Summary of Study ST000194
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000165. The data can be accessed directly via it's Project DOI: 10.21228/M8WS31 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000194 |
| Study Title | Lanthanide-mineral induced alteration of bile acid metabolism in a murine model of steatohepatitis |
| Study Summary | 120 mice (equal number of males and females) were randomized into 3 equal groups. One group is on a HFWD alone, a second group is on HFWD supplemented with lanthanides and calcium, and a third control group is supplemented with calcium alone. At study termination (18 months) we will harvest hepatocytes and colonic enterocytes for evaluation of epithelial gene expression patterns. We will also harvest cecal contents and feces for microbial profiling by 16S rRNA pyrosequencing. For this small pilot proposal, we wish to add an untargeted metabolomic analysis component. We will harvest liver (right medial lobe with gall bladder), serum, and feces. We will assay representative liver/gall bladder samples (8 from the HFWD group and 8 from the lanthanide/calcium supplemented group). Remaining liver samples and the serum and feces will be archived for future investigation. Liver is being targeted first since both steatohepatitis and hepatocellular carcinoma were seen in our previous study in mice on HFWD [2]. Additionally, alterations of bile acid profiles and bile acid metabolism have been associated with both steatohepatitis and hepatic cancers. |
| Institute | University of Michigan |
| Department | Biomedical Research Core Facilities |
| Laboratory | Metabolomics core |
| Last Name | Kachman |
| First Name | Maureen |
| Address | 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 |
| mkachman@umich.edu | |
| Submit Date | 2015-06-09 |
| Num Groups | 20 |
| Total Subjects | 20 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Uploaded File Size | 64 M |
| Analysis Type Detail | LC-MS |
| Release Date | 2016-06-18 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000165 |
| Project DOI: | doi: 10.21228/M8WS31 |
| Project Title: | Tumor Suppression |
| Project Summary: | Tumor suppression by diet in mice fed HFWD |
| Institute: | University of Michigan |
| Department: | Animal Medicine |
| Laboratory: | Bergin Lab |
| Last Name: | Bergin |
| First Name: | Ingrid |
| Address: | Ann Arbor, MI |
| Email: | ingridbe@med.umich.edu |
| Phone: | 734-936-1701 |
Subject:
| Subject ID: | SU000213 |
| Subject Type: | Animal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Species Group: | Mammal |
Factors:
Subject type: Animal; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Diet |
|---|---|---|
| SA009775 | S00015181 | HFWD |
| SA009776 | S00015182 | HFWD |
| SA009777 | S00015183 | HFWD |
| SA009778 | S00015184 | HFWD |
| SA009779 | S00015175 | HFWD |
| SA009780 | S00015176 | HFWD |
| SA009781 | S00015177 | HFWD |
| SA009782 | S00015178 | HFWD |
| SA009783 | S00015179 | HFWD |
| SA009784 | S00015180 | HFWD |
| SA009766 | S00015185 | HFWD + Calcium |
| SA009767 | S00015186 | HFWD + Calcium |
| SA009768 | S00015191 | HFWD + Calcium |
| SA009769 | S00015187 | HFWD + Calcium |
| SA009770 | S00015193 | HFWD + Calcium |
| SA009771 | S00015192 | HFWD + Calcium |
| SA009772 | S00015188 | HFWD + Calcium |
| SA009773 | S00015189 | HFWD + Calcium |
| SA009774 | S00015190 | HFWD + Calcium |
| Showing results 1 to 19 of 19 |
Collection:
| Collection ID: | CO000201 |
| Collection Summary: | - |
| Sample Type: | Liver |
Treatment:
| Treatment ID: | TR000221 |
Sample Preparation:
| Sampleprep ID: | SP000215 |
| Sampleprep Summary: | - |
| Sampleprep Protocol Filename: | Bile-acids-2015-03-11.docx |
Combined analysis:
| Analysis ID | AN000296 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | |
| Column | Waters Acquity BEH C18 (50 x 2.1mm,1.7um) |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | Agilent 6490 QQQ |
| Ion Mode | NEGATIVE |
| Units | pmol/mg sample |
Chromatography:
| Chromatography ID: | CH000217 |
| Methods ID: | AQM041 |
| Methods Filename: | 2014-07-30-Bile_acid-full-BEH-C18-h.m.zip |
| Column Name: | Waters Acquity BEH C18 (50 x 2.1mm,1.7um) |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS000245 |
| Analysis ID: | AN000296 |
| Instrument Name: | Agilent 6490 QQQ |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
| Acquisition Parameters File: | 2014-07-30-Bile_acid-full-BEH-C18-h.m.zip |
