Summary of Study ST000193
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000164. The data can be accessed directly via it's Project DOI: 10.21228/M81G69 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000193 |
Study Title | BAF60a LKO Liver specific knockout Bile acid |
Study Summary | Twelve weeks old WT and BAF60a Liver specfific knockout mice were fed with high cholesterol diet for 8 weeks. Mice were sacrified after 4 h of starvation. Blood samples were harvested by heart puncture for plasma. Liver were dissected and immediately frozen in liquid nitrogen. All the samples were kept in -80oC freezer for long term storage |
Institute | University of Michigan |
Department | Biomedical Research Core Facilities |
Laboratory | Metabolomics core |
Last Name | Kachman |
First Name | Maureen |
Address | 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 |
mkachman@umich.edu | |
Submit Date | 2015-06-09 |
Num Groups | 2 |
Total Subjects | 18 |
Raw Data Available | Yes |
Raw Data File Type(s) | d |
Uploaded File Size | 64 M |
Analysis Type Detail | LC-MS |
Release Date | 2015-12-28 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
Project ID: | PR000164 |
Project DOI: | doi: 10.21228/M81G69 |
Project Title: | BAF60a LKO Liver specific knockout Bile acid |
Project Summary: | Regulation of Bile acid metabolism by hepatic BAF60a |
Institute: | University of Michigan |
Department: | Life Sciences Institute |
Laboratory: | Lin Lab |
Last Name: | Lin |
First Name: | Jiandie |
Address: | Ann Arbor, MI |
Email: | jdlin@umich.edu |
Phone: | 734-615-3512 |
Subject:
Subject ID: | SU000212 |
Subject Type: | Animal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Species Group: | Mammals |
Factors:
Subject type: Animal; Subject species: Mus musculus (Factor headings shown in green)
mb_sample_id | local_sample_id | Genotype |
---|---|---|
SA009748 | S00014262 | Knockout |
SA009749 | S00014253 | Knockout |
SA009750 | S00014267 | Knockout |
SA009751 | S00014268 | Knockout |
SA009752 | S00014270 | Knockout |
SA009753 | S00014259 | Knockout |
SA009754 | S00014261 | Knockout |
SA009755 | S00014258 | Knockout |
SA009756 | S00014256 | Wild type |
SA009757 | S00014255 | Wild type |
SA009758 | S00014254 | Wild type |
SA009759 | S00014266 | Wild type |
SA009760 | S00014269 | Wild type |
SA009761 | S00014265 | Wild type |
SA009762 | S00014257 | Wild type |
SA009763 | S00014263 | Wild type |
SA009764 | S00014264 | Wild type |
SA009765 | S00014260 | Wild type |
Showing results 1 to 18 of 18 |
Collection:
Collection ID: | CO000200 |
Collection Summary: | - |
Sample Type: | Liver |
Treatment:
Treatment ID: | TR000220 |
Sample Preparation:
Sampleprep ID: | SP000214 |
Sampleprep Summary: | - |
Sampleprep Protocol Filename: | Bile-acids-2015-03-11.docx |
Combined analysis:
Analysis ID | AN000295 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | |
Column | Waters Acquity BEH C18 (50 x 2.1mm,1.7um) |
MS Type | ESI |
MS instrument type | Triple quadrupole |
MS instrument name | Agilent 6490 QQQ |
Ion Mode | NEGATIVE |
Units | nmol |
Chromatography:
Chromatography ID: | CH000216 |
Methods ID: | AQM040 |
Methods Filename: | 2014-03-27-Bile_acid-full-ESN-insert.m.zip |
Column Name: | Waters Acquity BEH C18 (50 x 2.1mm,1.7um) |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS000244 |
Analysis ID: | AN000295 |
Instrument Name: | Agilent 6490 QQQ |
Instrument Type: | Triple quadrupole |
MS Type: | ESI |
Ion Mode: | NEGATIVE |
Acquisition Parameters File: | 2014-03-27-Bile_acid-full-ESN-insert.m.zip |
Processing Parameters File: | EX00282-Bile-Acids-Quant-method.m.zip |