | Agilent DB5-MS (30m × 0.25mm, 0.25um) |
| Chromatography Type: | GC |
MS:
| MS ID: | MS000223 |
| Analysis ID: | AN000274 |
| Instrument Name: | Agilent 6530 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
| Acquisition Parameters File: | Column1_solv1_jetstream+_grad9.m.zip |
| Processing Parameters File: | EX00317-MassHunterQuant-GlyTCA-DataAnalysis-LCMS-Method.m.zip |
| MS ID: | MS000224 |
| Analysis ID: | AN000275 |
| Instrument Name: | Agilent 5975C |
| Instrument Type: | Single quadrupole |
| MS Type: | EI |
| Ion Mode: | POSITIVE |
| Acquisition Parameters File: | ALPHA_KETO_ACIDS-FULL.M.zip |
| Processing Parameters File: | EX00317-MassHunterQuant-GlyTCA-DataAnalysis-GCMS-Method.m.zip |
