Summary of Study ST000193
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000164. The data can be accessed directly via it's Project DOI: 10.21228/M81G69 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000193 |
| Study Title | BAF60a LKO Liver specific knockout Bile acid |
| Study Summary | Twelve weeks old WT and BAF60a Liver specfific knockout mice were fed with high cholesterol diet for 8 weeks. Mice were sacrified after 4 h of starvation. Blood samples were harvested by heart puncture for plasma. Liver were dissected and immediately frozen in liquid nitrogen. All the samples were kept in -80oC freezer for long term storage |
| Institute | University of Michigan |
| Department | Biomedical Research Core Facilities |
| Laboratory | Metabolomics core |
| Last Name | Kachman |
| First Name | Maureen |
| Address | 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 |
| mkachman@umich.edu | |
| Submit Date | 2015-06-09 |
| Num Groups | 2 |
| Total Subjects | 18 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Uploaded File Size | 64 M |
| Analysis Type Detail | LC-MS |
| Release Date | 2015-12-28 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000164 |
| Project DOI: | doi: 10.21228/M81G69 |
| Project Title: | BAF60a LKO Liver specific knockout Bile acid |
| Project Summary: | Regulation of Bile acid metabolism by hepatic BAF60a |
| Institute: | University of Michigan |
| Department: | Life Sciences Institute |
| Laboratory: | Lin Lab |
| Last Name: | Lin |
| First Name: | Jiandie |
| Address: | Ann Arbor, MI |
| Email: | jdlin@umich.edu |
| Phone: | 734-615-3512 |
Subject:
| Subject ID: | SU000212 |
| Subject Type: | Animal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Species Group: | Mammal |
Factors:
Subject type: Animal; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Genotype |
|---|---|---|
| SA009748 | S00014262 | Knockout |
| SA009749 | S00014253 | Knockout |
| SA009750 | S00014267 | Knockout |
| SA009751 | S00014268 | Knockout |
| SA009752 | S00014270 | Knockout |
| SA009753 | S00014259 | Knockout |
| SA009754 | S00014261 | Knockout |
| SA009755 | S00014258 | Knockout |
| SA009756 | S00014256 | Wild type |
| SA009757 | S00014255 | Wild type |
| SA009758 | S00014254 | Wild type |
| SA009759 | S00014266 | Wild type |
| SA009760 | S00014269 | Wild type |
| SA009761 | S00014265 | Wild type |
| SA009762 | S00014257 | Wild type |
| SA009763 | S00014263 | Wild type |
| SA009764 | S00014264 | Wild type |
| SA009765 | S00014260 | Wild type |
| Showing results 1 to 18 of 18 |
Collection:
| Collection ID: | CO000200 |
| Collection Summary: | - |
| Sample Type: | Liver |
Treatment:
| Treatment ID: | TR000220 |
Sample Preparation:
| Sampleprep ID: | SP000214 |
| Sampleprep Summary: | - |
| Sampleprep Protocol Filename: | Bile-acids-2015-03-11.docx |
Combined analysis:
| Analysis ID | AN000295 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | |
| Column | Waters Acquity BEH C18 (50 x 2.1mm,1.7um) |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | Agilent 6490 QQQ |
| Ion Mode | NEGATIVE |
| Units | nmol |
Chromatography:
| Chromatography ID: | CH000216 |
| Methods ID: | AQM040 |
| Methods Filename: | 2014-03-27-Bile_acid-full-ESN-insert.m.zip |
| Column Name: | Waters Acquity BEH C18 (50 x 2.1mm,1.7um) |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS000244 |
| Analysis ID: | AN000295 |
| Instrument Name: | Agilent 6490 QQQ |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
| Acquisition Parameters File: | 2014-03-27-Bile_acid-full-ESN-insert.m.zip |
| Processing Parameters File: | EX00282-Bile-Acids-Quant-method.m.zip |
