Summary of Study ST000185
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000158. The data can be accessed directly via it's Project DOI: 10.21228/M8T01Q This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000185 |
| Study Title | Fetal Lambs vascular graft Normal v Shunt LECs |
| Study Type | Glycolysis/TCA/Nucleotide analysis (tissue/cells) |
| Study Summary | We recently reported superior right ventricle (RV) performance in response to acute afterload challenge in lambs with a model of congenital heart disease with chronic left-to-right cardiac shunts. Compared with control animals, shunt lambs demonstrated increased contractility because of an enhanced Anrep effect (the slow increase in contractility following myocyte stretch). This advantageous physiological response may reflect preservation of a fetal phenotype, since the RV of shunt lambs remains exposed to increased pressure postnatally. Nitric oxide (NO) production by NO synthase (NOS) is activated by myocyte stretch and is a necessary intermediary of the Anrep response. The purpose of this study was to test the hypothesis that NO signaling is increased in the RV of fetal lambs compared with controls and shunt lambs have persistence of this fetal pattern. An 8-mm graft was placed between the pulmonary artery and aorta in fetal lambs (shunt). NOS isoform expression, activity, and association with activating cofactors were determined in fetal tissue obtained during late-gestation and in 4-wk-old juvenile shunt and control lambs. We demonstrated increased RNA and protein expression of NOS isoforms and increased total NOS activity in the RV of both shunt and fetal lambs compared with control. We also found increased NOS activation and association with cofactors in shunt and fetal RV compared with control. These data demonstrate preserved fetal NOS phenotype and NO signaling in shunt RV, which may partially explain the mechanism underlying the adaptive response to increased afterload seen in the RV of shunt lambs. Research is published, core data not used but project description is relevant: http://ajpheart.physiology.org/content/309/1/H157.long |
| Institute | University of Michigan |
| Department | Biomedical Research Core Facilities |
| Laboratory | Metabolomics core |
| Last Name | Kachman |
| First Name | Maureen |
| Address | 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 |
| mkachman@umich.edu | |
| Submit Date | 2015-06-07 |
| Num Groups | 2 |
| Total Subjects | 6 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Uploaded File Size | 512 M |
| Analysis Type Detail | GC-MS/LC-MS |
| Release Date | 2015-12-28 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000158 |
| Project DOI: | doi: 10.21228/M8T01Q |
| Project Title: | LEC metabolomics |
| Project Summary: | Comparison of metabolomic profile in LECs derived from normal and shunt animals |
| Institute: | University of California, San Francisco |
| Department: | Pediatrics |
| Laboratory: | Fineman Lab |
| Last Name: | Fineman |
| First Name: | Jeffrey |
| Address: | San Francisco, CA |
| Email: | jeff.fineman@ucsf.edu |
| Phone: | 415-502-6390 |
Subject:
| Subject ID: | SU000204 |
| Subject Type: | Animal |
| Subject Species: | Ovis aries |
| Taxonomy ID: | 9940 |
| Species Group: | Mammal |
Factors:
Subject type: Animal; Subject species: Ovis aries (Factor headings shown in green)
| mb_sample_id | local_sample_id | Type |
|---|---|---|
| SA009569 | S00017021 | increased flow |
| SA009570 | S00017022 | increased flow |
| SA009571 | S00017020 | increased flow |
| SA009572 | S00017019 | normal control |
| SA009573 | S00017018 | normal control |
| SA009574 | S00017017 | normal control |
| Showing results 1 to 6 of 6 |
Collection:
| Collection ID: | CO000191 |
| Collection Summary: | - |
| Sample Type: | Endothelial Cells |
Treatment:
| Treatment ID: | TR000211 |
Sample Preparation:
| Sampleprep ID: | SP000205 |
| Sampleprep Summary: | - |
| Sampleprep Protocol Filename: | Gly-TCA-nucleotides_analysis_protocol-2015-03-09.docx |
Combined analysis:
| Analysis ID | AN000284 | AN000285 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | HILIC | GC |
| Chromatography system | Agilent 1260 | Agilent 7890A |
| Column | Phenomenex Luna NH2 (150 x 1mm,3um) | Agilent DB5-MS (30m × 0.25mm, 0.25um) |
| MS Type | ESI | EI |
| MS instrument type | QTOF | Single quadrupole |
| MS instrument name | Agilent 6530 QTOF | Agilent 5975C |
| Ion Mode | NEGATIVE | POSITIVE |
| Units | pmol/µg protein | pmol/µg protein |
Chromatography:
| Chromatography ID: | CH000206 |
| Methods ID: | AQM020 |
| Methods Filename: | QTOF-002-HILIC-35min-1mm-No_Insert.m.zip |
| Instrument Name: | Agilent 1260 |
| Column Name: | Phenomenex Luna NH2 (150 x 1mm,3um) |
| Chromatography Type: | HILIC |
| Chromatography ID: | CH001305 |
| Methods ID: | AQM011 |
| Methods Filename: | ALPHA_KETO_ACIDS-FULL.M.zip |
| Instrument Name: | Agilent 7890A |
| Column Name: | Agilent DB5-MS (30m × 0.25mm, 0.25um) |
| Chromatography Type: | GC |
MS:
| MS ID: | MS000233 |
| Analysis ID: | AN000284 |
| Instrument Name: | Agilent 6530 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
| Acquisition Parameters File: | QTOF-002-HILIC-35min-1mm-No_Insert.m.zip |
| MS ID: | MS000234 |
| Analysis ID: | AN000285 |
| Instrument Name: | Agilent 5975C |
| Instrument Type: | Single quadrupole |
| MS Type: | EI |
| Ion Mode: | POSITIVE |
| Acquisition Parameters File: | ALPHA_KETO_ACIDS-FULL.M.zip |
