Summary of Study ST000188
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000159. The data can be accessed directly via it's Project DOI: 10.21228/M8P88W This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000188 |
| Study Title | Murine apyrase |
| Study Summary | This experiment was performed in wild type vs. CD39 knockout (KO) mice that had received infusions of either saline or apyrase over the course of 2 weeks. Whole blood was obtained via intracardiac puncture, and was drawn into a syringe prefilled with an anti-enzyme stop solution. |
| Institute | University of Michigan |
| Department | Biomedical Research Core Facilities |
| Laboratory | Metabolomics core |
| Last Name | Kachman |
| First Name | Maureen |
| Address | 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 |
| mkachman@umich.edu | |
| Submit Date | 2015-06-08 |
| Num Groups | 4 |
| Total Subjects | 15 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Uploaded File Size | 32 M |
| Analysis Type Detail | LC-MS |
| Release Date | 2016-06-18 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000159 |
| Project DOI: | doi: 10.21228/M8P88W |
| Project Title: | Murine AxP |
| Project Summary: | Plasma Nucleotide/adenosine concentrations |
| Institute: | University of Michigan |
| Department: | Internal Medicine-Cardiology |
| Laboratory: | Visovatti Lab |
| Last Name: | Visovatti |
| First Name: | Scott |
| Address: | Ann Arbor, MI |
| Email: | shv@med.umich.edu |
| Phone: | 734-657-6917 |
Subject:
| Subject ID: | SU000207 |
| Subject Type: | Animal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Species Group: | Mammals |
Factors:
Subject type: Animal; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Type | Treatment |
|---|---|---|---|
| SA009614 | S00017521 | KO | apyrase |
| SA009615 | S00017522 | KO | apyrase |
| SA009616 | S00017523 | KO | apyrase |
| SA009617 | S00017525 | KO | apyrase |
| SA009618 | S00017524 | KO | apyrase |
| SA009619 | S00017520 | KO | saline |
| SA009620 | S00017519 | KO | saline |
| SA009621 | S00017518 | KO | saline |
| SA009622 | S00017517 | KO | saline |
| SA009623 | S00017514 | wild type | apyrase |
| SA009624 | S00017515 | wild type | apyrase |
| SA009625 | S00017516 | wild type | apyrase |
| SA009626 | S00017512 | wild type | saline |
| SA009627 | S00017513 | wild type | saline |
| SA009628 | S00017511 | wild type | saline |
| Showing results 1 to 15 of 15 |
Collection:
| Collection ID: | CO000194 |
| Collection Summary: | - |
| Sample Type: | Plasma |
Treatment:
| Treatment ID: | TR000214 |
Sample Preparation:
| Sampleprep ID: | SP000208 |
| Sampleprep Summary: | - |
| Sampleprep Protocol Filename: | Gly-TCA-nucleotides_analysis_protocol-2015-03-09.docx |
Chromatography:
| Chromatography ID: | CH000211 |
| Methods ID: | AQM016 |
| Methods Filename: | QQQ-ATP_ADP_AMP_A-update.m.zip |
| Instrument Name: | Agilent 1260 |
| Column Name: | Phenomenex Luna NH2 (150 x 1mm,3um) |
| Chromatography Type: | HILIC |
Analysis:
| Analysis ID: | AN000289 |
| Laboratory Name: | MRC2 (University of Michigan) |
| Analysis Type: | MS |
| Acquisition ID: | AQM016 |
| Acquisition Parameters File: | QQQ-ATP_ADP_AMP_A-update.m.zip |
| Instrument Name: | Agilent 6530 QTOF |
| Chromatography ID: | CH000211 |
| Num Factors: | 4 |
| Num Metabolites: | 4 |
| Units: | nM |
