Summary of Study ST000189
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000160. The data can be accessed directly via it's Project DOI: 10.21228/M8JG6Z This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000189 |
Study Title | Hirschprung Enterocolitis SCFA |
Study Type | Short-chain fatty acid analysis |
Study Summary | Children with Hirschsprung disease (HD) who have a history of enterocolitis (HAEC) have a shift in colonic microbiota, many of which are necessary for short chain fatty acid (SCFA) production. As SCFAs play a critical role in colonic mucosal preservation, we hypothesized that fecal SCFA composition is altered in children with HAEC. Patients between age of 6 weeks and 16 years of age who were undergoing small bowel resection were selected, including those who were either receiving enteral nutrition preoperatively (control group), or those who were without enteral nutrition and receiving total parenteral nutrition (TPN group). At the time of operation, 100-500µl of effluent from the small bowel lumen was aspirated during the small bowel resection. The samples were immediately frozen in liquid nitrogen and stored at -80oC. Research is published: http://www.sciencedirect.com/science/article/pii/S0022346815006211 |
Institute | University of Michigan |
Department | Biomedical Research Core Facilities |
Laboratory | Metabolomics core |
Last Name | Kachman |
First Name | Maureen |
Address | 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 |
mkachman@umich.edu | |
Submit Date | 2015-06-08 |
Num Groups | 1 |
Total Subjects | 20 |
Raw Data Available | Yes |
Raw Data File Type(s) | d |
Uploaded File Size | 32 M |
Analysis Type Detail | GC-MS |
Release Date | 2016-06-18 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
Project ID: | PR000160 |
Project DOI: | doi: 10.21228/M8JG6Z |
Project Title: | Hirschprung Associated Enterocolitis Studies |
Project Summary: | Assess for correlations between SCFA content and history of HAEC |
Institute: | University of Michigan |
Department: | Surgery |
Laboratory: | Teitelbaum Lab |
Last Name: | Teitelbaum |
First Name: | Daniel |
Address: | Ann Arbor, MI |
Email: | dttbm@umich.edu |
Phone: | 734-936-8464 |
Subject:
Subject ID: | SU000208 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Species Group: | Mammals |
Factors:
Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)
mb_sample_id | local_sample_id | Human Stool |
---|---|---|
SA009629 | S00015499 | Human Stool |
SA009630 | S00015498 | Human Stool |
SA009631 | S00015497 | Human Stool |
SA009632 | S00015496 | Human Stool |
SA009633 | S00015500 | Human Stool |
SA009634 | S00015501 | Human Stool |
SA009635 | S00015504 | Human Stool |
SA009636 | S00015503 | Human Stool |
SA009637 | S00015502 | Human Stool |
SA009638 | S00015495 | Human Stool |
SA009639 | S00015494 | Human Stool |
SA009640 | S00015488 | Human Stool |
SA009641 | S00015487 | Human Stool |
SA009642 | S00015486 | Human Stool |
SA009643 | S00015489 | Human Stool |
SA009644 | S00015490 | Human Stool |
SA009645 | S00015493 | Human Stool |
SA009646 | S00015492 | Human Stool |
SA009647 | S00015491 | Human Stool |
SA009648 | S00015485 | Human Stool |
Showing results 1 to 20 of 20 |
Collection:
Collection ID: | CO000196 |
Collection Summary: | - |
Sample Type: | Feces |
Treatment:
Treatment ID: | TR000216 |
Sample Preparation:
Sampleprep ID: | SP000210 |
Sampleprep Summary: | - |
Sampleprep Protocol Filename: | SCFA_analysis_protocol-2015-03-10.docx |
Combined analysis:
Analysis ID | AN000290 |
---|---|
Analysis type | MS |
Chromatography type | GC |
Chromatography system | |
Column | Phenomenex Zebron ZB-WAXplus (30m x 0.25mm,0.25um) |
MS Type | EI |
MS instrument type | Single quadrupole |
MS instrument name | Agilent 5975C |
Ion Mode | POSITIVE |
Units | pmol/mg |
Chromatography:
Chromatography ID: | CH000212 |
Methods ID: | AQM030 |
Methods Filename: | WAXPLUS_100-200C_2_SIM.txt |
Column Name: | Phenomenex Zebron ZB-WAXplus (30m x 0.25mm,0.25um) |
Chromatography Type: | GC |
MS:
MS ID: | MS000239 |
Analysis ID: | AN000290 |
Instrument Name: | Agilent 5975C |
Instrument Type: | Single quadrupole |
MS Type: | EI |
Ion Mode: | POSITIVE |
Acquisition Parameters File: | WAXPLUS_100-200C_2_SIM.txt |
Processing Parameters File: | EX00332-SCFA-Quant-methods.m.zip |